Three-dimensional hydrodynamic focusing with a single sheath flow in a single-layer microfluidic device

Lee, Myung Gwon; Choi, Sungyoung; Park, Je‐Kyun

doi:10.1039/b910712f

Cited by 109 publications

(85 citation statements)

References 22 publications

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“…They developed a microfluidic flow cytometer in which the sample fluid was thus confined to a small volume ͑20ϫ 34ϫ 30 m 3 ͒ to reduce variations in detection signal intensities. 26 Similarly, Lee et al 27 used a contraction-expansion array microchannel ͓Fig. 3͑b͔͒ to achieve threedimensional flow focusing in a single-layer device.…”

Section: A Hydrodynamic Focusingmentioning

confidence: 99%

Review Article: Recent advancements in optofluidic flow cytometer

Cho¹,

Godin²,

Chen³

et al. 2010

Biomicrofluidics

139

105

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There is an increasing need to develop optofluidic flow cytometers. Optofluidics, where optics and microfluidics work together to create novel functionalities on a small chip, holds great promise for lab-on-a-chip flow cytometry. The development of a low-cost, compact, handheld flow cytometer and microfluorescence-activated cell sorter system could have a significant impact on the field of point-of-care diagnostics, improving health care in, for example, underserved areas of Africa and Asia, that struggle with epidemics such as HIV/AIDS. In this paper, we review recent advancements in microfluidics, on-chip optics, novel detection architectures, and integrated sorting mechanisms.

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Section: A Hydrodynamic Focusingmentioning

confidence: 99%

Review Article: Recent advancements in optofluidic flow cytometer

Cho¹,

Godin²,

Chen³

et al. 2010

Biomicrofluidics

139

105

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“…The acceleration of fluid in the contraction area can produce a Dean-like counter-rotating flow (vortex). Recently, this secondary flow effect was already explored to applications of particle focusing and separation [37,45,48]. The secondary flow drag F D , which is due to the difference between particle velocity and fluid velocity in cross-sectional plane, can be calculated by stokes drag law:…”

Section: Secondary Flow In the Cross-sectional Planementioning

confidence: 99%

“…Furthermore, bio-particles were circulated in horizontal vortex to study the effects of centrifugal force and shear stress on their morphology [41][42][43]. The secondary flow in cross-sectional plane has been applied to focus [44][45] and separate particles [36][37]. Park et al [44] presented a multi-orifice microfluidic channel to focus microparticles by the combination of inertial migration and secondary flow in cross-sectional plane.…”

Section: Introductionmentioning

confidence: 99%

“…In their device, the cavity was rectangular and symmetrically patterned on two sides of a straight channel. Inspired from the idea that asymmetry can improve the sorting efficiency in spiral channel [11], Lee et al [36][37]45] developed a microfluidic device with asymmetrically patterned rectangular cavity arrays to achieve a three dimensional focusing and extraction of blood cells from blood plasma with a 62.2% yield and 1.2 ml h -1 throughput. However, a sheath flow was needed while their device operating, which brought potential drawbacks of dilution and contamination on bio-particles sample, as well as complication of the whole microfluidic system.…”

Section: Introductionmentioning

confidence: 99%

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Inertial focusing in a straight channel with asymmetrical expansion–contraction cavity arrays using two secondary flows

Zhang

et al. 2013

J. Micromech. Microeng.

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G. (2013). Inertial focusing in a straight channel with asymmetrical expansion-contraction cavity arrays using two secondary flows. Journal of Micromechanics and Microengineering, 23 (8), 1-13. Inertial focusing in a straight channel with asymmetrical expansioncontraction cavity arrays using two secondary flows AbstractThe focusing of particles has a variety of applications in industry and biomedicine, including wastewater purification, fermentation filtration, and pathogen detection in flow cytometry, etc. In this paper a novel inertial microfluidic device using two secondary flows to focus particles is presented. The geometry of the proposed microfluidic channel is a simple straight channel with asymmetrically patterned triangular expansion-contraction cavity arrays. Three different focusing patterns were observed under different flow conditions: (1) a single focusing streak on the cavity side; (2) double focusing streaks on both sides; (3) half of the particles were focused on the opposite side of the cavity, while the other particles were trapped by a horizontal vortex in the cavity. The focusing performance was studied comprehensively up to flow rates of 700 μl min−1. The focusing mechanism was investigated by analysing the balance of forces between the inertial lift forces and secondary flow drag in the cross section. The influence of particle size and cavity geometry on the focusing performance was also studied. The experimental results showed that more precise focusing could be obtained with large particles, some of which even showed a single-particle focusing streak in the horizontal plane. Meanwhile, the focusing patterns and their working conditions could be adjusted by the geometry of the cavity. This novel inertial microfluidic device could offer a continuous, sheathless, and high-throughput performance, which can be potentially applied to high-speed flow cytometry or the extraction of blood cells. (1) single focusing streak on cavity side; (2) double focusing streaks on both sides; (3) half of particles focused on the opposite side of cavity, and other particles trapped by horizontal vortex in cavity. The focusing performance was studied comprehensively up to the flow rates of 700 µl min -1 . The focusing mechanism was investigated by analyzing force balance between inertial lift forces and secondary flow drag in the cross section.The influence of particle size and cavity geometry on the focusing performance was also studied. Experimental results showed that a more pronounced focusing performance can be obtained with large particles, which even showed a single-particle focusing streak in horizontal plane. Meanwhile, focusing patterns and their working conditions were adjustable by the geometry of cavity. The novel inertial microfluidic device was capable of offering a continuous, sheathless, and high-throughput performance, which can be potentially applied to highspeed flow cytometry or extraction of blood cells.

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“…10,12,[40][41][42][43][44][45][46][47][48][49][50][51][52][53] In these approaches, the key is to develop microfluidic structures to focus particles/cells three-dimensionally. [54][55][56][57][58][59][60][61][62][63][64][65] To this end, we developed a three-dimensional (3D) hydrodynamic focusing technique called "microfluidic drifting." 10,13 By utilizing the Dean flow 65,66 in a curved microfluidic channel, "microfluidic drifting" enables 3D hydrodynamic focusing in a single-layer planar microfluidic device that can be readily fabricated via standard soft-lithography.…”

Section: Introductionmentioning

confidence: 99%

An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing

et al. 2012

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In this work, we demonstrate an integrated, single-layer, miniature flow cytometry device that is capable of multi-parametric particle analysis. The device integrates both particle focusing and detection components on-chip, including a "microfluidic drifting" based three-dimensional (3D) hydrodynamic focusing component and a series of optical fibers integrated into the microfluidic architecture to facilitate onchip detection. With this design, multiple optical signals (i.e., forward scatter, side scatter, and fluorescence) from individual particles can be simultaneously detected. Experimental results indicate that the performance of our flow cytometry chip is comparable to its bulky, expensive desktop counterpart. The integration of on-chip 3D particle focusing with on-chip multi-parametric optical detection in a singlelayer, mass-producible microfluidic device presents a major step towards low-cost flow cytometry chips for point-of-care clinical diagnostics. V C 2012 American Institute of Physics. [http://dx

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Three-dimensional hydrodynamic focusing with a single sheath flow in a single-layer microfluidic device

Cited by 109 publications

References 22 publications

Review Article: Recent advancements in optofluidic flow cytometer

Review Article: Recent advancements in optofluidic flow cytometer

Inertial focusing in a straight channel with asymmetrical expansion–contraction cavity arrays using two secondary flows

An integrated, multiparametric flow cytometry chip using “microfluidic drifting” based three-dimensional hydrodynamic focusing

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