Three-dimensional silk impregnated HAp/PHBV nanofibrous scaffolds for bone regeneration

Güneş, Oylum Çolpankan; Ünalan, Irem; Çeçen, Berivan; Albayrak, Aylin Ziylan; Havıtçıoğlu, Hasan; Ustun, Ozcan; Ergur, Bekir Uğur

doi:10.1080/00914037.2018.1443928

Cited by 15 publications

(6 citation statements)

References 43 publications

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“…18,19 Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a widely used copolymer for tissue engineering scaffolds due to its biocompatible and biodegradable properties as well as its good spinnability in wet-electrospinning. 20…”

Section: Introductionmentioning

confidence: 99%

Wet-electrospun PHBV nanofiber reinforced carboxymethyl chitosan-silk hydrogel composite scaffolds for articular cartilage repair

et al. 2020

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The objective of the study was to produce three-dimensional and porous nanofiber reinforced hydrogel scaffolds that can mimic the hydrated composite structure of the cartilage extracellular matrix. In this regard, wet-electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofiber reinforced carboxymethyl chitosan-silk fibroin (PNFs/CMCht-SF) hydrogel composite scaffolds that were chemically cross-linked by poly(ethylene glycol) diglycidyl ether (PEGDE) were produced. To the best of our knowledge, this is the first study in cartilage regeneration where a three dimensional porous spongy composite scaffold was obtained by the dispersion of wet-electrospun nanofibers within a polymer matrix. All of the produced hydrogel composite scaffolds had an interconnected microporous structure with well-integrated PHBV nanofibers on the pore walls. The scaffold comprising an equal amount of PEGDE and polymer (PNFs/CMCht-SF1:PEGDE1) demonstrated comparable water content (91.4 ± 0.7%), tan δ (0.183 at 1 Hz) and compressive strength (457 ± 85 kPa) values to that of articular cartilage. Besides, based on the histological analysis, this hydrogel composite scaffold supported the chondrogenic differentiation of bone marrow mesenchymal stem cells. Consequently, this hydrogel composite scaffold presented a great promise for cartilage tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Wet-electrospun PHBV nanofiber reinforced carboxymethyl chitosan-silk hydrogel composite scaffolds for articular cartilage repair

et al. 2020

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“…Bone tissue engineering aim is to repair or replace lost large bone tissues caused by trauma or disease. By the use of scaffolds with the properties, porous, biocompatible, bioactive, biodegradable, high mechanical strength, sufficient to make it structurally stable for the formation of new tissue, and mimic the bone extracellular matrix (ECM), that provides the microenvironment for cell attachment, also, the use of bioactive agents promote cell migration, proliferation, differentiation, diffusion of oxygen and nutrients, this together produces new tissue formation and thus bone regeneration [56]- [58]. Bone scaffolds are better if their pores are distributed between 10-400 µm, with microscale and macroscale pores [56].…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

“…2019, prepared by wet electrospinning 3D scaffold of silk fibroin (0.5 % (w/v)) with poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) (Mw: 80.000 Da) (3 % (w/v)) and benzyl triethylammonium chloride (BTEAC) (0.2 % (w/v)) in chloroform as solvent, with hydroxyapatite (HAp) 0.3 % (w/v), ethanol-water (9:1 v: v) was used to collect the nanofibers. In vitro cell culture tests using MG-63 osteosarcoma human cells revealed improved biomineralization, cell viability, alkaline phosphatase (ALP) activity after 10 days of cell culture, the porous and interconnected nanofibers aided the growth and distribution of osteoblasts within the scaffolds [58].…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Wet Electrospinning and its Applications: A Review

Suaza

Henao

2022

TecnoL.

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In wet electrospinning, a natural or synthetic polymer solution is deposited on a non-solvent liquid coagulant used as collector. This technique can create 3D nanofiber scaffolds with better properties (e.g., porosity and high surface area) than those of traditional 2D scaffolds produced by standard electrospinning. Thanks to these characteristics, wet electrospinning can be employed in a wide range of tissue engineering and industrial applications. This review aims to broaden the panorama of this technique, its possible fields of action, and its range of common materials. Moreover, we also discuss its future trends. In this study, we review papers on this method published between 2017 and 2021 to establish the state of the art of wet electrospinning and its most important applications in cardiac, cartilage, hepatic, wound dressing, skin, neural, bone, and skeletal muscle tissue engineering. Additionally, we examine its industrial applications in water purification, air filters, energy, biomedical sensors, and textiles. The main results of this review indicate that 3D scaffolds for tissue engineering applications are biocompatible; mimic the extracellular matrix (ECM); allow stem cell viability and differentiation; and have high porosity, which provides greater cell infiltration compared to 2D scaffolds. Finally, we found that, in industrial applications of wet electrospinning: (1) additives improve the performance of pure polymers; (2) the concentration of the solution influences porosity and fiber packing; (3) flow rate, voltage, and distance modify fiber morphology; (4) the surface tension of the non-solvent coagulant on which the fibers are deposited has an effect on their porosity, compaction, and mechanical properties; and (5) deposition time defines scaffold thickness.

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“…Highly porous, 3D scaffolds can be obtained via an alternative electrospinning approach: using a coagulation bath of non-solvent as the collector. [22][23][24][25][26][27][28][29][30][31][32][33][34][35] However, while these studies have yielded 3D scaffolds composed of fibers that are not straight, the random fiber morphology is very far from the desired coiled, hierarchal morphology. Recently, work by Taskin et al 36 described electrospinning of polycaprolactone (PCL) into an ethanol coagulation bath to obtain highly porous 3D scaffolds composed of coiled fibers, which facilitated myofibroblast differentiation and contraction.…”

Section: Introductionmentioning

confidence: 99%

Architectured helically coiled scaffolds from elastomeric poly(butylene succinate) (PBS) copolyester via wet electrospinning

Sonseca¹,

Sahay²,

Stępień³

et al. 2019

Preprint

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<div><div><div><p>Electrospinning is one of the most investigated methods used to produce polymeric fiber structures that mimic the morphology of native extracellular matrix. These structures have been extensively studied in the context of scaffolds for tissue regeneration. However, the compactness of materials obtained by traditional electrospinning, collected as two-dimensional non-woven scaffolds, can limit cell infiltration and tissue ingrowth. In addition, for applications in smooth muscle tissue engineering, highly elastic scaffolds capable of withstanding cyclic mechanical strains without suffering significant permanent deformations are preferred. In order to address these challenges, we report the fabrication of microscale 3D helically coiled structures (referred as 3D-HCS) by wet-electrospinning method, a modification of the traditional electrospinning process in which a coagulation bath (non-solvent system for the electrospun material) is used as the collector. The present study, for the first time, successfully demonstrates the feasibility of using this method to produce various architectures of 3D-HCS from segmented copolyester of poly(butylene succinate-co- dilinoleic succinate) (PBS-DLS), a thermoplastic elastomer. A mechanism for the HCS formation is proposed and verified with experimental data. Fabricated 3D-HCS showed high specific surface area, high porosity, and good elasticity. Further, the marked increase in cell proliferation on 3D-HCS confirmed the suitability of these materials as scaffolds for soft tissue engineering.</p></div></div></div>

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Three-dimensional silk impregnated HAp/PHBV nanofibrous scaffolds for bone regeneration

Cited by 15 publications

References 43 publications

Wet-electrospun PHBV nanofiber reinforced carboxymethyl chitosan-silk hydrogel composite scaffolds for articular cartilage repair

Wet-electrospun PHBV nanofiber reinforced carboxymethyl chitosan-silk hydrogel composite scaffolds for articular cartilage repair

Wet Electrospinning and its Applications: A Review

Architectured helically coiled scaffolds from elastomeric poly(butylene succinate) (PBS) copolyester via wet electrospinning

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