Abstract:The profi le of the surfaces of two samples of monocrystalline silicon containing trapezoidal-shaped protrusions is established by means of three-dimensional reconstruction from stereoscopic images obtained in a scanning electron microscope and the average value of the height of the protrusions is determined. The results are compared with the results of measurements by an atomic-force microscope. The advantage in terms of precision of reconstruction for a sample that has been subjected to additional plasma tre… Show more
“…Compared with the x-y stage, the tilting stage is able to tilt the sample to a certain degree under the microscope, whereby it possible to image the sample from different orientations and to construct a 3D structure. For instance, this technique has been successfully used to observe biological cells under an optical microscope (OM) [ 15 , 16 ], and fracture surfaces with subsequent 3D reconstruction under a scanning electron microscope (SEM) [ 17 , 18 ], and nanomaterials under a transmission electron microscope (TEM) [ 19 , 20 ]. However, in this method, more than half of the sample data still cannot be obtained due to the small tilting angles possible.…”
Image sensing at a small scale is essentially important in many fields, including microsample observation, defect inspection, material characterization and so on. However, nowadays, multi-directional micro object imaging is still very challenging due to the limited field of view (FOV) of microscopes. This paper reports a novel approach for multi-directional image sensing in microscopes by developing a rotatable robot. First, a robot with endless rotation ability is designed and integrated with the microscope. Then, the micro object is aligned to the rotation axis of the robot automatically based on the proposed forward-backward alignment strategy. After that, multi-directional images of the sample can be obtained by rotating the robot within one revolution under the microscope. To demonstrate the versatility of this approach, we view various types of micro samples from multiple directions in both optical microscopy and scanning electron microscopy, and panoramic images of the samples are processed as well. The proposed method paves a new way for the microscopy image sensing, and we believe it could have significant impact in many fields, especially for sample detection, manipulation and characterization at a small scale.
“…Compared with the x-y stage, the tilting stage is able to tilt the sample to a certain degree under the microscope, whereby it possible to image the sample from different orientations and to construct a 3D structure. For instance, this technique has been successfully used to observe biological cells under an optical microscope (OM) [ 15 , 16 ], and fracture surfaces with subsequent 3D reconstruction under a scanning electron microscope (SEM) [ 17 , 18 ], and nanomaterials under a transmission electron microscope (TEM) [ 19 , 20 ]. However, in this method, more than half of the sample data still cannot be obtained due to the small tilting angles possible.…”
Image sensing at a small scale is essentially important in many fields, including microsample observation, defect inspection, material characterization and so on. However, nowadays, multi-directional micro object imaging is still very challenging due to the limited field of view (FOV) of microscopes. This paper reports a novel approach for multi-directional image sensing in microscopes by developing a rotatable robot. First, a robot with endless rotation ability is designed and integrated with the microscope. Then, the micro object is aligned to the rotation axis of the robot automatically based on the proposed forward-backward alignment strategy. After that, multi-directional images of the sample can be obtained by rotating the robot within one revolution under the microscope. To demonstrate the versatility of this approach, we view various types of micro samples from multiple directions in both optical microscopy and scanning electron microscopy, and panoramic images of the samples are processed as well. The proposed method paves a new way for the microscopy image sensing, and we believe it could have significant impact in many fields, especially for sample detection, manipulation and characterization at a small scale.
“…For instance, various title stages have been adopted to rotate samples recently. 15,16 Yet, these stages can only tilt samples within a small angle, which means that some surface defects are still invisible. To increase the title range, some manipulators and holders have been designed to rotate samples in a 360 • way.…”
Surface defect is regarded as one critical factor that affects magnetic properties of magnetic microwires. However, current imaging techniques only allow to observe samples from one fixed direction, and thereby most of surface defects on microwire cannot be detected. Herein, we firstly develop a miniature rotatable robot inside scanning electron microscopy (SEM) and propose a relevant control strategy to align the microwire onto the rotation axis of the robot. After that, the microwire is rotated continuously by 360o and all the surface defects on the microwire are observed from different directions successfully. Multidirectional observation results can be used to located heating inhomogeneity, which is the main cause of defects. Magnetic measurement results show that the effect of defects on domain wall (DW) should be considered in device design. This research provides the direct evidence for surface defects’ distribution and effect, which can be adopted to provide guidance for improving magnetic wire’s fabrication process and designing logic circuits made from those magnetic wires.
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