Three‐dimensional rendering of otolith growth using phase contrast synchrotron tomography

Abstract

Abstract:We present a three-dimensional computer reconstruction of a plaice (Pleuronectes platessa) otolith from data acquired by the Diamond Light synchrotron, beamline I12, X-ray source, a high energy (53 -150 keV) source particularly well suited to the study of dense objects. Our data allowed non-destructive rendering of otolith structure, and for the first time allows examination of otolith annuli (internal ring structures) to be analysed in X-ray tomographic images. If 'Yes', provide details. Normally these procedures will be considered unacceptable by JFB. Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation Ethics questionnaire for JFBIf 'No', did any of the procedures, particularly those that involve lethal endpoints, cause adverse effects or lasting harm to a sentient animal?If 'Yes', provide details. Normally these procedures will be considered unacceptable by JFB unless any harm caused can be justified against the benefit gained. We present a three-dimensional computer reconstruction of a plaice (Pleuronectes platessa) 16

Otolith development in wild populations of stickleback: Jones & Hynes method does not apply to most populations

“…The determination of age in fishes by counting of annual rings on otoliths has been well established among researchers and fisheries managers for decades (Campana, ; Jones & Hynes, ). The method is still widely used (Koeda et al ., ; Robertson et al ., ; Uriarte et al ., ) though more recent approaches for otolith analysis, such as daily increment quantification, microchemistry, carbon dating, plasma mass spectrometry and three dimensional x‐ray scanning (Hippel et al ., ; Jones & Chen, ; Mapp et al ., ; Thorrold & Shuttleworth, ) are more powerful for age estimation. Reading of otolith annual rings, if validated, is still useful in ecological studies because it is cheap and more easily applied to large samples.…”

Peeling the Otolith of Fish: Optimal Parameterization for Micro-CT Scanning

“…Yan et al (2009) found an HU between 2,500 and 4,600 for asteriscus and lapillus of the carp otolith, respectively. Mapp et al (2016) and FAbOSA group (2002) did not present HU values. Nevertheless, other calcified structures are out of this range, e.g., scallop shells (744 HU) (Diez et al, 2013) and ureteral stone (1,350 HU) (Hameed et al, 2013).…”

“…More recently, Yan et al (2009) used a high voltage (130 kV) and low current (30 µA), where higher photon energies penetrated the otolith and no information on density was captured. Mapp et al (2016) using monochromatic X-ray with 53 kV and no filter, rendered 3D imagery highlighting the growth rings. Although those authors considered that the objective proposed was reached, they reported some artifacts and did not experiment with other tomography settings.…”

“…Browning et al (2012) also used CT to analyze morphology changes as consequence of a stressful condition in Red Drum (Sciaenops ocellatus) and Bignami et al (2013) to see how ocean acidification influence on Cobia (Rachycentron canadum) otoliths. More recently, Mapp et al (2016) used a non-conventional tomography, the synchrotron radiation, to render an otolith of Plaice (Pleuronectes platessa) and shown growth rings.…”

“…Though many of these studies (except for FAbOSA) have succeeded in their objectives, they do not present the methodological aspects behind this new experimental procedure, which may be considered a relatively costly time and expensive technique, mainly the one used by Mapp et al (2016). In the present work, we describe a protocol developed for the optimal acquisition of conventional micro-CT images of sagittal otoliths, considering different species and a spectrum of CT parameters.…”