Three‐Dimensional Nanostructured Substrates toward Efficient Capture of Circulating Tumor Cells

Wang, Shutao; Wang, Hao; Jiao, Jing; Chen, Kuan‐Ju; Owens, Gwen���E.; Kamei, Kazuhito; Sun, Jing; Sherman, David���J.; Behrenbruch, Christian���P.; Wu, Hong; Tseng, Hsian-Rong

doi:10.1002/ange.200901668

Cited by 111 publications

(62 citation statements)

References 38 publications

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“…As shown in Figures 4a, an average capture efficiency of 80.9 % was obtained in lysed blood, indicating that cell-imprinted gelatin grafted with anti-EpCAM exhibits sensitive and specific performance for capturing EpCAM-positive cells. A capture efficiency of 52 % was obtained in whole blood sample, which was comparable to other static CTC detection devices, including such as vertically oriented silicon nanopillars, 56 horizontally packed electrospun TiO 2 nanofiber, 53 and fractal nanostructured Au Interfaces. 22 The decreased capture efficiency in whole blood was possibly caused by the blockage from red blood cells and the reduced interaction chance between conjugate and the target cells.…”

Section: Template Preparation and Stamp Fabricationmentioning

confidence: 62%

Near-Infrared Light-Responsive Hydrogel for Specific Recognition and Photothermal Site-Release of Circulating Tumor Cells

et al. 2016

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Isolation of single circulating tumor cells (CTCs) from patients is a very challenging technique that may promote the process of individualized antitumor therapies. However, there exist few systems capable of highly efficient capture and release of single CTCs with high viability for downstream analysis and culture. Herein, we designed a near-infrared (NIR) light-responsive substrate for highly efficient immunocapture and biocompatible site-release of CTCs by a combination of the photothermal effect of gold nanorods (GNRs) and a thermoresponsive hydrogel. The substrate was fabricated by imprinting target cancer cells on a GNR-pre-embedded gelatin hydrogel. Micro/nanostructures generated by cell imprinting produce artificial receptors for cancer cells to improve capture efficiency. Temperature-responsive gelatin dissolves rapidly at 37 °C; this allows bulk recovery of captured CTCs at physiological temperature or site-specific release of single CTCs by NIR-mediated photothermal activation of embedded GNRs. Furthermore, the system has been applied to capture, individually release, and genetically analyze CTCs from the whole blood of cancer patients. The multifunctional NIR-responsive platform demonstrates excellent performance in capture and site-release of CTCs with high viability, which provides a robust and versatile means toward individualized antitumor therapies and also shows promising potential for dynamically manipulating cell-substrate interactions in vitro.

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Section: Template Preparation and Stamp Fabricationmentioning

confidence: 62%

Near-Infrared Light-Responsive Hydrogel for Specific Recognition and Photothermal Site-Release of Circulating Tumor Cells

et al. 2016

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“…Immunoassay-based detection including micro/nanostructured surfaces or immune-magnetic beads is more commonly used which rely primarily on antigen-antibody affinity by recognizing tumor-specific markers especially epithelial cell adhesion molecules (EpCAM) to capture targeted cancer cells. [20][21][22] Micro/nanostructures-based approaches often use complicated structures, such as arrays of microposts, 23 nanopillars, 24,25 nanowires, 26 nanofibers, 27 and nanodots 28 to enhance the interactions with cells, improving the capture efficiency to target cells. Alternatively, benefiting from easy manipulation, fast magnetic response and high capture efficiency, immunomagnetic-based assays have been widely applied to CTCs detection.…”

Section: Introductionmentioning

confidence: 99%

Biotin-Triggered Decomposable Immunomagnetic Beads for Capture and Release of Circulating Tumor Cells

Xie

Wang

et al. 2015

ACS Appl. Mater. Interfaces

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Isolation of rare, pure, and viable circulating tumor cells (CTCs) provides a significant insight in early cancer diagnosis, and release of captured CTCs without damage for ex vivo culture may offer an opportunity for personalized cancer therapy. In this work, we described a biotin-triggered decomposable immunomagnetic system, in which peptide-tagged antibody designed by chemical conjugation was specifically immobilized on engineered protein-coated magnetic beads. The interaction between peptide and engineered protein can be reversibly destroyed by biotin treatment, making capture and release of CTCs possible. Furthermore, the peptide could mediate multiple antibodies' coimmobilization on engineered protein-coated magnetic beads, by which capture efficiency for CTCs was obviously improved. Quantitative results showed that 70% of captured cells could be released by biotin addition, and 85% of released cells remained viable. In addition, 79% of cancer cells spiked in human whole blood were captured and could also be successfully released for culture. Finally, immunomagnetic beads simultaneously loaded with anti-EpCAM, anti-HER2, and anti-EGFR were successfully applied to isolate and detect CTCs in 17 cancer patients' peripheral blood samples, and 2-215 CTCs were identified with high purity. These results suggest that our method is reliable and has great potential in CTC detection for CTC-based molecular profiling, diagnosis, and therapy.

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“…In addition, as cells move through the microchannel and fail to interact with the antibody-coated surface, we observed cells beginning to clump, further decreasing the possibility of capture. In order to increase cell-antibody interactions, one could incorporate a herringbone design to promote mixing (Mittal et al 2012;Stott et al 2010;Wang et al 2011a) or could increase the effective surface area, for example, by incorporating antibodyfunctionalized microposts in addition to a functionalized solid surface (Gleghorn et al 2010;Nagrath et al 2007;Wang et al 2009). …”

Section: Antibody-functionalized Microfluidic Devicesmentioning

confidence: 99%

A microfluidic method for the selection of undifferentiated human embryonic stem cells and in situ analysis

Jabart

Rangarajan

Lieu

et al. 2014

Microfluid Nanofluid

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Three‐Dimensional Nanostructured Substrates toward Efficient Capture of Circulating Tumor Cells

Cited by 111 publications

References 38 publications

Near-Infrared Light-Responsive Hydrogel for Specific Recognition and Photothermal Site-Release of Circulating Tumor Cells

Near-Infrared Light-Responsive Hydrogel for Specific Recognition and Photothermal Site-Release of Circulating Tumor Cells

Biotin-Triggered Decomposable Immunomagnetic Beads for Capture and Release of Circulating Tumor Cells

A microfluidic method for the selection of undifferentiated human embryonic stem cells and in situ analysis

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