Three evolutionary lineages of tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici, based on sequences of IGS, MAT1, and pg1, are each composed of isolates of a single mating type and a single or closely related vegetative compatibility group

Variation and Phylogeny of Fusarium oxysporum Isolates Based on Nucleotide Sequences of Polygalacturonase Genes

“…In all trees, isolates of each race of F. oxysporum f. sp. lycopersici formed a cluster, which was consistent with previous reports (12,13).…”

“…lilii, tulipae, cucumerinum, lagenariae, and niveum had monophyletic origin. However, most of the other formae speciales, such as, cubense, melonis, asparagi, dianthi, gladioli, lini, and lycopersici, have been revealed to have multiple evolutionary origins; in other words, they are polyphyletic (4,10,13,22). No stable correlation between phylogeny and pathogenic-group has been reported for F. oxysporum (5,11,13,16,30).…”

“…However, phylogenetic analyses using these molecular markers have shown that each forma specialis often has multiple phylogenetic origins, and race and phylogeny do not always correlate with each other (1,8,17,20,31). Kawabe et al (13) determined that there were three evolutionary lineages of F. oxysporum f. sp. lycopersici (FOL) among present isolates collected worldwide, based on the nucleotide sequences of rDNA IGS and MAT1, as well as pg1, and confirmed that FOL has multiple phylogenetic origins.…”

GMC oxidoreductase, a highly expressed protein in a potent biocontrol agent Fusarium oxysporum Cong:1-2, is dispensable for biocontrol activity

“…Purifi ed proteins (50 μg) were applied on a strip (Imobiline DryStrip pH3-10 NL, GE Healthcare) and were separated by electrophoresis on an IPGphor apparatus (GE Healthcare) for a total of 35,999 kV hours. (Kawabe et al, 2005). Plasmid DNA was purifi ed from Escherichia coli using QIAGEN Plasmid Midi (QIAGEN, Germantown, MD, USA) or Plasmid Miniprep (Bio-Rad, Hercules, CA, USA).…”

Tomato as a model plant for plant-pathogen interactions

“…Phylogenetic study showed that molecular diversity among the isolates was highest in Manatee County, FL, suggesting that Manatee County is the center of origin of race 3. Kawabe et al (2005) performed a phylogenetic analysis based on sequences of the ribosomal DNA intergenic spacer (IGS) region using a worldwide collection of F. oxysporum isolates, and found three evolutionary lineages (A1, A2, and A3) of FOL among the isolates. They found that each lineage consisted of isolates mainly belonging to a single or closely related vegetative compatibility group (VCG) and having a single mating type (MAT).…”