Three-Dimensional In Vitro Models in Glioma Research - Focus on Spheroids

Jensen, Stine Skov; Aaberg-Jessen, Charlotte; Jakobsen, Ida Pind; Hermansen, Simon Kjær; Nissen, Søren Kabell; Kristensen, Bjarne Winther

doi:10.5772/22196

Glioma - Exploring Its Biology and Practical Relevance 2011

DOI: 10.5772/22196

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Three-Dimensional In Vitro Models in Glioma Research - Focus on Spheroids

Stine Skov Jensen¹,

Charlotte Aaberg-Jessen²,

Ida Pind Jakobsen³

et al.

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2018

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The therapeutic potential of polymersomes loaded with 225Ac evaluated in 2D and 3D in vitro glioma models

Kruijff

Meer

Windmeijer

et al. 2018

European Journal of Pharmaceutics and Biopharmaceutics

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Alpha emitters have great potential in targeted tumour therapy, especially in destroying micrometastases, due to their high linear energy transfer (LET). To prevent toxicity caused by recoiled daughter atoms in healthy tissue, alpha emitters like Ac can be encapsulated in polymeric nanocarriers (polymersomes), which are capable of retaining the daughter atoms to a large degree. In the translation to a (pre-)clinical setting, it is essential to evaluate their therapeutic potential. As multicellular tumour spheroids mimic a tumour microenvironment more closely than a two-dimensional cellular monolayer, this study has focussed on the interaction of the polymersomes with U87 human glioma spheroids. We have found that polymersomes distribute themselves throughout the spheroid after 4 days which, considering the long half-life ofAc (9.9 d) (Vaidyanathan and Zalutsky, 1996), allows for irradiation of the entire spheroid. A decrease in spheroidal growth has been observed upon the addition of only 0.1 kBq Ac, an effect which was more pronounced for theAc in polymersomes than when only coupled to DTPA. At higher activities (5 kBq), the spheroids have been found to be destroyed completely after two days. We have thus demonstrated that Ac containing polymersomes effectively inhibit tumour spheroid growth, making them very promising candidates for future in vivo testing.

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The therapeutic potential of polymersomes loaded with 225Ac evaluated in 2D and 3D in vitro glioma models

Kruijff

Meer

Windmeijer

et al. 2018

European Journal of Pharmaceutics and Biopharmaceutics

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show abstract

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Three-Dimensional In Vitro Models in Glioma Research - Focus on Spheroids

Cited by 1 publication

References 162 publications

The therapeutic potential of polymersomes loaded with 225Ac evaluated in 2D and 3D in vitro glioma models

The therapeutic potential of polymersomes loaded with 225Ac evaluated in 2D and 3D in vitro glioma models

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