Three‐dimensional microarray platform applied to single nucleotide polymorphism analysis

Stimpson, Donald I.; Knepper, Sheila M.; Shida, Miho M.; Obata, Kimimichi; Tajima, Hideji

doi:10.1002/bit.20087

Cited by 12 publications

(3 citation statements)

References 6 publications

(7 reference statements)

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“…By detecting only the pillar surfaces which are several hundred microns from the base, background noise is removed from the microarray scan. A 3-D microarray which is markedly different from the above-mentioned approaches involves immobilizing oligonucleotide probes onto a single thread instead of a planar substrate (Stimpson et al, 2004). The thread is subsequently wound around a core to form a compact, high-density SNP detection platform.…”

Section: -D Microarraymentioning

confidence: 99%

Multiplexing Capabilities of Biosensors for Clinical Diagnostics

Ng¹,

Chong²

2011

Biosensors for Health, Environment and Biosecurity

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Section: -D Microarraymentioning

confidence: 99%

Multiplexing Capabilities of Biosensors for Clinical Diagnostics

Ng¹,

Chong²

2011

Biosensors for Health, Environment and Biosecurity

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“…The last decade has seen the rapid development of microarray technology and its successful application in genomic and enzymatic analyses [1][2][3]. A microarray is a two-dimensional (2D) grid of a large number of unique materials deposited at known or defined locations on a flat substrate.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a three-dimensional tissue model microarray using laser foaming of a gas-impregnated biodegradable polymer

Ock¹,

Li²

2014

Biofabrication

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A microarray containing three-dimensional (3D) tissue models is a promising substitute for the two-dimensional (2D) cell-based microarrays currently available for high throughput, tissue-based biomedical assays. A cell culture microenvironment similar to in vivo conditions could be achieved with biodegradable porous scaffolds. In this study, a laser foaming technique is developed to create an array of micro-scale 3D porous scaffolds. The effects of major process parameters and the morphology of the resulting porous structure were investigated. For comparison, cell culture studies were conducted with both foamed and unfoamed samples using T98G cells. The results show that by laser foaming gas-impregnated polylactic acid it is possible to generate an array of inverse cone shaped wells with porous walls. The size of the foamed region can be controlled with laser power and exposure time, while the pore size of the scaffold can be manipulated with the saturation pressure. T98G cells grow well in the foamed scaffolds, forming clusters that have not been observed in 2D cell cultures. Cells are more viable in the 3D scaffolds than in the 2D cell culture cases. The 3D porous microarray could be used for parallel studies of drug toxicity, guided stem cell differentiation, and DNA binding profiles.

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“…Multiplexed assays are thus crucial to complement advances in genomics and proteomics to allow a large number of nucleic acids and proteins to be rapidly screened. Oligonucleotide microarrays − and protein arrays − can handle a high degree of multiplexed detection using spatially resolved measurements, but the experimental equipment and detection systems are not convenient to use on a routine basis. Lacking real-time monitoring capability, these array technologies cannot be used for biological sample imaging.…”

mentioning

confidence: 99%

Dual-Luminophore-Doped Silica Nanoparticles for Multiplexed Signaling

2004

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We have synthesized dual-luminophore-doped silica nanoparticles for multiplexed signaling in bioanalysis. Two luminophores, Tris(2,2'-bipyridyl)osmium(II)bis(hexafluorophosphate) (OsBpy) and Tris(2,2'-bipyridyl)dichlororuthenium(II)hexahydrate (RuBpy), were simultaneously entrapped inside silica nanoparticles at precisely controlled ratios, with desirable sizes and required surface functionality. Single-wavelength excitation with dual emission endows the nanoparticles with optical encoding capability for rapid and high-throughput multiplexed detection. The nanoparticles can be prepared with sizes ranging from a few nanometers to a few hundred nanometers, with specific ratios of luminescence intensities at two well-resolved wavelengths and with excellent reproducibility. These nanoparticles also possess unique properties of high signal amplification, excellent photostability, and easy surface bioconjugation for highly sensitive measurements when used as signaling markers. A simplified ligand binding system using avidin-biotin and an application extension to immunoassays have been explored, demonstrating the potential use of these easily obtainable bioconjugated nanoparticles for multiplexed signaling and bioassays.

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Three‐dimensional microarray platform applied to single nucleotide polymorphism analysis

Cited by 12 publications

References 6 publications

Multiplexing Capabilities of Biosensors for Clinical Diagnostics

Multiplexing Capabilities of Biosensors for Clinical Diagnostics

Fabrication of a three-dimensional tissue model microarray using laser foaming of a gas-impregnated biodegradable polymer

Dual-Luminophore-Doped Silica Nanoparticles for Multiplexed Signaling

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