Three-Dimensional Migration of Neutrophils Through an Electrospun Nanofibrous Membrane

Jin, Songwan; Park, Tae-Min; Kim, Cho-Hee; Le, Binh Duong; Jeong, Young Hun; Kwak, Jong‐Young; Yoon, Sik

doi:10.2144/000114297

Cited by 5 publications

(5 citation statements)

References 28 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Technically, the PCL-NM can be easily assembled into the two-layer system, which mimics multilayers of cells in tissue. Moreover, PCL nanofibers are inert and biocompatible in immune cell culture because the adhesion of DCs to PCL nanofibers does not affect their activation status [ 12 , 13 ]. Finally, 3D migration to surrounding bacteria and phagocytosis processes in the PCL-NM can be visualized though a live cell-imaging setup.…”

Section: Discussionmentioning

confidence: 99%

“…For optimal cellular infiltration, we developed a hybrid electrospun poly(ε-caprolactone) (PCL)-nanofibrous membrane (NM) (PCL-NM) consisting of nano- as well as submicron-scale fibers and we used the PCL-NM to mimic the naturally occurring crosstalk between immune and cancer cells [ 11 ]. Additionally, we demonstrated that neutrophils migrate against interleukin (IL)-8 in an electrospun PCL-NM [ 12 ]. PCL is a Food and Drug Administration (FDA)-approved biocompatible and biodegradable aliphatic polyester [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bacterial Infection-Mimicking Three-Dimensional Phagocytosis and Chemotaxis in Electrospun Poly(ε-caprolactone) Nanofibrous Membrane

et al. 2021

Self Cite

View full text Add to dashboard Cite

In this study, we developed a three-dimensional (3D) in vitro infection model to investigate the crosstalk between phagocytes and microbes in inflammation using a nanofibrous membrane (NM). Poly(ε-caprolactone) (PCL)-NMs (PCL-NMs) were generated via electrospinning of PCL in chloroform. Staphylococcus aureus and phagocytes were able to adhere to the nanofibers and phagocytes engulfed S. aureus in the PCL-NM. The migration of phagocytes to S. aureus was evaluated in a two-layer co-culture system using PCL-NM. Neutrophils, macrophages and dendritic cells (DCs) cultured in the upper PCL-NM layer migrated to the lower PCL-NM layer containing bacteria. DCs migrated to neutrophils that cultured with bacteria and then engulfed neutrophils in two-layer system. In addition, phagocytes in the upper PCL-NM layer migrated to bacteria-infected MLE-12 lung epithelial cells in the lower PCL-NM layer. S. aureus-infected MLE-12 cells stimulated the secretion of tumor necrosis factor-α and IL-1α in 3D culture conditions, but not in 2D culture conditions. Therefore, the PCL-NM-based 3D culture system with phagocytes and bacteria mimics the inflammatory response to microbes in vivo and is applicable to the biomimetic study of various microbe infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bacterial Infection-Mimicking Three-Dimensional Phagocytosis and Chemotaxis in Electrospun Poly(ε-caprolactone) Nanofibrous Membrane

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…NF was fabricated by a previously reported electrospinning method [ 20 , 21 ]. Briefly, nanofibers were produced by diluting high molecular weight (80,000 MW) polycaprolactone (Sigma-Aldrich, St. Louis, MO, USA) at a concentration of 17% in a 1:1 mixture of chloroform (99.5%, Samchun Pure Chemical Co., Ltd., Seoul, Korea) and dimethylformamide (Sigma-Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing

Park¹,

Kang

Jang

et al. 2017

IJMS

Self Cite

View full text Add to dashboard Cite

In general, a drug candidate is evaluated using 2D-cultured cancer cells followed by an animal model. Despite successful preclinical testing, however, most drugs that enter human clinical trials fail. The high failure rates are mainly caused by incompatibility between the responses of the current models and humans. Here, we fabricated a cancer microtissue array in a multi-well format that exhibits heterogeneous and batch-to-batch structure by continuous deposition of collagen-suspended Hela cells on a fibroblast-layered nanofibrous membrane via inkjet printing. Expression of both Matrix Metalloproteinase 2 (MMP2) and Matrix Metalloproteinase 9 (MMP9) was higher in cancer microtissues than in fibroblast-free microtissues. The fabricated microtissues were treated with an anticancer drug, and high drug resistance to doxorubicin occurred in cancer microtissues but not in fibroblast-free microtissues. These results introduce an inkjet printing fabrication method for cancer microtissue arrays, which can be used for various applications such as early drug screening and gradual 3D cancer studies.

show abstract

“…This technique has the advantages to produce homogeneous fiber sizes. Polymers such as polycaprolactone have been used in order to study immune cells migration through a fibrous membrane of various density (Jin et al 2015 ). The 2D structures are then used as a scaffold, and can be coated with collagen for more physiological properties of basement membrane.…”

Section: Migration Of Immune Cells In Diverse Microfabricated Geometriesmentioning

confidence: 99%

Characterization of immune cell migration using microfabrication

Vesperini

Montalvo

et al. 2021

Biophys Rev

View full text Add to dashboard Cite

The immune system provides our defense against pathogens and aberrant cells, including tumorigenic and infected cells. Motility is one of the fundamental characteristics that enable immune cells to find invading pathogens, control tissue damage, and eliminate primary developing tumors, even in the absence of external treatments. These processes are termed “immune surveillance.” Migration disorders of immune cells are related to autoimmune diseases, chronic inflammation, and tumor evasion. It is therefore essential to characterize immune cell motility in different physiologically and pathologically relevant scenarios to understand the regulatory mechanisms of functionality of immune responses. This review is focused on immune cell migration, to define the underlying mechanisms and the corresponding investigative approaches. We highlight the challenges that immune cells encounter in vivo, and the microfabrication methods to mimic particular aspects of their microenvironment. We discuss the advantages and disadvantages of the proposed tools, and provide information on how to access them. Furthermore, we summarize the directional cues that regulate individual immune cell migration, and discuss the behavior of immune cells in a complex environment composed of multiple directional cues.

show abstract

Three-Dimensional Migration of Neutrophils Through an Electrospun Nanofibrous Membrane

Cited by 5 publications

References 28 publications

Bacterial Infection-Mimicking Three-Dimensional Phagocytosis and Chemotaxis in Electrospun Poly(ε-caprolactone) Nanofibrous Membrane

Bacterial Infection-Mimicking Three-Dimensional Phagocytosis and Chemotaxis in Electrospun Poly(ε-caprolactone) Nanofibrous Membrane

Fabrication of In Vitro Cancer Microtissue Array on Fibroblast-Layered Nanofibrous Membrane by Inkjet Printing

Characterization of immune cell migration using microfabrication

Contact Info

Product

Resources

About