Three-dimensional transfer functions for high-aperture systems

Sheppard, Colin J. R.; Gu, Miṅ; Kawata, Yoshimasa; Kawata, Satoshi

doi:10.1364/josaa.11.000593

Cited by 107 publications

(71 citation statements)

References 9 publications

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“…To analyze both lateral resolution and depth (z) sectioning theoretically, we use the 3D CTF of the imaging system [53][54][55][56][57], with the caveat that this theory assumes a single-or weak scattering approximation (e.g., Born or Rytov model) [58]. Analytical theory for multiple scattering is not available, but many procedures start from single scattering and apply it recursively [58], so this should provide a good starting point for resolution analysis.…”

Section: Analysis Of Resolutionmentioning

confidence: 99%

3D intensity and phase imaging from light field measurements in an LED array microscope

Tian¹,

Waller²

2015

Optica

397

289

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Section: Analysis Of Resolutionmentioning

confidence: 99%

3D intensity and phase imaging from light field measurements in an LED array microscope

Tian¹,

Waller²

2015

Optica

397

289

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“…There are several methods to theoretically model the recording system and thus model the PSF, e.g. [29,6,1] or its Fourier domain equivalent, the optical transfer function (OTF) [27,21,24]. A theoretical PSF according to [6] computed with [16] is depicted in figure 2(b).…”

Section: Physical Modelmentioning

confidence: 99%

Blind Deconvolution of Widefield Fluorescence Microscopic Data by Regularization of the Optical Transfer Function (OTF)

Keuper

Schmidt

Temerinac-Ott

et al. 2013

2013 IEEE Conference on Computer Vision and Pattern Recognition

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With volumetric data from widefield fluorescence microscopy, many emerging questions in biological and biomedical research are being investigated. Data can be recorded with high temporal resolution while the specimen is only exposed to a low amount of phototoxicity. These advantages come at the cost of strong recording blur caused by the infinitely extended point spread function (PSF). For widefield microscopy, its magnitude only decays with the square of the distance to the focal point and consists of an airy bessel pattern which is intricate to describe in the spatial domain. However, the Fourier transform of the incoherent PSF (denoted as Optical Transfer Function (OTF)) is well localized and smooth. In this paper, we present a blind deconvolution method that improves results of state-of-theart deconvolution methods on widefield data by exploiting the properties of the widefield OTF.

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“…The Fourier space equivalent, the OTF, also has a long history [7,13,20]. However, the CPM defined in (4) is an unusual microscope element:…”

Section: Wavefront Coding Theorymentioning

confidence: 99%

“…For full investigation of the spatial frequency response of a high aperture microscope, we would normally look to the 3D OTF [7,13,19,20]. We have recently published a method for calculating the 3D OTF suitable for arbitrary pupil filters [1] which can be applied directly to find the OTF for a cubic phase plate.…”

Section: High Aperture Otf Modelmentioning

confidence: 99%