Three-Dimensional Morphological Analysis of Antigen-Antibody Reaction in Hepatic Sinusoids Preserved in Hypothermic UW Solution

Sasaki, Katsunori; Johkura, Kohei; Ogiwara, N.; Liang, Yan; Cui, Li; Teng, Ruifeng; Okouchi, Yasumitsu; Asanuma, Kazuhiko; Ishida, Osamu; Maruyama, Kazuo

doi:10.1006/cryo.2001.2316

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…2 mm) and examined as described. 25) Surgical Procedure for Isolated Hepatic Perfusion Specific pathogen free, 8-to 10-week-old male Wistar rats (Japan SLC, Shizuoka, Japan) were used throughout the study. All the animal experiments were performed according to our institutional guidelines for the care and use of laboratory animals.…”

Section: Purification and Physical Characterization Of Sendai Virus Vmentioning

confidence: 99%

Sendai Virus-Mediated Gene Delivery into Hepatocytes via Isolated Hepatic Perfusion

Fujita

Okabe

Harada

et al. 2006

Biological & Pharmaceutical Bulletin

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The recombinant Sendai virus vector is a promising tool for human gene therapy, capable of inducing highlevel expression of therapeutic genes in tissue cells in situ. The target tissues include airway epithelium, blood vessels, skeletal muscle, retina and the central nervous system, but application to hepatic tissues has not yet been achieved, because direct intraportal injection of the vector is not feasible. We report an efficient and harmless procedure of gene delivery by recombinant Sendai virus into rat parenchymal hepatocytes, based on isolated hepatic perfusion with controlled inflow. Critical parameters for successful hepatic gene delivery are a brief preperfusion period (25°C, 5 min); appropriate vector concentration in the perfusate (10 7 pfu/ml); moderate portal vein pressure (12 mmHg) and a brief hyperthermic postperfusion period (42°C, 5 min). Under these optimized conditions, marker genes were expressed in most parenchymal hepatocytes without significant damage to hepatic tissues. Furthermore, expression of the marker genes was undetectable in nonhepatic tissues, including the gonads, indicating that this approach strictly targets hepatic tissues and thus offers good clinical potential for human gene therapy.

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Section: Purification and Physical Characterization Of Sendai Virus Vmentioning

confidence: 99%

Sendai Virus-Mediated Gene Delivery into Hepatocytes via Isolated Hepatic Perfusion

Fujita

Okabe

Harada

et al. 2006

Biological & Pharmaceutical Bulletin

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Expression of Intercellular Adhesion Molecule-1 (ICAM-1) During Ischemia–Reperfusion in Human Liver Tissue Allograft: Image Analysis by Confocal Laser Scanning Microscopy

Benkoël

Dodero

Hardwigsen³

et al. 2003

Digestive Diseases and Sciences

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We studied and quantified the effect of ischemia-reperfusion on expression of intercellular adhesion molecule-1 (ICAM-1) on sinusoidal endothelial cells and hepatocyte plasma membranes by means of confocal laser scanning microscopy imaging. We found that ischemia induced an increase in ICAM-1 expression on sinusoidal endothelial cells and hepatocytes. After reperfusion, ICAM-1 expression was increased on sinusoidal endothelial cells, whereas it was unmodified on hepatocytes. On the other hand, ICAM-1 expression was not correlated to ischemia-reperfusion liver injury. Therefore, hepatocellular ischemia-reperfusion injury could be induced by other causes than immune-mediated damages.

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Three-Dimensional Morphological Analysis of Antigen-Antibody Reaction in Hepatic Sinusoids Preserved in Hypothermic UW Solution

Cited by 2 publications

References 13 publications

Sendai Virus-Mediated Gene Delivery into Hepatocytes via Isolated Hepatic Perfusion

Sendai Virus-Mediated Gene Delivery into Hepatocytes via Isolated Hepatic Perfusion

Expression of Intercellular Adhesion Molecule-1 (ICAM-1) During Ischemia–Reperfusion in Human Liver Tissue Allograft: Image Analysis by Confocal Laser Scanning Microscopy

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