Three-Dimensional Skin Models of Psoriasis

Соболева, А. Г.; Mezentsev, Alexandre; Zolotorenko, Alena; Брускин, С. А.; Pirusian, Eleonora

doi:10.1159/000369925

Cited by 7 publications

(7 citation statements)

References 61 publications

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“…Few hurdles are, however, hindering immune cell incorporated models, including the uniform application of the cells, their migration in the ECM, and the medium requirements for co-culture. 64 The first model of HSE populated with immune cells was presented by van den Bogaard et al 97 Briefly, allogeneic CD4 þ T cells were stimulated using anti-CD3/CD28 mAb-coated beads and were seeded underneath fully developed DED skin equivalents. After two days, activated CD4 þ T cells migrated towards the epidermis and pro-inflammatory cytokine and chemokine production was observed, including IL-6, IL-8, IL-23 and CXCL10.…”

Section: Development Of Ft Skin Equivalentsmentioning

confidence: 99%

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

Desmet

Ramadhas

Lambert

et al. 2017

Exp Biol Med (Maywood)

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Psoriasis is a complex chronic immune-mediated inflammatory cutaneous disease associated with the development of inflammatory plaques on the skin. Studies proved that the disease results from a deregulated interplay between skin keratinocytes, immune cells and the environment leading to a persisting inflammatory process modulated by pro-inflammatory cytokines and activation of T cells. However, a major hindrance to study the pathogenesis of psoriasis more in depth and subsequent development of novel therapies is the lack of suitable pre-clinical models mimicking the complex phenotype of this skin disorder. Recent advances in and optimization of three-dimensional skin equivalent models have made them attractive and promising alternatives to the simplistic monolayer cultures, immunological different in vivo models and scarce ex vivo skin explants. Moreover, human skin equivalents are increasing in complexity level to match human biology as closely as possible. Here, we critically review the different types of three-dimensional skin models of psoriasis with relevance to their application potential and advantages over other models. This will guide researchers in choosing the most suitable psoriasis skin model for therapeutic drug testing (including gene therapy via siRNA molecules), or to examine biological features contributing to the pathology of psoriasis. However, the addition of T cells (as recently applied to a de-epidermized dermis-based psoriatic skin model) or other immune cells would make them even more attractive models and broaden their application potential. Eventually, the ultimate goal would be to substitute animal models by three-dimensional psoriatic skin models in the pre-clinical phases of anti-psoriasis candidate drugs. Impact statement The continuous development of novel in vitro models mimicking the psoriasis phenotype is important in the field of psoriasis research, as currently no model exists that completely matches the in vivo psoriasis skin or the disease pathology. This work provides a complete overview of the different available in vitro psoriasis models and suggests improvements for future models. Moreover, a focus was given to psoriatic skin equivalent models, as they offer several advantages over the other models, including commercial availability and validity. The potential and reported applicability of these models in psoriasis pre-clinical research is extensively discussed. As such, this work offers a guide to researchers in their choice of pre-clinical psoriasis model depending on their type of research question.

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Section: Development Of Ft Skin Equivalentsmentioning

confidence: 99%

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

Desmet

Ramadhas

Lambert

et al. 2017

Exp Biol Med (Maywood)

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“…Simple psoriasis models, which are also commercially available (MatTek Corp., Ashland, MA, USA), are composed of healthy keratinocytes and diseased fibroblasts isolated from psoriatic lesions of patients. However, the use of psoriatic epidermal keratinocytes more closely resembles psoriatic conditions [41].…”

Section: Hydrogel Systemsmentioning

confidence: 99%

“…Prospectively, iPSCs have the potential to predict patient-specific response to therapies. This is of particular importance since current treatments for psoriasis and other skin disorders are not effective in all patients [41]. Furthermore, it is an unlimited source of cells.…”

Section: Outlook: Skin Organoids and Their Potential Use For Personalmentioning

confidence: 99%

In vitro skin three-dimensional models and their applications

Klicks

Molitor

Ertongur-Fauth

et al. 2017

JCB

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Abstract. Skin fulfils a plethora of eminent physiological functions ranging from physical barrier over immunity shield to the interface mediating social interaction. Prone to several acquired and inherited diseases, skin is therefore a major target of pharmaceutical and cosmetic research. The lack of similarity between human and animal skin and rising ethical concerns in the use of animal models have driven the search for novel realistic three-dimensional skin models. This review provides a survey of contemporary skin models and compares them in terms of applicability, reliability, cost and complexity.Keywords: Skin, phenotypic screening, 3D models, pharmaceutical research Skin -composition and functionHuman skin covers an area of almost 2 m 2 in the adult and consists of the three major layers, subcutis, dermis, and epidermis. The subcutis is composed of adipose and epithelial cells. It harbours blood vessels, neurites of peripheral neurons, Vater-Pacini mechanosensors, and, partially, also sweat glands and hair follicles. It connects the skin to periosteum and fascia, absorbs forces, and mediates thermal insulation. The dermis supplies the epidermis with mechanical support and nutrients. It is stratified into an inner, reticular, and an outer, papillary, zone. The dermis houses most sebaceous glands, sweat glands, hair follicles, smooth muscle cells, and capillary beds and, thus, regulates skin moisture, body temperature, and performs the secretory function of skin. The papillary layer is characterized by relatively loose connective tissue, where Meissner corpuscles sense touch. Immune cells, particularly mast cells and dendritic cells, are patrolling in the papillary layer and mediate local inflammatory reactions and immune surveillance. Finally, dermal fibroblasts secrete extracellular matrix (ECM) and basement membrane components. These are primarily collagens I and III, and a proteoglycan-rich ground substance [1]. The resulting ECM mediates tensile strength of the dermis. The dermo-epidermal junction is centered around a special basement membrane. This is composed of a laminin/collagen IV scaffold and further typical basement membrane components such as perlecans and nidogens [1].The epidermis is a squamous epithelium of 50-100 m thickness. It is devoid of blood vessels but contains keratinocytes, Merkel cell mechanosensors, Langerhans immune cells, and melanocytes. The 1 These authors contributed equally.

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“…The purpose of tissue engineering is the creation of three-dimensional (3D) tissue constructs that will benefit human health [ 1 , 2 ]. To date, the tissue constructs of quite thin [ 3 , 4 , 5 , 6 ] and/or those composed of the cells with a low oxygen demand [ 7 , 8 , 9 , 10 ] have been successfully fabricated. The fabrication of dense tissues faces problems in developing methods for constructing perfusable vascular-like network inside them [11] .…”

Section: Introductionmentioning

confidence: 99%

Engineering tissues with a perfusable vessel-like network using endothelialized alginate hydrogel fiber and spheroid-enclosing microcapsules

Liu

Sakai

Taya

2016

Heliyon

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Development of the technique for constructing an internal perfusable vascular network is a challenging issue in fabrication of dense three-dimensional tissues in vitro. Here, we report a method for realizing it. We assembled small tissue (about 200 μm in diameter)-enclosing hydrogel microcapsules and a single hydrogel fiber, both covered with human vascular endothelial cells in a collagen gel. The microcapsules and fiber were made from alginate and gelatin derivatives, and had cell adhesive surfaces. The endothelial cells on the hydrogel constructs sprouted and spontaneously formed a network connecting the hydrogel constructs with each other in the collagen gel. Perfusable vascular network-like structure formation after degrading the alginate-based hydrogel constructs by alginate lyase was confirmed by introducing solution containing tracer particles of about 3 μm in diameter into the lumen templated by the alginate hydrogel fiber. The introduced solution flowed into the spontaneously formed capillary branches and passed around the individual spherical tissues.

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Three-Dimensional Skin Models of Psoriasis

Cited by 7 publications

References 61 publications

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

In vitro psoriasis models with focus on reconstructed skin models as promising tools in psoriasis research

In vitro skin three-dimensional models and their applications

Engineering tissues with a perfusable vessel-like network using endothelialized alginate hydrogel fiber and spheroid-enclosing microcapsules

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