Three-Dimensional Imaging of Hormone-Secreting Cells and Their Microvessel Environment in Estrogen-Induced Prolactinoma of the Rat Pituitary Gland by Confocal Laser Scanning Microscopy

Involvement of endoplasmic reticulum (ER) stress in podocyte injury induced by excessive protein accumulation

“…The 4-lm frozen sections were fixed with ethanol:ether (1:1), blocked with 2% bovine serum albumin (BSA) in phosphate-buffered saline (PBS), and incubated with purified rabbit IgG to human ORP150 (5 lg/mL) and mouse monoclonal antibody to desmin (11.5 lg/mL; DakoCytomation), followed by incubation with FITC-conjugated sheep anti-mouse IgG (1:50 dilution; DakoCytomation) and TRITC-conjugated swine anti-rabbit IgG (1:50 dilution; DAKO). The nuclei were counterstained with Hoechst Dye 33342 (Calbiochem-Novabiochem, La Jolla, CA, USA), and then the optical tomographic imaging (Z-images) for three-dimensional reconstructed images of glomeruli was performed utilizing 510 META (Carl Zeiss, Jena, Germany) [32].…”

Pituitary Hormone-producing Cells After Estradiol Application in Rat Models of Menopause

“…Type I cells contain large, irregularly shaped secretory granules (300-700 nm), type II have spherical granules (150-250 nm), and type III contain some small round granules (of 100 nm) (31). A significant increase in the number of PRL cells and their storage secretion was observed in middle-aged female rats by Takahashi et al (1984) and Itoh at al. (2001), who investigated their pituitary morphophysiology (36,37).…”

“…A significant increase in the number of PRL cells and their storage secretion was observed in middle-aged female rats by Takahashi et al (1984) and Itoh at al. (2001), who investigated their pituitary morphophysiology (36,37). Takahashi and Kawashima (1987) observed a significant age-related increase of the DNA content in PRL cells in rats of both sexes; however, this increase was more conspicuous in the females than in the males (38).…”

Inhibitory Effects of Anti-VEGF Antibody on the Growth and Angiogenesis of Estrogen-induced Pituitary Prolactinoma in Fischer 344 Rats: Animal Model of VEGF-targeted Therapy for Human Endocrine Tumors

“…their size is variable and they are excessively branched and dilated [27, 28]. In 3-D image analysis FITC-conjugate injected pituitary vessels visualized by CLSM, treatment with G6-31 provides equal prognostic inhibition as bromocriptine, a dopamine agonist, of prolactin production in lactotrophs [10, 26, 29, 35]. Furthermore antiangiogenic agents such as thalidomide [50], and a matrix metalloproteinase inhibitor [41] are reported to inhibit PRLoma growth based on a strategy to block angiogenesis.…”

“…Three dimensional (3D) imaging observation of the anterior pituitary microvessels was conducted using the fluorescein-5-isothiocyanate (FITC) conjugate gelatin method [26]. For preparation of the FITC-gelatin conjugate, the use of fluorescent dye conjugated-polypeptide was introduced using acrylamide slab gels [51].…”