1995
DOI: 10.1083/jcb.129.6.1601
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Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle.

Abstract: Abstract. The three dimensional organization of microtubules in mitotic spindles of the yeast Saccharomyces cerevisiae has been determined by computer-aided reconstruction from electron micrographs of serially cross-sectioned spindles. Fifteen spindles ranging in length from 0.6-9.4 Ixm have been analyzed. Ordered microtubule packing is absent in spindles up to 0.8 Ixm, but the total number of microtubules is sufficient to allow one microtubule per kinetochore with a few additional microtubules that may form a… Show more

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Cited by 434 publications
(537 citation statements)
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“…The median MT length in cells during late anaphase decreases sharply to only ϳ30 nm (n ϭ 12) or just slightly longer than an MT's width (also see Figure 7C, right). These very short MTs lie at the face of the SPB; they are invisible in standard thin sections (ϳ50 -70 nm) and thus were not detected in our previous study of yeast spindle structure (Winey et al, 1995). Very short MTs lying at the SPB face confirm that anaphase A occurs in this organism.…”
Section: The 3-d Geometry Of Early Mitotic Spindle Formation In S Cesupporting
confidence: 46%
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“…The median MT length in cells during late anaphase decreases sharply to only ϳ30 nm (n ϭ 12) or just slightly longer than an MT's width (also see Figure 7C, right). These very short MTs lie at the face of the SPB; they are invisible in standard thin sections (ϳ50 -70 nm) and thus were not detected in our previous study of yeast spindle structure (Winey et al, 1995). Very short MTs lying at the SPB face confirm that anaphase A occurs in this organism.…”
Section: The 3-d Geometry Of Early Mitotic Spindle Formation In S Cesupporting
confidence: 46%
“…Our previous work on spindle structure in budding yeast used reconstructions from serial thin sections to document the 3-D geometry of bipolar spindles, but we were not able to track the MTs growing from side-by-side SPBs (Winey et al, 1995). The tomographic reconstruction method used here, however, allows one to section the same volume in multiple orientations, which has let us characterize the 3-D geometry of nuclear MT arrays in interphase cells and in forming mitotic spindles, including MT arrays from putative old and new SPBs.…”
Section: Structural Organization Of Forming Mitotic Spindlesmentioning
confidence: 99%
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