Three‐dimensional direct cell bioprinting for tissue engineering

This scientometric analysis of 393 original papers published from January 2000 to June 2019 describes the development and use of bioinks for 3D bioprinting. The main trends for bioink applications and the primary considerations guiding the selection and design of current bioink components (i.e., cell types, hydrogels, and additives) were reviewed. The cost, availability, practicality, and basic biological considerations (e.g., cytocompatibility and cell attachment) are the most popular parameters guiding bioink use and development. Today, extrusion bioprinting is the most widely used bioprinting technique. The most reported use of bioinks is the generic characterization of bioink formulations or bioprinting technologies (32%), followed by cartilage bioprinting applications (16%). Similarly, the cell-type choice is mostly generic, as cells are typically used as models to assess bioink formulations or new bioprinting methodologies rather than to fabricate specific tissues. The cell-binding motif arginine-glycine-aspartate is the most common bioink additive. Many articles reported the development of advanced functional bioinks for specific biomedical applications; however, most bioinks remain the basic compositions that meet the simple criteria: Manufacturability and essential biological performance. Alginate and gelatin methacryloyl are the most popular hydrogels that meet these criteria. Our analysis suggests that present-day bioinks still represent a stage of emergence of bioprinting technology.

show abstract

“…Bioinks may consist solely of cells[ 94 , 145 , 146 ]. However, 13 of the analyzed documents used cell aggregates or cell spheroids as bioinks.…”

Section: Bioinks For Different Bioprinting Technologiesmentioning

confidence: 99%

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Pedroza-González¹,

Rodríguez-Salvador²,

Pérez-Benítez³

et al. 2021

ijb

View full text Add to dashboard Cite

show abstract

“… Schematic representation of a three‐dimensional printing device with (a) solution electrospinning, (b) melt electrospinning, and (c) fused deposition modeling . [Color figure can be viewed at http://wileyonlinelibrary.com]…”

Section: Basic Principles Of Traditional Electrospinning Near Field mentioning

confidence: 99%

“…The setup uses a heating coil along with a temperature control in order to provide enough heat to melt the polymer. The raw material commonly used in melt electrospinning writing is granular material, while the filamentous thermoplastic material is usually used to the fused deposition modeling technology (3D printing), as shown in Figure (c). Melt electrospinning writing has a high viscosity and low electrical conductivity requiring the needle–collector distance to be a wide range .…”

Section: Basic Principles Of Traditional Electrospinning Near Field mentioning

confidence: 99%

Recent studies on electrospinning preparation of patterned, core–shell, and aligned scaffolds

Liu

Mukherjee

Liu

et al. 2018

J of Applied Polymer Sci

View full text Add to dashboard Cite

Electrospinning is one of the most important ways to prepare continuous, high porosity, large specific surface area, and uniform diameter micro-and nanoscale fibers. So, it has been widely used in the preparation of micro/nano-sized polymer scaffolds for tissue engineering in recent years. In addition to the versatility in material selection and the processing variables, electrospinning also provides a lot of methods to regulate fiber structure and scaffolds morphology. For example, the near-field electrospinning can provide a method to solve the problem of uncontrollable fiber path; the melt electrospinning eliminates the risk of solvent residue in the construct; the addition of different auxiliary electrodes can make the fiber patterned. This review introduces the underlying principle and characteristics of above electrospinning applied in biomedicine. Herein, we highlight a comprehensive understanding of the technical aspect of this technology for versatile fibers with patterned, core-shell and aligned morphology.

show abstract

“…Ozler et al reported multicellular aggregates used for direct cell 3D printing with smooth muscle, endothelial and fibroblast cells. They demonstrated the ability of these constructs to fuse among one another with high cell viability throughout the study [ 40 ]. Even though they have shown good gelation properties, chemical modifications or blending is required for maintaining the 3D printed structure and to enhance more cellular functions.…”

Section: Introductionmentioning

confidence: 99%

Recent trends in bioinks for 3D printing

2018

View full text Add to dashboard Cite

BackgroundThe worldwide demand for the organ replacement or tissue regeneration is increasing steadily. The advancements in tissue engineering and regenerative medicine have made it possible to regenerate such damaged organs or tissues into functional organ or tissue with the help of 3D bioprinting. The main component of the 3D bioprinting is the bioink, which is crucial for the development of functional organs or tissue structures. The bioinks used in 3D printing technology require so many properties which are vital and need to be considered during the selection. Combination of different methods and enhancements in properties are required to develop more successful bioinks for the 3D printing of organs or tissue structures.Main bodyThis review consists of the recent state-of-art of polymer-based bioinks used in 3D printing for applications in tissue engineering and regenerative medicine. The subsection projects the basic requirements for the selection of successful bioinks for 3D printing and developing 3D tissues or organ structures using combinations of bioinks such as cells, biomedical polymers and biosignals. Different bioink materials and their properties related to the biocompatibility, printability, mechanical properties, which are recently reported for 3D printing are discussed in detail.ConclusionMany bioinks formulations have been reported from cell-biomaterials based bioinks to cell-based bioinks such as cell aggregates and tissue spheroids for tissue engineering and regenerative medicine applications. Interestingly, more tunable bioinks, which are biocompatible for live cells, printable and mechanically stable after printing are emerging with the help of functional polymeric biomaterials, their modifications and blending of cells and hydrogels. These approaches show the immense potential of these bioinks to produce more complex tissue/organ structures using 3D bioprinting in the future.

show abstract

Three‐dimensional direct cell bioprinting for tissue engineering

Cited by 26 publications

References 47 publications

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress

Recent studies on electrospinning preparation of patterned, core–shell, and aligned scaffolds

Recent trends in bioinks for 3D printing

Contact Info

Product

Resources

About