Three-dimensional silk fibroin microsphere-nanofiber scaffolds for vascular tissue engineering

Liu, Qiang; Ying, Guoliang; Jiang, Nan; Yetisen, Ali K.; Yao, Danyu; Xie, Xiao-ying; Fan, Yubo; Liu, Haifeng

doi:10.1016/j.medntd.2020.100051

Cited by 15 publications

(16 citation statements)

References 46 publications

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“… 280 Moreover, a combination of electrospinning and microfluidics has been used to produce layer by layer pure silk nanofibers and their microdroplets to sustain endothelial cell monolayers and prevent thrombus formation. 281 …”

Section: Electrospun Bm Mimicsmentioning

confidence: 99%

Mimicking the Natural Basement Membrane for Advanced Tissue Engineering

et al. 2022

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Advancements in the field of tissue engineering have led to the elucidation of physical and chemical characteristics of physiological basement membranes (BM) as specialized forms of the extracellular matrix. Efforts to recapitulate the intricate structure and biological composition of the BM have encountered various advancements due to its impact on cell fate, function, and regulation. More attention has been paid to synthesizing biocompatible and biofunctional fibrillar scaffolds that closely mimic the natural BM. Specific modifications in biomimetic BM have paved the way for the development of in vitro models like alveolar-capillary barrier, airway models, skin, blood-brain barrier, kidney barrier, and metastatic models, which can be used for personalized drug screening, understanding physiological and pathological pathways, and tissue implants. In this Review, we focus on the structure, composition, and functions of in vivo BM and the ongoing efforts to mimic it synthetically. Light has been shed on the advantages and limitations of various forms of biomimetic BM scaffolds including porous polymeric membranes, hydrogels, and electrospun membranes This Review further elaborates and justifies the significance of BM mimics in tissue engineering, in particular in the development of in vitro organ model systems.

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Section: Electrospun Bm Mimicsmentioning

confidence: 99%

Mimicking the Natural Basement Membrane for Advanced Tissue Engineering

et al. 2022

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“…Particularly, 10%CS/Gt/PCL nanofi bers display favorable cell attachment, elongation, and proliferation. [55] Silk fi broin (SF) nanofi bers and homogeneous microspheres Vascular tissue engineering that cells cultured in 3D SF microsphere-nanofi ber scaff olds conferred the advantage of a matrix milieu that maintained cell-cell and cell-biomaterial interactions, which might also lead to the adherent cell networks and further transited to a potential in vivo microenvironment [56] Chitosan/alginate nanofi bers encapsulated by hydroxyapatite (HAp) and collagen…”

Section: Tissue Engineeringmentioning

confidence: 99%

“…2. Preparation and images of SF nanofi ber, 3D microsphere-nanofi ber scaff olds and tubular scaff olds [56].…”

Section: Wound Dressingsmentioning

confidence: 99%

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Biomedical Applications of Nanofibers

2007

Science and Technology of Polymer Nanofibers

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The idea of creating replacement for damaged or diseased tissue, which will mimic the physiological conditions and simultaneously promote regeneration by patients' own cells, has been a major challenge in the biomedicine for more than a decade. Therefore, nanofi bers are a promising solution to address these challenges. Nanofi ber technology is an exciting area attracting the attention of many researchers as a potential solution to these current challenges in the biomedical fi eld such as burn and wound care, organ repair, and treatment for osteoporosis and various diseases. Nanofi bers mimic the porous topography of natural extracellular matrix (ECM), hence they are advantageous for tissue regeneration . In biomedical engineering, electrospinning exhibits advantages as a tissue engineering scaff olds producer, which can make appropriate resemblance in physical structure with ECM. This is because of the nanometer scale of ECM fi brils in diameter, which can be mimicked by electrospinning procedure as well as its porous structure. In this review, the applications of nanofi bers in various biomedical areas such as tissue engineering, wound dressing and facemask, are summarized. It provides opportunities to develop new materials and techniques that improve the ability for developing quick, sensitive and reliable analytical techniques.

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“…The electrospinning technique is a process that uses electrostatic force to obtain nanofiber arrays from natural or synthetic polymers, resembling the extracellular matrix, with the advantage to control several morphologic characteristics during scaffold production, rendering more optimal conditions for cell integration, proliferation, migration and/or differentiation [15][16][17][18][19][20]. However, low cellular adaptation to the biomaterial scaffold constitutes a frequent limitation; hence, it is necessary to optimize biomaterials into constructs eliciting cellular attachment, integration and eventual accomplishment of physiological requirements of the organism [21,22].…”

Section: Introductionmentioning

confidence: 99%

Electrospinning-Generated Nanofiber Scaffolds Suitable for Integration of Primary Human Circulating Endothelial Progenitor Cells

et al. 2022

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The extracellular matrix is fundamental in order to maintain normal function in many organs such as the blood vessels, heart, liver, or bones. When organs fail or experience injury, tissue engineering and regenerative medicine elicit the production of constructs resembling the native extracellular matrix, supporting organ restoration and function. In this regard, is it possible to optimize structural characteristics of nanofiber scaffolds obtained by the electrospinning technique? This study aimed to produce partially degraded collagen (gelatin) nanofiber scaffolds, using the electrospinning technique, with optimized parameters rendering different morphological characteristics of nanofibers, as well as assessing whether the resulting scaffolds are suitable to integrate primary human endothelial progenitor cells, obtained from peripheral blood with further in vitro cell expansion. After different assay conditions, the best nanofiber morphology was obtained with the following electrospinning parameters: 15 kV, 0.06 mL/h, 1000 rpm and 12 cm needle-to-collector distance, yielding an average nanofiber thickness of 333 ± 130 nm. Nanofiber scaffolds rendered through such electrospinning conditions were suitable for the integration and proliferation of human endothelial progenitor cells.

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Three-dimensional silk fibroin microsphere-nanofiber scaffolds for vascular tissue engineering

Cited by 15 publications

References 46 publications

Mimicking the Natural Basement Membrane for Advanced Tissue Engineering

Mimicking the Natural Basement Membrane for Advanced Tissue Engineering

Biomedical Applications of Nanofibers

Electrospinning-Generated Nanofiber Scaffolds Suitable for Integration of Primary Human Circulating Endothelial Progenitor Cells

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