Three-dimensional optical force field on a Chinese hamster ovary cell in a fiber-optical dual-beam trap

Wei, Ming-Tzo; Yang, Kun-Ta; Karmenyan, Artashes; Chiou, Arthur

doi:10.1364/oe.14.003056

Cited by 42 publications

(27 citation statements)

References 12 publications

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“…The knowledge of the beam properties enables the detailed calculation of the trapping force fields within the capillary (see Fig. 3), which compare well with other recent theoretical calculations and experimental measurements (Knöner et al 2006;Wei et al 2006). This, in turn, provides the basis for an informed positioning of the capillary relative to the laser beams.…”

Section: Discussionsupporting

confidence: 80%

“…Additionally, for nondeformation purposes, the dual-beam laser trap is gaining importance relative to the all-pervasive optical tweezers as evidenced by recent theoretical and experimental studies (Gauthier et al 2003;Wei et al 2006;Jess et al 2006;Cran-McGreehin et al 2006). The dual-beam trap nature and its independence from any high-NA focusing renders it ideally suited for microfluidic integration.…”

Section: Discussionmentioning

confidence: 99%

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Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications

Lincoln

Schinkinger

Travis

et al. 2007

Biomed Microdevices

124

106

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A dual-beam fiber laser trap, termed the optical stretcher when used to deform objects, has been combined with a capillary-based microfluidic system in order to serially trap and deform biological cells. The design allows for control over the size and position of the trap relative to the flow channel. Data is recorded using video phase contrast microscopy and is subsequently analyzed using a custom edge fitting routine. This setup has been regularly used with measuring rates of 50-100 cells/h. One such experiment is presented to compare the distribution of deformability found within a normal epithelial cell line to that of a cancerous one. In general, this microfluidic optical stretcher can be used for the characterization of cells by their viscoelastic signature. Possible applications include the cytological diagnosis of cancer and the gentle and marker-free sorting of stem cells from heterogeneous populations for therapeutic cell-based approaches in regenerative medicine.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications

Lincoln

Schinkinger

Travis

et al. 2007

Biomed Microdevices

124

106

View full text Add to dashboard Cite

show abstract

“…Dielectrophoresis calculations show that pN forces are possible in our channel for reasonably chosen voltages. Using fiber-optic dual-beam trapping, Wei et al 29 determined the force constants of Chinese hamster ovary cells to be on the order of pN/ m. Wanichapichart et al 30 determined the elastic constant of Dendrobium protoplasts by elongating them in ac fields. The elastic constant of their membrane varied nonlinearly with field strength ranging from 40 to 80 pN/ m. 30 Those studies suggest that deformation experiments on these model systems should be feasible with the new system.…”

Section: Discussionmentioning

confidence: 99%

Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells

et al. 2008

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The mechanical behavior of cells offers insight into many aspects of their properties. We propose an approach to the mechanical analysis of cells that uses a combination of electromanipulation for stimulus and capacitance for sensing. To demonstrate this approach, polystyrene spheres and yeast cells flowing in a 25 m ϫ 100 m microfluidic channel were detected by a perpendicular pair of gold thin film electrodes in the channel, spaced 25 m apart. The presence of cells was detected by capacitance changes between the gold electrodes. The capacitance sensor was a resonant coaxial radio frequency cavity ͑2.3 GHz͒ coupled to the electrodes. The presence of yeast cells ͑Saccharomyces cerevisiae͒ and polystyrene spheres resulted in capacitance changes of approximately 10 and 100 attoFarad ͑aF͒, respectively, with an achieved capacitance resolution of less than 2 aF in a 30 Hz bandwidth. The resolution is better than previously reported in the literature, and the capacitance changes are in agreement with values estimated by finite element simulations. Yeast cells were trapped using dielectrophoretic forces by applying a 3 V signal at 1 MHz between the electrodes. After trapping, the cells were displaced using amplitude and frequency modulated voltages to produce modulated dielectrophoretic forces. Repetitive displacement and relaxation of these cells was observed using both capacitance and video microscopy.

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“…58 Using this method, the stiffness is measured using the complete distribution of the trapped particle's positions within the optical trap. 59,60 Alternatively, using the power spectral density (PSD) method, a trapped particle's thermal noise is analyzed in the frequency domain to determine the optical trap's stiffness. 41 The third (direct) category estimates the optical trap's stiffness by measuring the changes in momentum of scattered trapping light.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of methods to calibrate the stiffness of a single-beam gradient-force optical tweezers over various laser trapping powers

2014

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Abstract. Optical tweezers have become an important instrument in force measurements associated with various physical, biological, and biophysical phenomena. Quantitative use of optical tweezers relies on accurate calibration of the stiffness of the optical trap. Using the same optical tweezers platform operating at 1064 nm and beads with two different diameters, we present a comparative study of viscous drag force, equipartition theorem, Boltzmann statistics, and power spectral density (PSD) as methods in calibrating the stiffness of a single beam gradient force optical trap at trapping laser powers in the range of 0.05 to 1.38 W at the focal plane. The equipartition theorem and Boltzmann statistic methods demonstrate a linear stiffness with trapping laser powers up to 355 mW, when used in conjunction with video position sensing means. The PSD of a trapped particle's Brownian motion or measurements of the particle displacement against known viscous drag forces can be reliably used for stiffness calibration of an optical trap over a greater range of trapping laser powers. Viscous drag stiffness calibration method produces results relevant to applications where trapped particle undergoes large displacements, and at a given position sensing resolution, can be used for stiffness calibration at higher trapping laser powers than the PSD method.

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Three-dimensional optical force field on a Chinese hamster ovary cell in a fiber-optical dual-beam trap

Cited by 42 publications

References 12 publications

Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications

Reconfigurable microfluidic integration of a dual-beam laser trap with biomedical applications

Microfluidic electromanipulation with capacitive detection for the mechanical analysis of cells

Comparative study of methods to calibrate the stiffness of a single-beam gradient-force optical tweezers over various laser trapping powers

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