Three-dimensional levitation culture improves in-vitro growth of secondary follicles in bovine model

Antonino, Deize de Cássia; Soares, Michel S.; Júnior, Jairo de Melo; Alvarenga, Paula Batista de; Mohallem, Renata de Freitas Ferreira; Rocha, Carina Diniz; Vieira, Luis Alberto; Souza, Aline Gomes de; Beletti, Marcelo Emı́lio; Alves, Bênner Geraldo; Jacomini, José Octávio; Goulart, Luíz Ricardo; Alves, Kele Amaral

doi:10.1016/j.rbmo.2018.11.013

Cited by 26 publications

(23 citation statements)

References 33 publications

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“…It was observed that the 3D culture system enhanced development of secondary follicles when compared to a two‐dimensional control treatment (follicles individually culture in 24‐well plate). Also, after in vitro maturation, the 3D system improved oocyte viability and meiotic resumption when compared to the control group (Antonino et al, ).…”

Section: Nanotechnologymentioning

confidence: 98%

“…It was observed that the 3D culture system enhanced development of secondary follicles when compared to a two-dimensional control treatment (follicles individually culture in 24-well plate). Also, after in vitro maturation, the 3D system improved oocyte viability and meiotic resumption when compared to the control group (Antonino et al, 2019 do not need to be surgically retrieved and present similarities regarding morphology and maturation profiles (Barkalina et al, 2016). Characterization of sperm and identification of potential targets, such as proteins related to acrosome integrity (Cross & Watson, 1994) and genes linked to freezability (Vilagran et al, 2015), make the use of nanoparticles interesting for applications in studies involving sperm enrichment and sorting (Feugang, 2017).…”

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confidence: 99%

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Applications of omics and nanotechnology to improve pig embryo production in vitro

Lucas

Chen

Seixas

et al. 2019

Molecular Reproduction Devel

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An appropriate environment to optimize porcine preimplantation embryo production in vitro is required as genetically modified pigs have become indispensable for biomedical research and agriculture. To provide suitable culture conditions, omics technologies have been applied to elucidate which metabolic substrates and pathways are involved during early developmental processes. Metabolomic profiling and transcriptional analysis comparing in vivo‐ and in vitro‐derived embryos have demonstrated the important role of amino acids during preimplantation development. Transcriptional profiling studies have been helpful in assessing epigenetic reprogramming agents to allow for the correction of gene expression during the cloning process. Along with this, nanotechnology, which is a highly promising field, has allowed for the use of engineered nanoplatforms in reproductive biology. A growing number of studies have explored the use of nanoengineered materials for sorting, labeling, and targeting purposes; which demonstrates their potential to become one of the solutions for precise delivery of molecules into gametes and embryos. Considering the contributions of omics and the recent progress in nanoscience, in this review, we focused on their emerging applications for current in vitro pig embryo production systems to optimize the generation of genetically modified animals.

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Section: Nanotechnologymentioning

confidence: 98%

mentioning

confidence: 99%

Applications of omics and nanotechnology to improve pig embryo production in vitro

Lucas

Chen

Seixas

et al. 2019

Molecular Reproduction Devel

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“…Porcine follicles showed high viability and good morphology after a 10 days-culture, while oocyte quality needs to be assessed [105]. The third has been recently published by Antonino and colleagues [106], who cultured bovine preantral follicles in a 3D levitation system based on the utilization of More recently, three new approaches have been reported in the literature. The first is based on the decellularization of human ovary.…”

Section: The New Ovariesmentioning

confidence: 99%

“…Porcine follicles showed high viability and good morphology after a 10 days-culture, while oocyte quality needs to be assessed [105]. The third has been recently published by Antonino and colleagues [106], who cultured bovine preantral follicles in a 3D levitation system based on the utilization of magnetic nanoparticles. Briefly, follicles were individually incubated in microtubes with culture medium and nanoparticles, and then transferred to a 24-well plate covered with a lid insert and a magnetic drive to allow levitation after 1 h of magnetic treatment and cultured for 16 days.…”

Section: The New Ovariesmentioning

confidence: 99%

Technologies for the Production of Fertilizable Mammalian Oocytes

et al. 2019

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Women affected by ovarian pathologies or with cancer can usually preserve fertility by egg/embryo freezing. When oocyte retrieval is not feasible, the only option available is ovarian tissue cryopreservation and transplantation. The culture of follicles isolated from fresh or cryopreserved ovaries is considered still experimental, although this procedure is considered safer, because the risk of unintentional spreading of cancer cells eventually present in cryopreserved tissue is avoided. Animal and human small follicles can be cultured in vitro, but standardized protocols able to produce in vitro grown oocytes with the same developmental capacity of in vivo grown oocytes are not available yet. In fact, the different sizes of follicles and oocytes, the hormonal differences existing between mono- (e.g., human, goat, cow, and sheep) and poly-ovulatory (rodents and pig) species, and the incomplete identification of the mechanisms regulating the oocyte–follicle and follicle–ovary interrelationships affect the outcome of in vitro culture. From all these attempts, however, new ideas arise, and the goal of assuring the preservation of female reproductive potential appears a more realistic possibility. This review surveys and discusses advances and challenges of these technologies that, starting from a simple attempt, are now approaching the biosynthesis of a functional engineered ovary.

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“…Neste sentido, a interação entre os dois compartimentos celulares têm sido investigada por meio do desenvolvimento de sistemas de cultivo de ovócitos com: monocamadas de células do cúmulus ou granulosa (Hashimoto et al, 1998;Huang e Wells, 2010), fragmentos da parede folicular e bloqueadores de meiose (cilostamida) (Correia et al, 2019) e associados com matriz extracelular de colágeno em sistema tridimensional (Combelles et al, 2005;Ma et al, 2007). O sistema de cultivo tridimensional permite que linhagens celulares, células primárias e células-tronco possam ser colocadas em conjunto, formando estruturas densas em uma matriz extracelular que favorece a manutenção da morfologia, sobrevivência, migração, proliferação, e comunicação intercelular similares aos observados em tecidos (Haisler et al, 2013;Antonino et al, 2019).…”

Section: Introductionunclassified

Maturação ovocitária in vitro avaliada conforme sistemas de cultivo bidimensional e tridimensional

Júnior¹,

Pires²

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The success of in vitro embryo production is directly linked to the quality of the oocytes and the conditions of in vitro culture. In the first stage of production called maturation, which refers to the in vitro culture of immature oocytes, the oocytes are selected and receive stimuli, follicular growth and composition from the medium in which they are inserted for the evolution of meiosis, involving nuclear and cytoplasmic changes essential for fertilization and subsequent embryonic development. However, productivity rates remain unchanged and further studies should be conducted in order to improve the quality of oocytes after the in vitro maturation phase. Thus, this study was conducted with two different experiments with the objective of: 1-Assessing the maturation of bovine oocytes with or without cumulus cells in three-dimensional in vitro culture systems using collagen matrix and comparing the traditional two-dimensional system; 2-Compare the methods of visual evaluation using an inverted microscope and fluorescence microscopy after the oocyte maturation process. The percentage of oocytes in the germinal vesicle was higher in those without cumulus cells. The oocytes with cumulus cells in a three-dimensional culture system showed a lower percentage of oocytes in metaphase I. Additionally, metaphase II rates were higher in those with cumulus cells regardless of the culture system. In addition, the percentage of ruptured oocytes was higher in the cumulus cells in 3D culture compared to the others. Different approaches were used to evaluate the oocyte chromatin post-maturation. In the evaluation by an inverted microscope, the parameters presence of the polar corpuscle and extrusion were evaluated. In general, the cumulus cells in 3D system showed a higher percentage of oocytes with polar body extrusion. The parameters analyzed germinal vesicle (fluorescence microscopy) versus absence of polar corpuscle (inverted microscope) and metaphase II (fluorescence microscopy) versus presence of polar corpuscle (inverted microscope) were compared between the approaches used: fluorescence microscopy versus inverted microscope. In general, the percentage of oocytes in the germinal vesicle (fluorescence microscopy) was lower compared to the proportion of oocytes observed with the absence of a polar corpuscle (inverted microscope). On the other hand, the percentage of oocytes in metaphase II (fluorescence microscopy) was higher than those identified with the presence of a polar corpuscle (inverted microscope). In summary, considering the results obtained through the analysis of fluorescence microscopy (elective as gold standard), the resumption of meiosis after the in vitro cultivation of oocytes with cumulus cells was similar between the 2D and 3D systems. Within the same evaluated system (2D or 3D), the absence of cumulus cells compromised the resumption of meiosis. In addition, oocytes matured in the 3D system showed a higher occurrence of damage in the pellucid zone.

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Three-dimensional levitation culture improves in-vitro growth of secondary follicles in bovine model

Cited by 26 publications

References 33 publications

Applications of omics and nanotechnology to improve pig embryo production in vitro

Applications of omics and nanotechnology to improve pig embryo production in vitro

Technologies for the Production of Fertilizable Mammalian Oocytes

Maturação ovocitária in vitro avaliada conforme sistemas de cultivo bidimensional e tridimensional

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