Three-Dimensional Vascularized Lung Cancer-on-a-Chip with Lung Extracellular Matrix Hydrogels for In Vitro Screening

Park, Sang Un; Kim, Tae Hee; Kim, Soo Hyun; You, Seungkwon; Jung, Youngmee

doi:10.3390/cancers13163930

Cited by 39 publications

(24 citation statements)

References 54 publications

(66 reference statements)

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“…Consequently, VFP can provide an easier analysis of the influence of hemodynamic parameters on the course of angiogenesis in vitro. In contrast to microneedles, VFP enables a reconstruction not only of linear but also of curved and branched vascular geometries [ 31 , 38 ]. Furthermore, VFP is a highly repetitive, fast, simple, and inexpensive technique.…”

Section: Discussionmentioning

confidence: 99%

Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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Angiogenesis is the development of new blood vessels from the existing vasculature. Its malfunction leads to the development of cancers and cardiovascular diseases qualified by the WHO as a leading cause of death worldwide. A better understanding of mechanisms regulating physiological and pathological angiogenesis will potentially contribute to developing more effective treatments for those urgent issues. Therefore, the main goal of the following study was to design and manufacture an angiogenesis-on-a-chip microplatform, including cylindrical microvessels created by Viscous Finger Patterning (VFP) technique and seeded with HUVECs. While optimizing the VFP procedure, we have observed that lumen’s diameter decreases with a diminution of the droplet’s volume. The influence of Vascular Endothelial Growth Factor (VEGF) with a concentration of 5, 25, 50, and 100 ng/mL on the migration of HUVECs was assessed. VEGF’s solution with concentrations varying from 5 to 50 ng/mL reveals high angiogenic potential. The spatial arrangement of cells and their morphology were visualized by fluorescence and confocal microscopy. Migration of HUVECs toward loaded angiogenic stimuli has been initiated after overnight incubation. This research is the basis for developing more complex vascularized multi-organ-on-a-chip microsystems that could potentially be used for drug screening.

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Section: Discussionmentioning

confidence: 99%

Exploring Endothelial Expansion on a Chip

Konopka

Flont

Żuchowska

et al. 2022

Sensors

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“…The presence of vascular like architectures also enabled a comprehensive screening of anti‐angiogenesis drugs. [ 86 ] However, studies exploring lung tumor organoids generation in lung‐tumor specific dECM and in a fully vascularized setting is yet to be fully demonstrated, evidences obtained from these reports provide interesting insights into the potential of using such matrix and vascularized set‐ups for screening anti‐cancer therapeutics targeted to this neoplasia.…”

Section: Tumor Organoids—relevance In Human Neoplasia Modelling and T...mentioning

confidence: 99%

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Moura

Monteiro

Ferreira

et al. 2022

Adv Funct Materials

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The extracellular matrix plays a critical role in bioinstructing cellular self‐assembly and spatial (re)configuration processes that culminate in human organoids in vitro generation and maturation. Considering the importance of the supporting matrix, herein is showcased the most recent advances in the bioengineering of decellularized tissue hydrogels for generating organoids and assembloids. Key design blueprints, characterization methodologies, and extracellular matrix processing toolboxes are comprehensively discussed in light of current advances. Such enabling approaches provide the grounds for engineering next‐generation tissue‐specific hydrogels with close‐to‐native biomolecular signatures and user‐tailored biophysical properties that may potentiate organoids physiomimetic potential. In a forward looking perspective, the combination of tissue‐specific decellularized hydrogels with increasingly complex multicellular assemblies and bottom‐up cell engineering technologies may unravel unprecedented tissue‐like physiological responses and further advance the exploitation of organoids and assembloids as human disease surrogates or as patient‐tailored living therapeutics.

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“…Park et al used A549, HU-VECs, and human lung fibroblast cell lines to generate spheroids established on hydrogel with vascular-mimicking channels. This study presents a better in vitro model for drugdose testing [102]. Ferreira et al developed 3D microspheres from A549, fibroblasts, and bone marrow derived mesenchymal stem cell on hyaluronic microparticles.…”

Section: Hydrogel Based Technicsmentioning

confidence: 99%

Clinical Application Perspectives of Lung Cancers 3D Tumor Microenvironment Models for In Vitro Cultures

Wieleba

Wojas‐Krawczyk

Krawczyk

et al. 2022

IJMS

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Despite the enormous progress and development of modern therapies, lung cancer remains one of the most common causes of death among men and women. The key element in the development of new anti-cancer drugs is proper planning of the preclinical research phase. The most adequate basic research exemplary for cancer study are 3D tumor microenvironment in vitro models, which allow us to avoid the use of animal models and ensure replicable culture condition. However, the question tormenting the scientist is how to choose the best tool for tumor microenvironment research, especially for extremely heterogenous lung cancer cases. In the presented review we are focused to explain the key factors of lung cancer biology, its microenvironment, and clinical gaps related to different therapies. The review summarized the most important strategies for in vitro culture models mimicking the tumor–tumor microenvironmental interaction, as well as all advantages and disadvantages were depicted. This knowledge could facilitate the right decision to designate proper pre-clinical in vitro study, based on available analytical tools and technical capabilities, to obtain more reliable and personalized results for faster introduction them into the future clinical trials.

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Three-Dimensional Vascularized Lung Cancer-on-a-Chip with Lung Extracellular Matrix Hydrogels for In Vitro Screening

Cited by 39 publications

References 54 publications

Exploring Endothelial Expansion on a Chip

Exploring Endothelial Expansion on a Chip

Advancing Tissue Decellularized Hydrogels for Engineering Human Organoids

Clinical Application Perspectives of Lung Cancers 3D Tumor Microenvironment Models for In Vitro Cultures

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