Three-fluorescence sensor for minute-time scale low-cost analysis of urinary oxalate in urolithiasis metabolic assessment

Xin, Jiang; Bai, Yunjin; Liu, Qilin; Yan, Li; Long, Tingting; Li, Mei; Huang, Jin; Ying, Binwu; Chen, Piaopiao

doi:10.1016/j.aca.2022.340586

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Therefore, the detection of oxalate ions in blood and urine is of great significance for monitoring renal function, assessing the risk of cardiovascular diseases, diagnosing gout, and detecting urinary stone formation. Currently available methods for oxalate ion detection include enzymatic methods [ 12 ], high-performance liquid chromatography (HPLC) [ 13 ], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [ 14 , 15 ], electrochemical methods [ 16 ], fluorescence spectroscopy [ 17 ], ultraviolet-visible spectroscopy (UV-Vis) [ 18 ], and capillary electrophoresis (CE) [ 19 ]. Enzymatic analysis exhibits high selectivity but is limited by the susceptibility of enzyme activity to environmental factors.…”

Section: Introductionmentioning

confidence: 99%

Solid Electrochemiluminescence Sensor by Immobilization of Emitter Ruthenium(II)tris(bipyridine) in Bipolar Silica Nanochannel Film for Sensitive Detection of Oxalate in Serum and Urine

Yu,

Zhao,

Liu

2024

Nanomaterials

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Convenient and highly sensitive detection of oxalate ions in body fluids is of crucial significance for disease prevention, diagnosis, and monitoring of treatment effectiveness. Establishing a simple solid-state electrochemiluminescence (ECL) sensing system for highly sensitive detection of oxalate ions is highly desirable. In this work, a solid ECL sensor was fabricated by immobilizing the commonly used emitter ruthenium(II)tris(bipyridine) (Ru(bpy)32+) on a double-layered bipolar silica nanochannel array film (bp-SNA)-modified electrode, enabling sensitive detection of oxalate ions in serum or urine samples. Cost-effective and readily available indium tin oxide (ITO) was used as the supporting electrode. Convenient fabrication of multiple negatively charged SNA (n-SNA)-modified ITO electrodes was achieved through the one-step Stöber solution growth method. Subsequently, a positive outer layer film (p-SNA) was rapidly prepared using an electrochemical-assisted self-assembly method. The double-layered bipolar silica nanochannel array film achieved stable immobilization of Ru(bpy)32+ on the electrode surface, facilitated by the electrostatic adsorption of Ru(bpy)32+ by n-SNA and the electrostatic repulsion by p-SNA. Utilizing oxalate ions as a co-reactant for Ru(bpy)32+, combined with the electrostatic enrichment of oxalate ions by p-SNA, the constructed sensor enabled highly sensitive detection of oxalate ions ranging from 1 nM to 25 μM and from 25 μM to 1 mM, with a detection limit (LOD) of 0.8 nM. The fabricated ECL sensor exhibited high selectivity and good stability, making it suitable for ECL detection of oxalate ions in serum and urine samples.

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