Three-dimensional imaging by a microscope

Streibl, N.

doi:10.1364/josaa.2.000121

Cited by 365 publications

(291 citation statements)

References 5 publications

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“…However, even in the presence of a spherical aberration corrector, α is still of the order of tens of milliradians, much smaller than the value for α which can be used in optical microscopy (Corle & Kino 1996). As a consequence of this, many of the spatial frequencies that make up the object are not transferred (Frieden 1967;Streibl 1985;D'Alfonso et al 2008;Intaraprasonk et al 2008). …”

Section: (A) Three-dimensional Transfer and Resolution In Incoherent mentioning

confidence: 97%

Three-dimensional imaging by optical sectioning in the aberration-corrected scanning transmission electron microscope

Behan

Cosgriff

Kirkland

et al. 2009

Phil. Trans. R. Soc. A.

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The depth resolution for optical sectioning in the scanning transmission electron microscope is measured using the results of optical sectioning experiments of laterally extended objects. We show that the depth resolution depends on the numerical aperture of the objective lens as expected. We also find, however, that the depth resolution depends on the lateral extent of the object that is being imaged owing to a missing cone of information in the transfer function. We find that deconvolution methods generally have limited usefulness in this case, but that three-dimensional information can still be obtained with the aid of prior information for specific samples such as those consisting of supported nanoparticles. We go on to review how a confocal geometry may improve the depth resolution for extended objects. Finally, we present a review of recent work exploring the effect of dynamical diffraction in zone-axis-aligned crystals on the optical sectioning process.

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Section: (A) Three-dimensional Transfer and Resolution In Incoherent mentioning

confidence: 97%

Three-dimensional imaging by optical sectioning in the aberration-corrected scanning transmission electron microscope

Behan

Cosgriff

Kirkland

et al. 2009

Phil. Trans. R. Soc. A.

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“…Note however that the observed elongation along the rotation axis in diffractive tomography with sample rotation is less pronounced than that observed in standard transmission microscopy or in fluorescence microscopy along the optical axis [16,17]. This can be easily understood if one considers the so-called missing-cone in transmission or fluorescence microscopy [18]: while high frequencies are captured along lateral axes, no frequencies are measured along the optical axis, and even the maximum thickness of the captured frequency support along this direction is several times smaller than its lateral extension. Note that this frequency support has the same shape in diffractive tomography with variable illumination [1][2][3].…”

Section: Simulationsmentioning

confidence: 91%

Diffraction microtomography with sample rotation: influence of a missing apple core in the recorded frequency space

Vertu¹,

Delaunay²,

Yamada³

et al. 2009

Open Physics

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Abstract:Diffraction microtomography in coherent light is foreseen as a promising technique to image transparent living samples in three dimensions without staining. Contrary to conventional microscopy with incoherent light, which gives morphological information only, diffraction microtomography makes it possible to obtain the complex optical refractive index of the observed sample by mapping a three-dimensional support in the spatial frequency domain. The technique can be implemented in two configurations, namely, by varying the sample illumination with a fixed sample or by rotating the sample using a fixed illumination. In the literature, only the former method was described in detail. In this report, we precisely derive the three-dimensional frequency support that can be mapped by the sample rotation configuration. We found that, within the first-order Born approximation, the volume of the frequency domain that can be mapped exhibits a missing part, the shape of which resembles that of an apple core. The projection of the diffracted waves in the frequency space onto the set of sphere caps covered by the sample rotation does not allow for a complete mapping of the frequency along the axis of rotation due to the finite radius of the sphere caps. We present simulations of the effects of this missing information on the reconstruction of ideal objects. ; 42.30.-d; 42.40.-i; 42.90.+m PACS (2008): 42.

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“…The aim of all TDM set-ups is to increase this set of recorded frequencies in order to improve the lateral and/or longitudinal resolution. For tomographic diffractive microscopy with illumination rotation [11,12,14], one usually uses a condenser to control the incidence of the illumination, with same NA as the objective used for detection (other configurations have been described in [22]). For a full scanning of the condenser aperture, the OTF of the system takes the "doughnut" shape described by Fig.…”

Section: Tdm With Illumination Rotationmentioning

confidence: 99%

Improved and isotropic resolution in tomographic diffractive microscopy combining sample and illumination rotation

et al. 2011

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Abstract:Tomographic Diffractive Microscopy is a technique, which permits to image transparent living specimens in three dimensions without staining. It is commonly implemented in two configurations, by either rotating the sample illumination keeping the specimen fixed, or by rotating the sample using a fixed illumination. Under the first-order Born approximation, the volume of the frequency domain that can be mapped with the rotating illumination method has the shape of a "doughnut", which exhibits a so-called "missing cone" of non-captured frequencies, responsible for the strong resolution anisotropy characteristic of transmission microscopes. When rotating the sample, the resolution is almost isotropic, but the set of captured frequencies still exhibits a missing part, the shape of which resembles that of an apple core. Furthermore, its maximal extension is reduced compared to tomography with rotating illumination. We propose various configurations for tomographic diffractive microscopy, which combine both approaches, and aim at obtaining a high and isotropic resolution. We illustrate with simulations the expected imaging performances of these configurations. 42.30.-d, 42.40.-i, 42.90.+m PACS (2008):

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Three-dimensional imaging by a microscope

Cited by 365 publications

References 5 publications

Three-dimensional imaging by optical sectioning in the aberration-corrected scanning transmission electron microscope

Three-dimensional imaging by optical sectioning in the aberration-corrected scanning transmission electron microscope

Diffraction microtomography with sample rotation: influence of a missing apple core in the recorded frequency space

Improved and isotropic resolution in tomographic diffractive microscopy combining sample and illumination rotation

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