Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects

Zhao, Shichang; Zhang, Jianhua; Zhu, Min; Zhang, Yadong; Liu, Zhongtang; Tao, Cuilian; Zhu, Yufang; Zhang, Changqing

doi:10.1016/j.actbio.2014.10.015

Cited by 143 publications

(123 citation statements)

References 51 publications

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“…Thus, this study supports previously published pre-clinical in vivo reports, where various Sr-loaded bone substitute materials were shown to enhance bone formation in a variety of models, including the calvarial CSD in rats as herein [19,21,24,26]. For instance, 5 mm of CSD grafted with Sr-loaded bioglass particles showed significantly larger amounts of new bone compared with defects grafted with non-loaded bioglass or with non-grafted controls, after 8 weeks of healing [21].…”

Section: Discussionsupporting

confidence: 79%

“…Previous reports have shown that Sr-loaded biomaterial scaffolds exhibited increase resorption/dissolution comparing with uncoated ones [21,22]. For instance, a calcium-silicon ceramic [22] and a mesoporous bioactive glass scaffold [21] were more readily resorbed when loaded with Sr.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, 5 mm of CSD grafted with Sr-loaded bioglass particles showed significantly larger amounts of new bone compared with defects grafted with non-loaded bioglass or with non-grafted controls, after 8 weeks of healing [21]. In another study, using the same model, defects grafted with a collagen-hydroxyapatite-Sr construct showed increased radiographic bone density and bone formation compared to defects grafted with the collagen-hydroxyapatite construct without Sr or non-grafted controls at 12 weeks [26].…”

Section: Discussionmentioning

confidence: 99%

“…Systemic administration of Sr (in the form of Sr ranelate-SrR) has been shown to improve bone quality and reduces the risk of fractures both in females with postmenopausal osteoporosis and osteoporotic men [17,18]. More recently, several experimental studies have shown promising results in terms of increased bone formation after Sr enrichment of various bone substitute materials, such as allogeneic bone [19], bioactive bioglass [20,21], calciumsilicon ceramic [22], calcium phosphate [23,24], and hydroxyapatite [25,26]. Furthermore, it was observed in recent experiments that Sr-loaded titanium implants presented significantly increased bone-to-implant contact, comparing to control implants [27][28][29].…”

Section: Introductionmentioning

confidence: 98%

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Loading deproteinized bovine bone with strontium enhances bone regeneration in rat calvarial critical size defects

et al. 2018

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Objective To evaluate the effect of grafting with strontium (Sr)-loaded deproteinized bovine bone (DBB) on bone healing in calvarial critical size defects (CSD) in rats. Material and methods Two circular bone defects (5 mm in diameter) were created in the calvaria of 42 rats. One of the defects, randomly chosen, was grafted with (a) DBB, (b) DBB loaded with 19.6 μg/g of Sr (DBB/Sr1), or (c) DBB loaded with 98.1 μg/g of Sr (DBB/Sr2). The other defect was left empty as negative control. Groups of seven animals from each of the groups were euthanized 15 and 60 days post-op. Bone healing in the CSD was evaluated by micro-CT and histology/histomorphometry and immunohistochemistry.Results DBB/Sr2-grafted sites showed statistically significantly shorter radiographic residual defect length compared with DBB/ Sr1-and DBB-grafted sites, and with empty controls at 60 days. Further, the amount of new bone formation in the DBB/Sr1-and DBB/Sr2-grafted sites was significantly higher compared with that in the DBB-grafted sites at 60 days. A larger number of DBB/ Sr1-and DBB/Sr2-grafted sites presented with no-or only limited to mild inflammation, compared with the DBB-grafted sites, especially at 60 days. Higher expression of osteocalcin was observed in DBB/Sr1-and DBB/Sr2-grafted sites as compared to DBB-grafted sites. Conclusion Grafting with Sr-loaded DBB enhanced bone formation in CSD in rats, when compared with grafting with nonloaded DBB. Clinical relevance Grafting with Sr-loaded DBB may enhance bone formation in bone defects.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Loading deproteinized bovine bone with strontium enhances bone regeneration in rat calvarial critical size defects

et al. 2018

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“…Generally, a ceramic consists of crystal phase, amorphous phase, and pores, and the higher crystallinity at higher sintering temperature induces more density and less pores, which might contribute the enhanced compressive strength for β-Ca 2 SiO 4 scaffolds sintered at 1200 °C compared to those scaffolds sintered at 1000 °C and 1100 °C. The results indicated that the β-Ca 2 SiO 4 scaffolds meet the requirement of the compressive strength of cancellous bone (2–12 MPa) [48,50]. …”

Section: Discussionmentioning

confidence: 99%

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties

Liu

et al. 2018

Science and Technology of Advanced Materials

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Silicate bioceramic scaffolds are of great interest in bone tissue engineering, but the fabrication of silicate bioceramic scaffolds with complex geometries is still challenging. In this study, three-dimensional (3D) porous β-Ca2SiO4 scaffolds have been successfully fabricated from preceramic resin loaded with CaCO3 active filler by 3D printing. The fabricated β-Ca2SiO4 scaffolds had uniform interconnected macropores (ca. 400 μm), high porosity (>78%), enhanced mechanical strength (ca. 5.2 MPa), and excellent apatite mineralization ability. Importantly, the results showed that the increase of sintering temperature significantly enhanced the compressive strength and the scaffolds sintered at higher sintering temperature stimulated the adhesion, proliferation, alkaline phosphatase activity, and osteogenic-related gene expression of rat bone mesenchymal stem cells. Therefore, the 3D printed β-Ca2SiO4 scaffolds derived from preceramic resin and CaCO3 active fillers would be promising candidates for bone tissue engineering.

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3D‐Plotted Beta‐Tricalcium Phosphate Scaffolds with Smaller Pore Sizes Improve In Vivo Bone Regeneration and Biomechanical Properties in a Critical‐Sized Calvarial Defect Rat Model

Diao

Ouyang

Deng

et al. 2018

Adv Healthcare Materials

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Due to the difficulty in fabricating bioceramic scaffolds with smaller pore sizes by the current 3D printing technique, the effect of smaller pore sizes (below 400 µm) of 3D printed bioceramic scaffolds on the bone regeneration and biomechanical behavior is never studied. Herein beta-tricalcium phosphate (β-TCP) scaffolds with interconnected smaller pores of three different sizes (100, 250, and 400 µm) are fabricated by 3D plotting. The resultant scaffolds are then implanted into rat critical-sized calvarial defects without any seeded cells. A custom-designed device is developed to investigate the biomechanical properties of the scaffolds after surgical implantation for 4, 8, and 12 weeks. The scaffolds with the 100 µm pore size are found to present the highest maximum load and stiffness, comparable to those of the autogenous bone, after being implanted for 12 weeks. Micro-computed tomography (micro-CT) and histological analysis further indicate that the scaffolds with the 100 µm pore size achieve the highest percentage of new bone ingrowth, which correlates to their best in vivo biomechanical properties. This study demonstrates that tailoring the pore size of β-TCP scaffolds to a smaller range by 3D-plotting can be a facile and efficient approach to enhanced bone regeneration and biomechanical behaviors in bone repair.

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Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects

Cited by 143 publications

References 51 publications

Loading deproteinized bovine bone with strontium enhances bone regeneration in rat calvarial critical size defects

Loading deproteinized bovine bone with strontium enhances bone regeneration in rat calvarial critical size defects

3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties

3D‐Plotted Beta‐Tricalcium Phosphate Scaffolds with Smaller Pore Sizes Improve In Vivo Bone Regeneration and Biomechanical Properties in a Critical‐Sized Calvarial Defect Rat Model

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Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects

Cited by 143 publications

References 51 publications

Loading deproteinized bovine bone with strontium enhances bone regeneration in rat calvarial critical size defects

Loading deproteinized bovine bone with strontium enhances bone regeneration in rat calvarial critical size defects

3D printed porous β-Ca2SiO4scaffolds derived from preceramic resin and their physicochemical and biological properties

3D‐Plotted Beta‐Tricalcium Phosphate Scaffolds with Smaller Pore Sizes Improve In Vivo Bone Regeneration and Biomechanical Properties in a Critical‐Sized Calvarial Defect Rat Model

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3D printed porous β-Ca₂SiO₄scaffolds derived from preceramic resin and their physicochemical and biological properties