Three distinct begomoviruses associated with soybean in central Brazil

Fernandes, Fernanda Rausch; Cruz, A. R. R.; Faria, J. C.; Zerbini, F. Murilo; Aragão, F. J. L.

doi:10.1007/s00705-009-0463-0

Cited by 37 publications

(22 citation statements)

References 15 publications

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“…It is believed that begomoviruses infecting wild hosts in Brazil have been horizontally transferred to crop plants, and that in the new host they can adapt by mutation, recombination or pseudorecombination, eventually emerging as novel species (Castillo‐Urquiza et al. , 2008; Fernandes et al. , 2009).…”

Section: Introductionmentioning

confidence: 99%

Species diversity, phylogeny and genetic variability of begomovirus populations infecting leguminous weeds in northeastern Brazil

et al. 2011

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A survey of begomoviruses infecting leguminous weeds (family Fabaceae) was carried out in four states of northeastern Brazil. A total of 26 full-length begomovirus components (19 DNA-A and seven DNA-B, with three pairs of cognate A and B components) were amplified using rolling-circle amplification, then cloned and sequenced. Sequence analysis indicated the presence of six species, four of them novel. In phylogenetic analysis five of the viruses clustered with other Brazilian begomoviruses, but one of them (Euphorbia yellow mosaic virus, EuYMV) clustered with viruses from other countries in Central and South America. Evidence of recombination was found among isolates of Macroptilium yellow spot virus (MaYSV). The MaYSV population had a high degree of genetic variability. Macroptilium lathyroides was revealed as a common host for several of these viruses, and could act as a mixing vessel from which recombinant viruses could emerge. The results indicate that leguminous weeds are reservoirs of several begomoviruses in Brazil, and could play a significant role in begomovirus epidemics, both as inoculum sources and as sources of emerging novel viruses.

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Section: Introductionmentioning

confidence: 99%

Species diversity, phylogeny and genetic variability of begomovirus populations infecting leguminous weeds in northeastern Brazil

et al. 2011

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“…Isolates were selected based on crop, collection location and sequence of the 1.5 kb DNA‐A PCR product for full‐length sequencing. Based on the alignment of the 1.5 kb PCR products and nucleotide sequences obtained from GenBank (Table S1; Alabi et al , ; Barnabas et al , ; Blair et al , ; Durham et al , , Fernandes et al , ; Gharsallah Chouchane et al , ; Gilbertson et al , ; Hameed & Robinson, ; Morinaga et al , ; Pant et al , ; Rouhibakhsh et al , ; Singh et al , ; Usharani et al , ; Yadav et al , ; Zaim et al , ), abutting primer pairs were designed to amplify the full‐length DNA‐As and DNA‐Bs of MYMIV and MYMV isolates from Indonesia and Vietnam, respectively (Table S2). Full‐length viral DNA‐A and DNA‐B were amplified from each selected sample by PCR as previously described (Tsai et al , 2011 b ).…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity of legume yellow mosaic begomoviruses in Indonesia and Vietnam

Tsai

Shih

Rauf

et al. 2013

Annals of Applied Biology

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High incidences of yellow mosaic symptoms were observed in soybean and yard-long bean crops in Indonesia in 2009 and in mungbean crops in Vietnam in 2011. All five soybean and 20 yard-long bean samples from Java, Indonesia, and 15 mungbean samples from Vietnam with symptoms tested positive for begomovirus infection by polymerase chain reaction (PCR) with primer pair PAL1v1978B/PAR1c715H. On the basis of collection location and the nucleotide sequence comparisons of the 1.5 kb begomoviral DNA-A components amplified, a subset of samples comprising two soybean and six yard-long bean isolates from Indonesia and five mungbean isolates from Vietnam were taken forward for more detailed examination. Sequence comparison and phylogenetic analysis of the full-length sequences of all Indonesian and Vietnam isolates alongside other legume-infecting begomoviruses revealed that all the isolates from Indonesia were Mungbean yellow mosaic India virus (MYMIV) strain-A, and all from Vietnam were Mungbean yellow mosaic virus (MYMV) strain-B. To the best of our knowledge, this is the first identification of MYMIV and MYMV associated with yellow mosaic of legumes in Indonesia and Vietnam, respectively. The epidemiological implications and potential consequences of the emergence of legume-infecting begomoviruses on legume production in these areas of Southeast Asia are discussed.

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“…BGMV occurred at the highest incidence followed by SbBMV and ToYSV. Three distinct begomoviruses, i.e., BGMV, Sida micrantha mosaic virus, and Okra mottle virus, have been reported to naturally infect soybean sporadically in the Central Brazil based on phylogenetic analysis of DNA-A sequences (Fernandes, Cruz, Faria, Zerbini, & Aragao, 2009). Furthermore, the virus that induced chlorotic spots on soybean leaves in Brazil was identified as a novel begomovirus for which SoCSV name was proposed (Coco et al, 2013).…”

Section: Brazilian Bud Blightmentioning

confidence: 98%