Three dimensional digital holographic profiling of micro-fibers

Abstract

Abstract:A method to measure the size, orientation, and location of opaque micro-fibers using digital holography is presented. The method involves the recording of a digital hologram followed by reconstruction at different depths. A novel combination of automated image analysis and statistical techniques, applied on the intensity of reconstructed digital holograms is used to accurately determine the characteristics of the microfibers. The performance of the proposed method is verified with a single fiber of known length and orientation. The potential of the method for measurement of fiber length is further demonstrated through its application to a suspension of fibers in a liquid medium.

Localization of Microfibers within Volumes Using Digital Holographic Video

“…The method proposed here takes into account the orientation of the fiber and hence measures its length correctly. As a result, it can be used for accurate measurement of fibers in suspension [6].…”

Principles and techniques of digital holographic microscopy

“…The method is more effective the smaller the object is, so that the reference is not excessively disturbed. Because of this constraint and because of the simplicity of the optical setup, Gabor holography is particularly useful for particle image analysis [109][110][111] as well as for thin fibers [112]. Digital Gabor holography (DGH) can provide new capabilities for wider applications of this simple configuration [113][114][115].…”

“…Dubois and Grosfils introduced dark-field digital holography for studying nanoparticles smaller than the optical resolution [189]. DGH is also used for measuring the 3-D position and orientation of a microfiber segment [112]. Biomedical particulate imaging applications of DGH have been noted earlier.…”

Segmentation and visualization of digital in-line holographic microscopy of three-dimensional scenes using reconstructed intensity images

“…biological samples 5 or small opaque objects sparsly distributed in the field of view. 13 This limits applicability of DIHM as described in chapter 9 of.…”

“…[5][6][7][8][9][10][11][12][13] Previous 2D segmentation of digital holographic microscopy intensity reconstructions used active contours.…”