Three-dimensional piezoelectric fibrous scaffolds selectively promote mesenchymal stem cell differentiation

Damaraju, Sita M.; Shen, Yueyang; Elele, Ezinwa; Khusid, Boris; Eshghinejad, Ahmad; Li, Jiangyu; Jaffé, Michael; Arinzeh, Treena Livingston

doi:10.1016/j.biomaterials.2017.09.024

Cited by 191 publications

(173 citation statements)

References 59 publications

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“…Similarly, it has been recently demonstrated that fibrous PVDF‐TrFE scaffolds could be used for stimulating chondrogenic and osteogenic differentiation of human mesenchymal stem cells, and corresponding extracellular matrix/tissue formation in physiological loading conditions. The effect was attributed to the voltage output exhibited by the scaffolds …”

Section: Resultsmentioning

confidence: 99%

Electrospun Fibrous PVDF‐TrFe Scaffolds for Cardiac Tissue Engineering, Differentiation, and Maturation

Adadi

Yadid

Gal

et al. 2020

Adv Materials Technologies

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Cardiac tissue engineering aims to create cardiac tissue constructs that recapitulate the structure and function of the native heart. This approach has been widely used for creating myocardial implants for regenerative medicine, and more recently, for developing in vitro cardiotoxicity screening assays. However, once the engineered myocardial tissues are implanted or subjected to pharmacological stimuli, their performance should be monitored. Currently, there is no biomaterial that promotes functional tissues assembly while providing real‐time information about their function, in situ. In this study, the piezoelectric phenomenon is sought to be exploited, to measure the contractions generated by engineered cardiac tissues. A poly‐(vinylidene fluoride) (PVDF)‐based electrospun fiber scaffold is developed, and it is hypothesized that the contractions of cardiomyocytes in the scaffold will induce mechanical deformations, which will result in measurable electric voltage. The PVDF scaffolds are characterized and optimized for supporting formation of aligned, functional, cardiac tissues. The scaffolds' function is then validated as sensors for tissue contraction and it is demonstrated that they can sense contractions of tissues constructed from as few as 5 × 105 cardiomyocytes. Furthermore, it is demonstrated that human induced pluripotent stem cells can be directly seeded and differentiated to cardiomyocytes, and then mature over the course of 40 days on the PVDF fiber scaffolds.

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Section: Resultsmentioning

confidence: 99%

Electrospun Fibrous PVDF‐TrFe Scaffolds for Cardiac Tissue Engineering, Differentiation, and Maturation

Adadi

Yadid

Gal

et al. 2020

Adv Materials Technologies

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“…While the introduction of voids into polymers leads to formation of cellular and porous polymer electrets which may exhibit much higher d 33 values (>400 pC N −1 ) as summarized in recent reviews, we focus on the discussion on intrinsic bulk piezoelectric response in this progress report. Recent studies show that these polymers are ideal for flexible and biocompatible applications in energy harvesters, sensors, actuators, and so on . To foster these promising applications, it demands a large improvement of the modest piezoelectric coefficients of the polymers, which directly determines the efficiency of piezoelectric energy harvesting and the performance of sensors and actuators .…”

Section: Introductionmentioning

confidence: 99%

Ferroelectric Polymers Exhibiting Negative Longitudinal Piezoelectric Coefficient: Progress and Prospects

Liu

Wang

2020

Advanced Science

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Piezoelectric polymers are well‐recognized to hold great promise for a wide range of flexible, wearable, and biocompatible applications. Among the known piezoelectric polymers, ferroelectric polymers represented by poly(vinylidene fluoride) and its copolymer poly(vinylidene fluoride‐co‐trifluoroethylene) possess the best piezoelectric coefficients. However, the physical origin of negative longitudinal piezoelectric coefficients occurring in the polymers remains elusive. To address this long‐standing challenge, several theoretical models proposed over the past decades, which are controversial in nature, have been revisited and reviewed. It is concluded that negative longitudinal piezoelectric coefficients arise from the negative longitudinal electrostriction in the crystalline domain of the polymers, independent of amorphous and crystalline‐amorphous interfacial regions. The crystalline origin of piezoelectricity offers unprecedented opportunities to improve electromechanical properties of polymers via structural engineering, i.e., design of morphotropic phase boundaries in ferroelectric polymers.

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“…A distinctive feature of the PVDF-TrFE scaffolds is that they are piezoelectric 37 , similar to cellulose found in the cell wall 38 . To examine the piezoelectric property's effect on cell attachment, corona poling was used to enhance the piezoelectric properties of PDVF-TrFE scaffolds ( Figure 4A).…”

Section: The Piezoelectric Property Of Pvdf-trfe Scaffolds Contributementioning

confidence: 99%

“…Tensile testing was performed to determine the Young's modulus and maximum tensile stress of PVDF-TrFE matrices. Tensile tests were performed using a mechanical tester (Instron, MA, USA), as previously described 37 . Briefly, matrices were cut into 70 mm x 10 mm strips.…”

Section: Mechanical Testingmentioning

confidence: 99%

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Artificial scaffolds that mimic the plant extracellular environment for the culture and attachment of plant cells

Calcutt

Vincent

Dean

et al. 2020

Preprint

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Plant growth and development involves an intricate program of cell division and cell expansionto generate different cell types, tissue patterns and organ shapes. Plant cells are stuck together by their cell walls and the spatial context of cells within tissues plays a critical role in cell fate specification and morphogenesis. An in vitro model system to study plant development and its regulation by various extrinsic and intrinsic factors requires the ability to mimic the physical interactions between cells and their environment. Here, we present a set of artificial scaffolds to which cultured tobacco BY-2 cells adhere without causing morphological abnormalities. These scaffolds mimic native plant cell walls in terms of their fibrous nature, charge, hydrophobicity and piezoelectricity. We found that the extent of plant cell adhesion was essentially insensitive to the stiffness, fiber dimension, and fiber orientation of the scaffolds, but was affected by the piezoelectric properties of scaffolds where adhesion increased on piezoelectric materials. We also found that the plant cell wall polysaccharide, pectin, is largely responsible for adhesion to scaffolds, analogous to pectin-mediated adhesion of plant cells in tissues. Together, this work establishes biomimetic scaffolds that realistically emulate the plant tissue environment and provide the capability to develop microfluidic devices to study how cell-cell and cell-matrix interactions affect plant developmental pathways.

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Three-dimensional piezoelectric fibrous scaffolds selectively promote mesenchymal stem cell differentiation

Cited by 191 publications

References 59 publications

Electrospun Fibrous PVDF‐TrFe Scaffolds for Cardiac Tissue Engineering, Differentiation, and Maturation

Electrospun Fibrous PVDF‐TrFe Scaffolds for Cardiac Tissue Engineering, Differentiation, and Maturation

Ferroelectric Polymers Exhibiting Negative Longitudinal Piezoelectric Coefficient: Progress and Prospects

Artificial scaffolds that mimic the plant extracellular environment for the culture and attachment of plant cells

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