Three-dimensional identification of stem cells by computational holographic imaging

Abstract

We present an optical imaging system and mathematical algorithms for three-dimensional sensing and identification of stem cells. Data acquisition of stem cells is based on holographic microscopy in the Fresnel domain by illuminating the cells with a laser. In this technique, the holograms of stem cells are optically recorded with an image sensor array interfaced with a computer and three-dimensional images of the stem cells are reconstructed from the Gabor-filtered digital holograms. The Gabor wavelet transformation for feature extraction of the digital hologram is performed to improve the process of identification. The inverse Fresnel transformation of the Gabor-filtered digital hologram is performed to reconstruct the multi-scale three-dimensional images of the stem cells at different depths along the longitudinal direction. For recognition and classification of stem cells, a statistical approach using an empirical cumulative density function is introduced. The experiments indicate that the proposed system can be potentially useful for recognizing and classifying stem cells. To the best of our knowledge, this is the first report on using three-dimensional holographic microscopy for automated identification of stem cells.

Digital Holography and Cell Studies

“…Stem cells are usually studied with flow cytometry and fluorescence microscopy. In 2007, a DHM study of sunflower and corn stem cells was performed by Moon and Javidi (Moon & Javidi, 2007). DHM was used for automated plant stem cell monitoring, sensing and identification.…”

“…Digital holography has been used to study a wide range of cells, e.g. protozoa, bacteria and plant cells as well as several types of mammalian cells such as nerve cells and tumor cells (Emery et al, 2007;Kemper et al, 2006;Moon and Javidi 2007). It has also been applied for studies of cell proliferation, cell movement and cell morphology Yu et al, 2009).…”

Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy

“…This unique feature has been determinant to find applications of DHM in living cell screening, [1][2][3][4] particle tracking, [5][6][7][8][9][10][11] and MEMS evaluation, [12][13][14] among many others. [15][16][17][18][19][20][21][22][23][24] The ability of performing QPI has been extended to other microscopy methods like spatial light interference microscopy or spatial light interference tomography; 25,26 hence similar applications have been reported by the use of these imaging approaches. In DHM, the fringe pattern recorded after the interference between a reference wave and the image of the wave diffracted by the microscopic sample allows the retrieval of the phase changes introduced by the sample.…”

Cells and Holograms – Holograms and Digital Holographic Microscopy as a Tool to Study the Morphology of Living Cells

“…protozoa, bacteria and plant cells, mammalian cells such as nerve cells, stem cells, various tumor cells, bacterial-cell interactions, red blood cells and sperm cells [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18].…”