Three-dimensional fluorescence imaging through dynamic scattering media by motionless optical scanning holography

Abstract

Three-dimensional (3D) fluorescence imaging is an essential technique in the biomedical field. In particular, 3D fluorescence imaging through dynamic scattering media is a crucial task for the minimally invasive observation of labeled cells. In this study, this task was accomplished via motionless optical scanning holography, proposed as a single-pixel 3D imaging technique. The proposed method does not require additional computational processing or optical components when the detected intensities do not considerably fluctuate irrespective of the presence of dynamic scattering media. The results of a proof-of-principle experiment indicated that the proposed method can help in computationally refocusing fluorescent objects that are placed at different positions behind dynamic scattering media.

Roadmap of incoherent digital holography

“…Besides OSH and FINCH, many research groups reported holographic fluorescence microscopy systems with different types of interferometers: a Michelson interferometer [45,186,187], a single-path off-axis self-interference system [188,189], and a single-path self-interference system using a grating for quadriwave lateral shearing interferometry [190]. As other research achievements in IDHM, 3D fluorescence imaging through static or moving scattering media was performed with an OSH system [191], which exploits the nature of single-pixel DH. IDHM was also combined with lattice light-sheet microscopy for depth-resolved holographic 3D imaging [192] and Raman scattering microscopy for 3D imaging of surface-enhanced Raman scattering light [193].…”

Review of Incoherent Digital Holography: Applications to Multidimensional Incoherent Digital Holographic Microscopy and Palm-Sized Digital Holographic Recorder—Holosensor

“…Any light source including self-luminous light and natural light can be recorded as a hologram. Various techniques such as 3D microscopy for self-luminous light (Schilling et al, 1997;Rosen and Brooker, 2008;Jang et al, 2015;Yanagawa et al, 2015;Quan et al, 2017;Liebel et al, 2020a;Liebel et al, 2020b;Kumar et al, 2020;Marar and Kner, 2020;Potcoava et al, 2021), 3D imaging with a single image sensor (Kim, 2013;Clark and Kim, 2015;Jang et al, 2015;Vijayakumar et al, 2016;Vijayakumar and Rosen, 2017;Nobukawa et al, 2018;Choi et al, 2019;Nobukawa et al, 2019;Wu et al, 2020;Tahara et al, 2021d;Wu et al, 2021;Yoneda et al, 2021;Tahara et al, 2022), 3D thermal measurement (Imbe, 2019), and spectroscopic 3D imaging (Yoshimori, 2001;Teeranutranont and Yoshimori, 2013;Naik et al, 2014;Kalenkov et al, 2019) have been proposed to date. Research on applications of IDH is ongoing and a challenging theme.…”

Spatially divided two-step phase-shifting method for computational optical scanning holography

“…For these reasons, OSH is an incoherent digital holography with the advantages of SPI. OSH has been applied to various applications such as multicolor imaging [16], stereo-lightning reconstruction [17], imaging through scattering media [18], fluorescence imaging [19], polarization imaging [20], and so on. Interestingly, by using a pinhole just before the detector at a Fourier plane of the object, holograms detected with a laser, coherent holograms, can be obtained, which means OSH can also be applied to quantitative phase imaging [21].…”