Three-dimensional visualization of in vitro cultivated chondrocytes inside porous gelatine scaffolds: A tomographic approach

Zehbe, Rolf; Goebbels, Jürgen; Ibold, Yvonne; Groß, Ulrich; Schubert, H.

doi:10.1016/j.actbio.2009.11.020

Cited by 26 publications

(29 citation statements)

References 38 publications

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“…Cells seeded on porous scaffolds can also been identified following osmium tetroxide staining and drying allowing study of cell location and distribution [181]. Synchrotron-based μCT has been implemented to visualize individual cartilage cells on porous scaffolds by staining with Au/Ag [182]. μCT has also been employed to monitor a tissue engineering scaffold implanted in the abdomen of rats for six months [183].…”

Section: X-ray Imagingmentioning

confidence: 99%

Imaging challenges in biomaterials and tissue engineering

et al. 2013

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Biomaterials are employed in the fields of tissue engineering and regenerative medicine (TERM) in order to enhance the regeneration or replacement of tissue function and/or structure. The unique environments resulting from the presence of biomaterials, cells, and tissues result in distinct challenges in regards to monitoring and assessing the results of these interventions. Imaging technologies for three-dimensional (3D) analysis have been identified as a strategic priority in TERM research. Traditionally, histological and immunohistochemical techniques have been used to evaluate engineered tissues. However, these methods do not allow for an accurate volume assessment, are invasive, and do not provide information on functional status. Imaging techniques are needed that enable non-destructive, longitudinal, quantitative, and three-dimensional analysis of TERM strategies. This review focuses on evaluating the application of available imaging modalities for assessment of biomaterials and tissue in TERM applications. Included is a discussion of limitations of these techniques and identification of areas for further development.

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Section: X-ray Imagingmentioning

confidence: 99%

Imaging challenges in biomaterials and tissue engineering

et al. 2013

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“…7. 18 However, an accumulation of cells at the superficial zone of the scaffold became obvious (Fig. 6d).…”

Section: Resultsmentioning

confidence: 97%

High resolution X-ray tomography – three-dimensional characterisation of cell–scaffold constructs for cartilage tissue engineering

Zehbe¹,

Schmitt

Kirkpatrick

et al. 2015

Materials Science and Technology

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Synchrotron radiation based microcomputed tomography (SR-mCT) has become a valuable tool for the structural analysis of different types of biomaterials. This methodology allows the nondestructive investigation of specimens in their three-dimensional context. In the present paper, articular cartilage is taken as an exemplary tissue to demonstrate the suitability of the SR-mCT method for the investigation of biomaterials for different tissue engineering approaches. Thus, a biodegradable scaffold for cartilage tissue engineering in different modifications was analysed. Using enhanced phase contrast imaging, it was possible to demonstrate single cells without further metal staining. The three-dimensional data acquired for each investigated sample allowed qualitative and quantitative analyses without irreversibly damaging the samples. The use of the phase contrast mode enables the analysis of single cells within a scaffold material even under mechanical stimulation. This opens up innovative perspectives for the future study of the behaviour of cells in their three-dimensional environment and the non-destructive study of morphogenesis in cell-scaffold constructs.

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“…3A). 48,49 Small-angle X-ray scatter images can also be used to identify these three zones. 48 In addition to differentiating between different cartilage regions, PC images display the interface between calcified cartilage and bone and provide detail into tendons and ligaments that are not visible in absorption images (Fig.…”

Section: Orthopedic Tissuesmentioning

confidence: 99%

“…The volume of a single cell could be used to estimate cell density within a region of articular cartilage from CT images. 49 Cells have also been imaged after culture on polymeric scaffolds. Edge enhancement resulting from propagation-based PC imaging allowed identification of individual fibroblasts and calvarial cells on the surface of poly(ethylene terephthalate) multifilament yarns.…”

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confidence: 99%