The abnormal climate change has made the reduction of CO 2 emission that received worldwide attention. The integration of CO 2 capture-sequestration application for enhanced oil recovery (EOR) technology will be the new trend. Several scholars have applied microfluidics in CO 2 capture, oil and gas analysis, and CO 2 sequestration. The mass transfer process for CO 2 capture can be intensified owing to the large specific surface/ volume ratio and high contact area in microchannels. The small amount of feeding volumes of oil and gas samples and the quick response for the analysis make the microfluidics a promising tool for the oil and gas analysis. Moreover, microfluidics can reveal the transport mechanism at microscale for multiphase interfacial phenomena in microchannels within porous media during the CO 2 flooding process in line with the pressure, temperature, and material properties of the rock within the oil reservoir. This chapter will elaborate the progress of the application of microfluidic technology in the utilization of CO 2, including the mechanism of mass transfer for CO 2 in microreactors, the advantages of microfluidics in oil and gas analysis, and the fundamentals of microfluidics in CO 2 flooding, oil recovery improvement, and CO 2 sequestration.