Three Glycosylated Polypeptides Secreted by Several Embryogenic Cell Cultures of Pine Show Highly Specific Serological Affinity to Antibodies Directed against the Wheat Germin Apoprotein Monomer

Domon, Jean-Marc; Dumas, Bernard; Lainé, Éric; Meyer, Yves; David, Alain; David, Hélène N.

doi:10.1104/pp.108.1.141

Cited by 82 publications

(41 citation statements)

References 21 publications

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“…Domon et al [57] compared profiles of secreted proteins, which were detected on the surface of preglobulary somatic embryos of four embryogenic cell lines of Pinus caribaea and those, on the contrary to our results, were very similar. This study was based on literature presenting the hypothesis that chitinases are potential markers for embryogenesis, confirmed by differences in extracellular protein profiles among embryogenic cell lines with different embryogenic capacity [17,58] and also between embryogenic and nonembryogenic cell lines [18,[23][24][25]57,[59][60][61]. However, our results showed that in the 18 embryogenic cell lines of P. nigra and the seven cell lines of hybrid firs tested, the presence or absence of any one detected isoform cannot be distinctly associated with the high or low embryogenic potential of the tested cell lines.…”

Section: Discussioncontrasting

confidence: 97%

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

et al. 2013

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Glucanase and chitinase enzymes play an important role in different plant processes including defense against pathogens and morphogenesis. Moreover, their role in the processes of somatic embryogenesis has been demonstrated. It has been suggested, that the presence of this type of proteins might be a marker for embryogenic potential of callus cultures. In this work we screened for the presence of glucanases and chitinases in liquid growth media of a set of conifer embryogenic cell lines in order to find correlation with their embryogenic potential. We have found that none of the 12 chitinase isoforms detected in culture media of Pinus nigra Arn. or the nine chitinases detected in media with Abies alba × A. cephalonica and Abies alba × A. numidica embryogenic tissues could be linked to their embryogenic capacity. Similarly, none of the six glucanase isoforms detected in the extracellular fluid of Pinus nigra Arn. cultures can be assigned as a marker of embryogenic potential. Thus, our data indicate the large variability and doubtless importance of glucanases and chitinases for cell growth and development of somatic embryos, however, do not support the premise that they are markers of embryogenesis.

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Section: Discussioncontrasting

confidence: 97%

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

et al. 2013

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“…However, it is known that GLPs are glycosylated (33). All GLPs, including nectarin I, contain a conserved site of Nglycosylation (2,10,11,16,17,19,33). PAS staining (28) demonstrated the presence of carbohydrate on the purified nectarin I protein (Fig.…”

Section: Resultsmentioning

confidence: 99%

Tobacco Nectarin I

Carter

Thornburg

2000

Journal of Biological Chemistry

193

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Nectarin I, a protein that accumulates in the nectar of Nicotiana sp., was determined to contain superoxide dismutase activity by colorimetric and in-gel assays. This activity was found to be remarkably thermostable. did not restore superoxide dismutase activity. The quaternary structure of the reconstituted enzyme was examined, and only tetrameric and pentameric aggregates were enzymatically active. The reconstituted enzyme was also shown to generate H 2 O 2 . Putative nectarin I homologues were found in the nectars of several other plant species.

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“…Germin was first detected in germinating cereals, but subsequently, germin-like proteins were also identified in a protist (Lane, 1991), dicotyledonous angiosperms (Delseny et al, 1994;Heintzen et al, 1994), and gymnosperms (Domon et al, 1995). Germins and germin-like proteins constitute a large family of proteins ubiquitously distributed in the plant kingdom.…”

Section: Introductionmentioning

confidence: 99%

Localization of Germin Genes and Their Products in Developing Wheat Coleoptiles

Çalışkan¹,

Özcan²,

Turan³

et al. 2004

BMB Reports

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Germination is a process which characterized with nescient synthesis of genes. Among the genes synthesized during the germination of wheat embryos, germin genes, proteins and their enzymatic activity were defined. Germin is a water soluble homopentameric glycoprotein which is remarkable resistant to degradation by a broad range of proteases including pepsin. Germin proteins found to have strong oxalate oxidase activity which produces hydrogen peroxide by degrading oxalic acid. The current study, aimed to localize the germin genes, proteins and enzymatic activities in developing coleoptiles which is a rapidly growing protective tissue of leaf primordium and shoot apex. Non-radioactively labeled germin riboprobes were employed to localize germin mRNAs in situ. FITC (Fluorescein isothiocyanate) and alkaline phosphatase linked anti-germin antibodies were used to localize germin proteins under the fluorescence and light microscopy and finally germin enzymatic activity was localized by using appropriate enzyme assay. The results revealed that in coleoptiles germin genes, proteins and their enzymatic activity were predominantly associated with the cells of epidermis and vascular bundle sheath cells.

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Three Glycosylated Polypeptides Secreted by Several Embryogenic Cell Cultures of Pine Show Highly Specific Serological Affinity to Antibodies Directed against the Wheat Germin Apoprotein Monomer

Cited by 82 publications

References 21 publications

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

Tobacco Nectarin I

Localization of Germin Genes and Their Products in Developing Wheat Coleoptiles

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