Three-dimensional structure of the complexes between bovine chymotrypsinogen A and two recombinant variants of human pancreatic secretory trypsin inhibitor (Kazal-type)

Predicting the reactivity of proteins from their sequence alone: Kazal family of protein inhibitors of serine proteinases

“…The hydrogen bond shortens on complex formation with SGPB (29) and CHYM (30) but not with HLE (31). The same shortening is seen when the structure of variant 3 of PSTI (38) (this variant has P 2 Thr-17 and P 1 Ј Glu-19) is compared in free inhibitor and in complex with chymotrypsinogen (34). A few tests based on ⌬⌬G°v alue indicate major P 2 -P 1 Ј pair nonadditivity.…”

“…X-ray crystallography of complexes of OMTKY3, of many of its variants, and of some PSTI variants with SGPB, CHYM and HLE showed that a consensus set of 12 contact positions in the inhibitors is in contact with the enzymes (29)(30)(31)(32)(33)(34) (Fig. 2).…”

Modelling protein docking using shape complementarity, electrostatics and biochemical information 1 1Edited by J. Thornton

“…The PDB code of the complex is 1cgi (Hecht et al, 1991). (2) CHO, ovomucoid (2ovo) (Bode et al, 1985) bound to a chymotrypsin (5cha) (Blevins & Tulinsky, 1985).…”

Crystal Structure of Human Trypsin 1: Unexpected Phosphorylation of Tyr151

“…The available X-ray structures of complexes between a SPTI an a serine (pro-) protease are the following; porcine SPTI and trypsinogen (Bolognesi et al, 1982; PDB code 1tgs); human variant 3 SPTI (humv3) and chymotrypsinogen (Hecht et al, 1991;PDB code 1cgi). Footprints of inhibitor binding as observed in these complexes are shown in Figure 11.…”