Three-dimensional polymer scaffolds for high throughput cell-based assay systems

Performance Evaluation of 3D Polystyrene 96-Well Plates with Human Neural Stem Cells in a Calcium Assay

“…Human neural stem cells or neural progenitors (NPs) were maintained as described in Cheng et al 2 The growth medium for NP cells was neural basal medium (Invitrogen) supplemented with penicillin/streptomycin, L-glutamine, recombinant hLIF, bFGF, and B-27. The medium was half changed every 48 h. For subculturing, 90% confluent cells were aspirated by pipetting, and the subculture ratio was typically 1:2 to 1:3.…”

“…The fabrication process of the porous polystyrene 3D 96-well plates was modified from a process previously published by Cheng et al 2 The Biomek NP liquid handler was used to implement automation into the fabrication process. Before use, the Biomek NP was calibrated according to the manufacturer's instructions to ensure the best accuracy and precision.…”

“…After chloroform was completely evaporated, the plates were collected and immersed into 37 °C water to ensure complete removal of the salt particles. The baking process described in Cheng et al 2 was omitted for the 96-well plates because the adhesive used to glue the glass to the plates was sensitive to heat. The plates were sterilized by UV light in a biohazard laminar flow hood overnight.…”

Development and molecular characterization of polymeric micro-nanofibrous scaffold of a defined 3-D niche for in vitro chemosensitivity analysis against acute myeloid leukemia cells

“…[5][6][7][8][9][10][11][12] Although there have been attempts toward the fabrication of scaffolds that resemble the bone marrow niche with better cell adhesion and proliferation compared to 2-D matrices, the development of a micro-nanofibrous environment close to the bone marrow niche, without any added stromal cells, showing similar in vivo molecular characteristics to CAMDR has not yet been shown, as far as we are aware. 4,12,17,35 Thus, the fabrication of a micro-nanofibrous structure close to the bone marrow niche, as shown in Figure 1V, was envisaged using suitable composites of polymers and TIPS technology for scaffold preparation.…”

“…Thus, 3-D cell culture systems could provide a possible solution for this problem if one could simulate the bone marrow niche to a greater extent. 4,16,17 There have been attempts to generate 3-D environments using the synthetic polymer polyurethane (PU) for the culture of AML cell lines. 12 Similarly, mouse hematopoietic stem cells (HSCs) have been cultured on 3-D polymeric PU scaffoldbased ex vivo systems.…”