Three-dimensional reconstruction of the antennal lobe inDrosophila melanogaster

Laissue, Pierre Philippe; Reiter, Christian; Hiesinger, P. Robin; Halter, Susan A.; Fischbach, Karl‐Friedrich; Stocker, Reinhard F.

doi:10.1002/(sici)1096-9861(19990322)405:4<543::aid-cne7>3.0.co;2-a

Cited by 390 publications

(91 citation statements)

References 40 publications

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“…To address this possibility, we imaged and reconstructed two additional D. melanogaster antennal lobes, one from an orco −/− ant and one from a wild-type individual. These reconstructions were not performed strictly de novo , as the O. biroi and D. melanogaster reconstructions reported in the main text, but by referring to the published map of the D. melanogaster antennal lobe (Laissue and Vosshall, 2008; Laissue et al, 1999). Due to differences in sample preparation and imaging methods, it was not possible to unambiguously match each glomerulus to the published map.…”

Section: Star Methodsmentioning

confidence: 99%

orco Mutagenesis Causes Loss of Antennal Lobe Glomeruli and Impaired Social Behavior in Ants

Trible

Olivos-Cisneros

McKenzie

et al. 2017

Cell

235

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Summary Life inside ant colonies is orchestrated with diverse pheromones, but it is not clear how ants perceive these social signals. It has been proposed that pheromone perception in ants evolved via expansions in the numbers of odorant receptors (ORs) and antennal lobe glomeruli. Here we generate the first mutant lines in the clonal raider ant, Ooceraea biroi, by disrupting orco, a gene required for the function of all ORs. We find that orco mutants exhibit severe deficiencies in social behavior and fitness, suggesting they are unable to perceive pheromones. Surprisingly, unlike in Drosophila melanogaster, orco mutant ants also lack most of the approximately 500 antennal lobe glomeruli found in wild-type ants. These results illustrate that ORs are essential for ant social organization and raise the possibility that, similar to mammals, receptor function is required for the development and/or maintenance of the highly complex olfactory processing areas in the ant brain.

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Section: Star Methodsmentioning

confidence: 99%

orco Mutagenesis Causes Loss of Antennal Lobe Glomeruli and Impaired Social Behavior in Ants

Trible

Olivos-Cisneros

McKenzie

et al. 2017

Cell

235

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“…Third instar larvae were dissected as previously described (Mosca and Schwarz, 2010). The following primary antibodies were used: mouse antibody to Bruchpilot (1:40, DSHB, Catalog #mAbnc82, Iowa City, IA) (Laissue et al, 1999), rabbit antibody to Synaptotagmin I (1:4000) (Mackler et al, 2002), rat antibody to N-Cadherin (1:40, DSHB, Catalog #mAbDN-EX #8, Iowa City, IA) (Iwai et al, 1997), rat antibody to HA (1:100, Roche, Catalog #11867423001, Basel, Switzerland), mouse antibody to choline acetyltransferase (ChAT) (1:100, DSHB, Catalog #mAbChAT4B1, Iowa City, IA) (Takagawa and Salvaterra, 1996), mouse antibody to ELAV (DSHB, mAb9F8A9, 1:100) (O'Neill et al, 1994), rabbit antibody to GABA (1:200, Sigma-Aldrich, Catalog #A2052, St. Louis, MO), mouse antibody to Repo (1:100, DSHB, Catalog #mAb8D12, Iowa City, IA) (Alfonso and Jones, 2002), rabbit antibody to vGlut (1:500) (Daniels et al, 2008), rabbit antibody to dsRed (1:250, Clontech, Catalog #632496, Mountain View, CA), chicken antibody to GFP (1:1000, Aves Labs, Catalog #GFP-1020, Tigard, OR), Alexa647-conjugated goat antibody to HRP (1:100, Jackson ImmunoResearch, Catalog #123-605-021, West Grove, PA). Alexa488-, Alexa568-, and Alexa647-conjugated secondary antibodies were used at 1:250 (ThermoFisher Scientific and Jackson ImmunoResearch, Various Catalog #s).…”

Section: Methodsmentioning

confidence: 99%

Presynaptic LRP4 promotes synapse number and function of excitatory CNS neurons

Mosca

Luginbuhl

Wang

et al. 2017

eLife

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Precise coordination of synaptic connections ensures proper information flow within circuits. The activity of presynaptic organizing molecules signaling to downstream pathways is essential for such coordination, though such entities remain incompletely known. We show that LRP4, a conserved transmembrane protein known for its postsynaptic roles, functions presynaptically as an organizing molecule. In the Drosophila brain, LRP4 localizes to the nerve terminals at or near active zones. Loss of presynaptic LRP4 reduces excitatory (not inhibitory) synapse number, impairs active zone architecture, and abolishes olfactory attraction - the latter of which can be suppressed by reducing presynaptic GABAB receptors. LRP4 overexpression increases synapse number in excitatory and inhibitory neurons, suggesting an instructive role and a common downstream synapse addition pathway. Mechanistically, LRP4 functions via the conserved kinase SRPK79D to ensure normal synapse number and behavior. This highlights a presynaptic function for LRP4, enabling deeper understanding of how synapse organization is coordinated.DOI: http://dx.doi.org/10.7554/eLife.27347.001

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“…Immunohistochemistry was performed as described previously 25 . Primary antibodies were obtained from the following sources (with dilutions in parentheses): mouse nc82 from the Developmental Studies Hybridoma Bank 35 (nc82-s, 1:50), rat anti-CD8 from Invitrogen (MCD0800, 1:200), rabbit anti-GABA from Sigma 25 (A2052, 1:200), rabbit anti-dVGluT (1:500; gift of Aaron DiAntonio, Washington University, St. Louis, ref. 19 ).…”

Section: Methodsmentioning

confidence: 99%

Thermosensory processing in the Drosophila brain

Liu

Mazor

Wilson

2015

Nature

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In Drosophila, just as in vertebrates, changes in external temperature are encoded by bidirectional opponent thermoreceptor cells: some cells are excited by warming and inhibited by cooling, whereas others are excited by cooling and inhibited by warming1,2. The central circuits that process these signals are not understood. In Drosophila, a specific brain region receives input from thermoreceptor cells2,3. Here we show that distinct genetically-identified projection neurons (PNs) in this brain region are excited by cooling, warming, or both. The PNs excited by cooling receive mainly feedforward excitation from cool thermoreceptors. In contrast, the PNs excited by warming (“warm-PNs”) receive both excitation from warm thermoreceptors and crossover inhibition from cool thermoreceptors via inhibitory interneurons. Notably, this crossover inhibition elicits warming-evoked excitation, because warming suppresses tonic activity in cool thermoreceptors. This in turn disinhibits warm-PNs and sums with feedforward excitation evoked by warming. Crossover inhibition could cancel non-thermal activity (noise) that is positively-correlated among warm and cool thermoreceptor cells, while reinforcing thermal activity which is anti-correlated. Our results show how central circuits can combine signals from bidirectional opponent neurons to construct sensitive and robust neural codes.

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Three-dimensional reconstruction of the antennal lobe inDrosophila melanogaster

Cited by 390 publications

References 40 publications

orco Mutagenesis Causes Loss of Antennal Lobe Glomeruli and Impaired Social Behavior in Ants

orco Mutagenesis Causes Loss of Antennal Lobe Glomeruli and Impaired Social Behavior in Ants

Presynaptic LRP4 promotes synapse number and function of excitatory CNS neurons

Thermosensory processing in the Drosophila brain

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