Three‐dimensional Reconstruction of the Equine Ovary

Kimura, Junko; Hirano, Yusuke; Takemoto, Satoko; Nambo, Yasuo; Ishinazaka, Tsuyoshi; Himeno, Ryutaro; Mishima, Taketoshi; Tsumagari, Shigehisa; Yokota, Hideo

doi:10.1111/j.1439-0264.2004.00567.x

Cited by 18 publications

(13 citation statements)

References 11 publications

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“…The CoMBI method involves 3D imaging by serial block-face imaging, which is a technique for reconstructing 3D images from a series of block-face images. Some laboratories have developed apparatus for obtaining serial block-face images from frozen blocks or paraffin-embedded tissue, and reconstructed 3D images from various specimens, such as mouse and zebrafish embryos 3 , rat knee joint 4 , whole adult mouse 5, 6 , and equine ovary 7 . However, these types of apparatus are not widely used.…”

Section: Introductionmentioning

confidence: 99%

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Tajika

Murakami

Iijima

et al. 2017

Sci Rep

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We have developed an imaging method designated as correlative light microscopy and block-face imaging (CoMBI), which contributes to improve the reliability of morphological analyses. This method can collect both the frozen sections and serial block-face images in a single specimen. The frozen section can be used for conventional light microscopic analysis to obtain 2-dimensional (2D) anatomical and molecular information, while serial block-face images can be used as 3-dimensional (3D) volume data for anatomical analysis. Thus, the sections maintain positional information in the specimen, and allows the correlation of 2D microscopic data and 3D volume data in a single specimen. The subjects can vary in size and type, and can cover most specimens encountered in biology. In addition, the required system for our method is characterized by cost-effectiveness. Here, we demonstrated the utility of CoMBI using specimens ranging in size from several millimeters to several centimeters, i.e., mouse embryos, human brainstem samples, and stag beetle larvae, and present successful correlation between the 2D light microscopic images and 3D volume data in a single specimen.

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Section: Introductionmentioning

confidence: 99%

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Tajika

Murakami

Iijima

et al. 2017

Sci Rep

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“…Block-face imaging systems for frozen blocks have been developed and released from several research groups. For frozen specimens, Yokota’s group at RIKEN developed a 3D internal structure microscope for frozen biological specimens 26 , and they improved the instruments to widen the imaging target, such as metal materials 27 . Wilson and Roy developed a cryo-imaging device for imaging frozen biological specimens 4 , and they put it on the market (BioInVision, USA).…”

Section: Discussionmentioning

confidence: 99%

Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens

Ishii

Tajika

Murakami

et al. 2021

Sci Rep

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Correlative microscopy and block-face imaging (CoMBI), a method that we previously developed, is characterized by the ability to correlate between serial block-face images as 3-dimensional (3D) datasets and sections as 2-dimensional (2D) microscopic images. CoMBI has been performed for the morphological analyses of various biological specimens, and its use is expanding. However, the conventional CoMBI system utilizes a cryostat, which limits its compatibility to only frozen blocks and the resolution of the block-face image. We developed a new CoMBI system that can be applied to not only frozen blocks but also paraffin blocks, and it has an improved magnification for block-face imaging. The new system, called CoMBI-S, comprises sliding-type sectioning devices and imaging devices, and it conducts block slicing and block-face imaging automatically. Sections can also be collected and processed for microscopy as required. We also developed sample preparation methods for improving the qualities of the block-face images and 3D rendered volumes. We successfully obtained correlative 3D datasets and 2D microscopic images of zebrafish, mice, and fruit flies, which were paraffin-embedded or frozen. In addition, the 3D datasets at the highest magnification could depict a single neuron and bile canaliculus.

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“…[ 15 ] reported detailed analysis of the internal anatomical structure of the equine ovary using three-dimensional internal structure microscopy (3D-ISM), which consists of a computer-controlled slicer and a CCD camera. In addition, three-dimensional reconstructed images of the equine ovary from more than 1,000 sliced images can provide quantifiable information of follicles and CL and reveal the population of the follicles as small as 1 mm 3 in volume [ 12 , 16 , 17 ]. The morphology of prepubertal mare ovary has been studied through several techniques; macroscopic morphology [ 8 , 18 , 26 ], histology [ 8 , 24 ] and ultrasonography [ 18 , 20 ].…”

mentioning

confidence: 99%

Analysis of the equine ovarian structure during the first twelve months of life by three-dimensional internal structure microscopy

Ono

Akuzawa²,

Nambo

et al. 2015

The Journal of Veterinary Medical Science

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A three-dimensional internal structure microscopy (3D-ISM) can clarify the anatomical arrangement of internal structures of equine ovaries. In this study, morphological changes of the equine ovary over the first 12 months of life were investigated by 3D-ISM in 59 fillies and by histological analysis in 2 fillies. The weight and volume of the paired ovaries initially decreased from 0 to 1 months to 2 to 3 months of age and then significantly increased at 8 to 12 months of age. The ovulation fossa was first observed around the 3rd month and became evident after the 6th month. The number of follicles with a diameter of ≥10 mm and the diameter of the largest follicle increased gradually after 6 months of age. On a volume basis, the medulla accounted for nearly 90% of the whole ovary at 0 to 1 months of age, but significantly decreased from 2 to 3 months of age. The volume of the cortex increased progressively after birth and reached approximately 60% of the total volume at 8 to 12 months of age. This significant development of the cortex coincided with the increased number and size of large follicles observed from 6 months of age. These results suggest that the development of the cortex plays a role in the maturation of the follicles and the equine ovary undergoes substantial morphological changes postnatally until puberty.

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Three‐dimensional Reconstruction of the Equine Ovary

Cited by 18 publications

References 11 publications

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

A novel imaging method for correlating 2D light microscopic data and 3D volume data based on block-face imaging

Correlative microscopy and block-face imaging (CoMBI) method for both paraffin-embedded and frozen specimens

Analysis of the equine ovarian structure during the first twelve months of life by three-dimensional internal structure microscopy

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