Three-dimensional environments preserve extracellular matrix compartments of ovarian follicles and increase FSH-dependent growth

Gomes, José Eugênio P. Lima; Correia, Sónia C.; Gouveia-Oliveira, A; Cidadão, António; Plancha, Carlos E.

doi:10.1002/(sici)1098-2795(199910)54:2<163::aid-mrd8>3.0.co;2-4

Cited by 47 publications

(29 citation statements)

References 38 publications

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“…This improved culture environment allowed investigation of the important physiological roles of insulin-related factors and gonadotropins on the growth, development, and differentiation of preantral follicles to the antral stage. Gomes et al [23] revealed that the flat/adhesive cultures of mouse preantral follicles led to distortion of follicular morphology and frequent follicle disruption, but three-dimensional collagen gel cultures were able to support normal follicular structure and higher growth of preantral follicles in response to FSH. In our study, a three-dimensional collagen gel culture system with the use of a serum-free medium was able to maintain healthy follicles for the long term.…”

Section: Discussionmentioning

confidence: 99%

Growth, Antrum Formation, and Estradiol Production of Bovine Preantral Follicles Cultured in a Serum-Free Medium1

Itoh

Kacchi

Abé

et al. 2002

Biology of Reproduction

114

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The objective of this study was to identify factors that would allow the establishment of a serum-free culture system that could support follicular and oocyte growth, antrum formation, and estradiol-17beta (E(2)) production in preantral follicles of bovine ovaries. Large preantral follicles (145-170 micro m in diameter) were microsurgically dissected from ovaries, embedded in 0.15% type I collagen gels, and maintained in a serum-free medium for up to 13 days at 38.5 degrees C in 5% CO(2) in air. This culture environment allowed most preantral follicles to maintain a three-dimensional structure with the presence of a thecal layer and basement membrane surrounding the granulosa cells throughout the entire culture period. The effects of insulin, insulin-like growth factor (IGF)-I, IGF-II, FSH, and LH on preantral follicle growth were investigated in serum-free medium. Follicular diameters were significantly larger in the presence of insulin, IGF-I, IGF-II, or FSH after 13 days in culture. Oocyte diameters were also significantly larger in the presence of all hormones tested. The single addition of insulin, IGF-I, or FSH induced antrum formation between Days 7 and 13 of culture. Insulin progressively induced E(2) secretion by follicles after antrum formation, but IGF-I and FSH had no apparent effect. FSH and LH caused an increase in oocyte diameter in the presence of insulin. The addition of three hormones (insulin, FSH, and LH) initiated antrum formation and E(2) production earlier than insulin-containing medium alone. Furthermore, maximal E(2) secretion was maintained steadily between 7 and 13 days in this culture condition. From these results, we have demonstrated that insulin, FSH, and LH play substantial roles in the growth and development of bovine large preantral follicles in a serum-free medium.

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Section: Discussionmentioning

confidence: 99%

Growth, Antrum Formation, and Estradiol Production of Bovine Preantral Follicles Cultured in a Serum-Free Medium1

Itoh

Kacchi

Abé

et al. 2002

Biology of Reproduction

114

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“…Normal granulosa cell morphology in vivo is round, yet granulosa cells seeded onto uncoated tissue culture plastic resemble fibroblasts, flattened in a monolayer and spread with little interaction between neighboring cells. [13][14][15][16][17][18][19][20] When plated on type I collagen gels, isolated basal lamina, or Matrigel, however, granulosa cells retain their spherical, epithelioid shape. 13,16,17,21,22 Granulosa cell morphology is also determined by its cytoskeleton, the composition of which is influenced by the surrounding ECM.…”

Section: Regulation Of Ovarian Cell Morphologymentioning

confidence: 99%

The Role of the Extracellular Matrix in Ovarian Follicle Development

Woodruff

Shea²

2007

Reprod. Sci.

162

117

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Regulation of ovarian follicle development depends on endocrine- and paracrine-acting hormones, the 3-dimensional architecture of the follicle, and the physical rigidity of the surrounding tissue. These 3 forces are integrated throughout the life cycle of the follicle to ensure appropriate hormone secretion, differentiation of the somatic cells, and maturation of the oocyte. The process of in-follicle maturation provides a new tool for understanding ovarian follicle development under the influence of these factors.

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“…Two methods of preparation of oocytes and follicular cells have been reported: enzymatic isolation of oocyte-granulosa cell complexes from preantral follicles (Eppig & Schroeder, 1989;Torrance et al, 1989) and mechanical dissection of preantral follicles (Nayudu & Osborn, 1992;Boland et al, 1993;. The mechanical dissection isolates intact follicles and their development in vitro provides the only model allowing all in vitro developing cells to retain their in vivo relationships and to proceed through sequential developmental stages (Gomes et al, 1999). Different culture systems have been used: organotypic culture where structural integrity is maintained and monolayer culture with adherence of cells to the support.…”

Section: Introductionmentioning

confidence: 99%

Comparison of three in vitro culture systems for maturation of early preantral mouse ovarian follicles

Mousset-Siméon

Jouannet

Cointre

et al. 2005

Zygote

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The aim of this study was to compare three different culture systems for in vitro follicular growth and oocyte maturation in ovarian follicles of mice in order to assess the technique with the optimal growth and improved rate of meiotic maturation. The three systems tested were culture under oil, on a hydrophobic membrane and on agar respectively. Early preantral follicles were cultured for 12 days in α-MEM GlutaMAX medium. Follicular growth, oocyte meiotic maturation, oocyte extrusion, atresia and estradiol production were analysed. Follicular development showed two phases in the three systems, with slow growth before day 5 and subsequent acceleration. The percentage of follicles transferred into oocyte maturation medium was significantly higher after culture under oil. The proportion of oocytes that achieved nuclear maturation (metaphase II) was higher when follicles were cultured under oil or on a hydrophobic membrane than on agar. Our results support the use of culture under oil for in vitro follicular growth from the early preantral stage in order to obtain metaphase II oocytes. Fertilization ability of these oocytes and the capacity to obtain healthy mice in a reproducible manner warrants further investigation.

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Three-dimensional environments preserve extracellular matrix compartments of ovarian follicles and increase FSH-dependent growth

Cited by 47 publications

References 38 publications

Growth, Antrum Formation, and Estradiol Production of Bovine Preantral Follicles Cultured in a Serum-Free Medium1

Growth, Antrum Formation, and Estradiol Production of Bovine Preantral Follicles Cultured in a Serum-Free Medium1

The Role of the Extracellular Matrix in Ovarian Follicle Development

Comparison of three in vitro culture systems for maturation of early preantral mouse ovarian follicles

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