Three-Dimensional Printable Gelatin Hydrogels Incorporating Graphene Oxide to Enable Spontaneous Myogenic Differentiation

Kang, Moon Sung; Kang, Jeon Il; Thi, Phuong Le; Park, Kyung Min; Hong, Suck Won; Choi, Yu Suk; Han, Dong‐Wook; Парк, Ки Донг

doi:10.1021/acsmacrolett.0c00845

Cited by 40 publications

(34 citation statements)

References 48 publications

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“…It was hypothesized that the cell density (5 x 10 6 cells/ml) was not enough to promote cell-cell communication and promote the alignment existing the skeletal muscle, as also reported by others. 41 This was in line with what was observed elsewhere 15,42 and can be explained by the enhanced cell-cell contact that promotes an enhanced inter-cellular communication, as previously reported by others. 43 Thus, the potential of this construct to maturate into a functional contractile muscle surrogate will be further evaluated in the future.…”

Section: Discussionsupporting

confidence: 90%

3D bioprinting of gellan gum‐based hydrogels tethered with laminin‐derived peptides for improved cellular behavior

Alheib

Silva

Youn

et al. 2022

J Biomedical Materials Res

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The treatment of skeletal muscle defects is still a topic of noteworthy concern since surgical intervention is not capable of recovering muscle function. Herein, we propose myoblasts laden in laminin-inspired biofunctionalized gellan gum hydrogels as promising tissue-engineered skeletal muscle surrogates. Gellan gum-based hydrogels were developed by combining native gellan gum (GG) and GG tethered with lamininderived peptides (CIKVAVS (V), KNRLTIELEVRTC (T) or RKRLQVQLSIRTC (Q)), using different polymer content (0.75%-1.875%). Hydrogels were characterized in terms of compressive modulus, molecules trafficking, and C2C12 adhesion. Hydrogels with higher polymeric content (1.125%-1.875%) showed higher stiffness whereas hydrogels with lower polymer content (0.75%-1.125%) showed higher fluorescein isothiocyanate-dextran molecules diffusion. Cell spreading was achieved regardless of the laminin-derived peptide but preferred in hydrogels with higher polymer content (1.125%-1.875%). Taken together, hydrogels with 1.125% of polymer content were selected for printability analysis. GG-based inks showed a non-newtonian, shear-thinning, and thixotropic behavior suitable for printing. Accordingly, all inks were printable, but inks tethered with T and Q peptides presented some signs of clogging. Cell viability was affected after printing but increased after 7 days of culture. After 7 days, cells were spreading but not showing significant signs of cell-cell communications. Therefore, cell density was increased, thus, myocytes loaded in V-tethered GG-based inks showed higher cell-cell communication, spreading morphology, and alignment 7, 14 days post-printing. Overall, myoblasts laden in laminin-inspired biofunctionalized GG-based hydrogels are a promising skeletal muscle surrogate with the potential to be used as in vitro model or explored for further in vivo applications.

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Section: Discussionsupporting

confidence: 90%

3D bioprinting of gellan gum‐based hydrogels tethered with laminin‐derived peptides for improved cellular behavior

Alheib

Silva

Youn

et al. 2022

J Biomedical Materials Res

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“…Kang et al [111] GO/GelMA/PCL Nanofibers Peripheral nerve regeneration rGO improved the mechanical and electrical properties of the formulation and, at lower concentration of about 0.25-0.5 wt%, enhanced Schwann cell (RSC96) proliferation.…”

Section: Ryu Et Al [109]mentioning

confidence: 99%

Graphene Oxide–Protein-Based Scaffolds for Tissue Engineering: Recent Advances and Applications

et al. 2022

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The field of tissue engineering is constantly evolving as it aims to develop bioengineered and functional tissues and organs for repair or replacement. Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. This review critically focuses on opportunities to employ protein–graphene oxide structures either as nanocomposites or as biocomplexes and highlights the effects of carbonaceous nanostructures on protein conformation and structural stability for applications in tissue engineering and regenerative medicine. Herein, recent applications and the biological activity of nanocomposite bioconjugates are analyzed with respect to cell viability and proliferation, along with the ability of these constructs to sustain the formation of new and functional tissue. Novel strategies and approaches based on stem cell therapy, as well as the involvement of the extracellular matrix in the design of smart nanoplatforms, are discussed.

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“…The natural ECM exhibits biochemical and topographical signals, which influence cell behavior like cell viability, morphology, proliferation, and differentiation 9 , 67 . The physico-chemical properties of various scaffold materials can also be used to aid muscle tissue formation.…”

Section: Fabrication Strategiesmentioning

confidence: 99%

Recent trends in bioartificial muscle engineering and their applications in cultured meat, biorobotic systems and biohybrid implants

Schätzlein

Blaeser

2022

Commun Biol

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Recent advances in tissue engineering and biofabrication technology have yielded a plethora of biological tissues. Among these, engineering of bioartificial muscle stands out for its exceptional versatility and its wide range of applications. From the food industry to the technology sector and medicine, the development of this tissue has the potential to affect many different industries at once. However, to date, the biofabrication of cultured meat, biorobotic systems, and bioartificial muscle implants are still considered in isolation by individual peer groups. To establish common ground and share advances, this review outlines application-specific requirements for muscle tissue generation and provides a comprehensive overview of commonly used biofabrication strategies and current application trends. By solving the individual challenges and merging various expertise, synergetic leaps of innovation that inspire each other can be expected in all three industries in the future.

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Three-Dimensional Printable Gelatin Hydrogels Incorporating Graphene Oxide to Enable Spontaneous Myogenic Differentiation

Cited by 40 publications

References 48 publications

3D bioprinting of gellan gum‐based hydrogels tethered with laminin‐derived peptides for improved cellular behavior

3D bioprinting of gellan gum‐based hydrogels tethered with laminin‐derived peptides for improved cellular behavior

Graphene Oxide–Protein-Based Scaffolds for Tissue Engineering: Recent Advances and Applications

Recent trends in bioartificial muscle engineering and their applications in cultured meat, biorobotic systems and biohybrid implants

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