Three-dimensional homonuclear TOCSY-NOESY of nucleic acids. Possibilities for improved assignments

The Hairpin Structure of a Topoisomerase II Site DNA Strand Analyzed by Combined NMR and Energy Minimization Methods

“…Once every H1', H2' and H2" proton was assigned, the analysis of the H1' (v3 dimension) planes allowed the identification of the H3' and H4' protons through the detection of information transfers. However, the limited resolution and degeneracy of the H5' and H5" resonances limited their stereospecific assignment to only the T10 and C11 residues (analysis of H3' (v3 dimension) planes: Mooren et al, 1991).…”

The detailed structure of tandem G·A mismatched base-pair motifs in RNA duplexes is context dependent

“…The 600 MHz 3D TOCSY-NOESY (Mooren et al, 1991;Wijmenga et al, 1994) of the UGAA sample was recorded and processed as described previously . A TOCSY mixing time of 78 ms was used to obtain suf®cient coherence transfer.…”

The Transition from a Neutral-pH Double Helix to a Low-pH Triple Helix Induces a Conformational Switch in the CCCG Tetraloop Closing a Watson-Crick Stem

“…Subsequently, the non-exchangeable base and sugar ring protons of the molecule were assigned by analysis of spectra recorded in 2 H 2 O. Residue specific and sequential resonance assignments were carried out by standard procedures, using NOESY, P.E.COSY and TOCSY spectra (Wijmenga et al, 1993), complemented by data obtained from a 1 H-31 P hetero-correlation spectrum (Pardi et al, 1983) and a selective version of a 3D-TOCSY-NOESY spectrum (Mooren et al, 1991a;Wijmenga et al, 1994). A comparison of J-coupling values and relative NOE intensities resulted in the stereospecific assignments for the H5' and H5" resonances of C8, C9, and G10.…”