“…, handing drop insertion ( Tung et al, 2011 ; Zhao et al, 2019 ; Fu et al, 2021 ; Liu et al, 2021 ), including magnetic levitation ( Kim et al, 2013 ; Tseng et al, 2015 ; Ge et al, 2018 ), and nonadhesive surface ( Friedrich et al, 2009 ; Liao et al, 2019 )] were developed to address several applications. Products such as the ultra-low attachment (ULA) plates are available ( Vinci et al, 2012 ; Raghavan et al, 2016 ) along with other techniques for 3D spheroidal culture development, for example, microfabrication ( Lee J. M et al, 2018 ; Park et al, 2018 ; Ganguli et al, 2021 ; Sun et al, 2021 ), soft lithography ( Cha et al, 2017 ; Kuo et al, 2017 ), and floating methods ( Vadivelu et al, 2015 ; Kim et al, 2021 ) among others. Despite the widespread interest, the current culture methods have several limitations, including nonuniformity of spheroid formation, irregular sphericity, short-term cultures, evaporative loss of culture media, and limited volume (size of spheroids) of culture ( Shi et al, 2018 ; De Souza Moraes et al, 2020 ; Han et al, 2021 ).…”