Three-Dimensional Tumor Model and T-Lymphocytes Immunotherapy for Cancer

Liu, Hua

doi:10.1016/b978-0-12-397833-2.00011-x

Cited by 1 publication

(1 citation statement)

References 74 publications

(113 reference statements)

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“…It is known that immune response activation helps in the complete eradication of tumor cells [16]. However, conventional ECT does not induce enough stimulation of the immune system to prevent the formation of new tumors or the growth of distant tumors.…”

Section: Introductionmentioning

confidence: 99%

A Novel Method for Controlled Gene Expression via Combined Bleomycin and Plasmid DNA Electrotransfer

Chopra¹,

Ruzgys²,

Jakutavičiūtė³

et al. 2019

IJMS

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Electrochemotherapy is an efficient method for the local treatment of cutaneous and subcutaneous metastases, but its efficacy as a systemic treatment remains low. The application of gene electrotransfer (GET) to transfer DNA coding for immune system modulating molecules could allow for a systemic effect, but its applications are limited because of possible side effects, e.g., immune system overactivation and autoimmune response. In this paper, we present the simultaneous electrotransfer of bleomycin and plasmid DNA as a method to increase the systemic effect of bleomycin-based electrochemotherapy. With appropriately selected concentrations of bleomycin and plasmid DNA, it is possible to achieve efficient cell transfection while killing cells via the cytotoxic effect of bleomycin at later time points. We also show the dynamics of both cell electrotransfection and cell death after the simultaneous electrotransfer of bleomycin and plasmid DNA. Therefore, this method could have applications in achieving the transient, cell death-controlled expression of immune system activating genes while retaining efficient bleomycin mediated cell killing.

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Section: Introductionmentioning

confidence: 99%