Three-Dimensional in Situ Temperature Measurement in Microsystems Using Brownian Motion of Nanoparticles

Chung, Kwanghun; Cho, Jae Kyu; Park, Edward S.; Breedveld, Victor; Lu, Hang

doi:10.1021/ac802031j

Cited by 23 publications

(14 citation statements)

References 39 publications

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“…To do so, we avoided using back focal plane interferometry to record and track bead positions, and instead used a high-speed camera (recording rate: 4000 fps) and custom video tracking software for this purpose. We calibrated the assay sample temperature with 50.5 C precision using several independent noncontact methods, including measuring bead diffusion (20)(21)(22), performing fixed-point calibration, and measuring the thermal shift of the peak of emission spectra for Cd-Se and Cd-Te quantum dot fluorescence (23). Further details of the temperature control and calibration will be published elsewhere.…”

Section: Temperature Controlmentioning

confidence: 99%

The Effect of Temperature on Microtubule-Based Transport by Cytoplasmic Dynein and Kinesin-1 Motors

Hong

Takshak

Osunbayo

et al. 2016

Biophysical Journal

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Cytoplasmic dynein and kinesin are both microtubule-based molecular motors but are structurally and evolutionarily unrelated. Under standard conditions, both move with comparable unloaded velocities toward either the microtubule minus (dynein) or plus (most kinesins) end. This similarity is important because it is often implicitly incorporated into models that examine the balance of cargo fluxes in cells and into models of the bidirectional motility of individual cargos. We examined whether this similarity is a robust feature, and specifically whether it persists across the biologically relevant temperature range. The velocity of mammalian cytoplasmic dynein, but not of mammalian kinesin-1, exhibited a break from simple Arrhenius behavior below 15°C-just above the restrictive temperature of mammalian fast axonal transport. In contrast, the velocity of yeast cytoplasmic dynein showed a break from Arrhenius behavior at a lower temperature (∼8°C). Our studies implicate cytoplasmic dynein as a more thermally tunable motor and therefore a potential thermal regulator of microtubule-based transport. Our theoretical analysis further suggests that motor velocity changes can lead to qualitative changes in individual cargo motion and hence net intracellular cargo fluxes. We propose that temperature can potentially be used as a noninvasive probe of intracellular transport.

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Section: Temperature Controlmentioning

confidence: 99%

The Effect of Temperature on Microtubule-Based Transport by Cytoplasmic Dynein and Kinesin-1 Motors

Hong

Takshak

Osunbayo

et al. 2016

Biophysical Journal

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“…The fabrication of the water circulation channels by a combination of manual cutting and lithography made it possible to combine the large channel depth (1 mm) with accurate definition of channel boundaries (see Supporting Information, Figure S1). The large depth of the circulation channels that would be difficult to achieve purely lithographically (as in some previous studies25, 26) resulted in low hydrodynamic resistance, leading to a high flow rate and uniform temperatures inside the channels. In addition, the relatively small temperature differences between the tubing lines connected to the inlets and outlets made it possible to measure the temperature in the circulation channels using thermistors inserted in the tubing lines.…”

Section: Discussionmentioning

confidence: 85%

High-Resolution Temperature−Concentration Diagram of α-Synuclein Conformation Obtained from a Single Förster Resonance Energy Transfer Image in a Microfluidic Device

et al. 2009

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We present a microfluidic device for rapid and efficient determination of protein conformations in a range of medium conditions and temperatures. The device generates orthogonal gradients of concentration and temperature in an interrogation area that fits into the field of view of an objective lens with a numerical aperture of 0.45. A single Föster resonance energy transfer (FRET) image of the interrogation area containing a dual-labeled protein provides a 100×100 point map of the FRET efficiency that corresponds to a diagram of protein conformations in the coordinates of temperature and medium conditions. The device is used to explore the conformations of α-synuclein, an intrinsically disordered protein linked to Parkinson's and Alzheimer's diseases, in the presence of a binding partner, the lipid-mimetic sodium dodecyl sulfate (SDS). The experiment provides a diagram of conformations of α-synuclein with 10,000 individual data points in a range of 21 -47 °C and 0 -2.5 mM SDS. The diagram is consistent with previous reports, but also reveals new conformational transitions that would be difficult to detect with conventional techniques. The microfluidic device can potentially be used to study other biomolecular and soft-matter systems.The crowded cellular milieu in which proteins manifest their biological activity is complex, involving numerous variables and interactions that can modulate and regulate protein structure and function. In order to understand how protein structure and function are controlled by the environment, biochemists and biophysicists routinely perform experiments where one chemical or physical parameter, such as ligand concentration or temperature, is varied, while other parameters are kept constant. The repetition of this "one-dimensional" experiment at a series of values of a second parameter can provide a two-dimensional (2D) phase diagram of the protein. However, because of the high material, labor, and time cost, the number of tested values of the second parameter is normally quite limited, and facets of the phase diagram could be missed because of insufficient resolution. The experimental workload is increased even further when additional parameters of the protein environment are varied. Therefore, new tools and techniques that enhance the speed and convenience of variation of multiple parameters are required for studies of proteins and other complex biochemical and soft-matter systems.New tools and techniques would be particularly helpful in the study of intrinsically disordered proteins (IDPs). This class of proteins exhibits little detectable structure in isolation, but often

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“…The Brownian motion of nanoparticles has been proposed as an alternative technique to perform three dimensional measurement in microsystems and avoid toxicity effects. 12 While the nanoparticles used were not toxic to the system studied in the published work, using Brownian motion is only feasible when there is no fluid motion. The requirement to measure the temperature while the device is in operation without introducing perturbations is, therefore, only fulfilled in the case of stop flow operation with biological objects not sensitive to the nanoparticles tracked.…”

Section: Introductionmentioning

confidence: 99%

Optically trapped microsensors for microfluidic temperature measurement by fluorescence lifetime imaging microscopy

et al. 2011

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The novel combination of optical tweezers and fluorescence lifetime imaging microscopy (FLIM) has been used, in conjunction with specially developed temperature-sensitive fluorescent microprobes, for the non-invasive measurement of temperature in a microfluidic device. This approach retains the capability of FLIM to deliver quantitative mapping of microfluidic temperature without the disadvantageous need to introduce a fluorescent dye that pervades the entire micofluidic system. This is achieved by encapsulating the temperature-sensitive Rhodamine B fluorophore within a microdroplet which can be held and manipulated in the microfluidic flow using optical tweezers. The microdroplet is a double bubble in which an aqueous droplet of the fluorescent dye is surrounded by an oil shell which serves both to contain the fluorophore and to provide the refractive index differential required for optical trapping of the droplet in an external aqueous medium.

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Three-Dimensional in Situ Temperature Measurement in Microsystems Using Brownian Motion of Nanoparticles

Cited by 23 publications

References 39 publications

The Effect of Temperature on Microtubule-Based Transport by Cytoplasmic Dynein and Kinesin-1 Motors

The Effect of Temperature on Microtubule-Based Transport by Cytoplasmic Dynein and Kinesin-1 Motors

High-Resolution Temperature−Concentration Diagram of α-Synuclein Conformation Obtained from a Single Förster Resonance Energy Transfer Image in a Microfluidic Device

Optically trapped microsensors for microfluidic temperature measurement by fluorescence lifetime imaging microscopy

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