Three‐dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain

Aggarwal, Manisha; Mori, Susumu; Shimogori, Tomomi; Blackshaw, Seth; Zhang, Jiangyang

doi:10.1002/mrm.22426

Cited by 84 publications

(148 citation statements)

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“…A number of authors (24)(25)(26)(27)38) have elegantly demonstrated the value of MRI diffusionbased tractography in understanding the microstructural mouse brain organization and its intricate connectional anatomy. These ex vivo investigations were performed using ultra- (26,27) or high-field magnets (24,25,38), and revealed brain anatomical details with a resolution of reconstructed fiber density maps reaching 20 μm 3 (27). By using a unique combination of global tractography and fiber-mapping methods, we tailored our hrFM to reach the optical microscopy level, identifying with high definition connectional networks across the whole living mouse brain.…”

Section: Discussionmentioning

confidence: 99%

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Mapping remodeling of thalamocortical projections in the living reeler mouse brain by diffusion tractography

Harsan

Dávid

Reisert

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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A major challenge in neuroscience is to accurately decipher in vivo the entire brain circuitry (connectome) at a microscopic level. Currently, the only methodology providing a global noninvasive window into structural brain connectivity is diffusion tractography. The extent to which the reconstructed pathways reflect realistic neuronal networks depends, however, on data acquisition and postprocessing factors. Through a unique combination of approaches, we designed and evaluated herein a framework for reliable fiber tracking and mapping of the living mouse brain connectome. One important wiring scheme, connecting gray matter regions and passing fibercrossing areas, was closely examined: the lemniscal thalamocortical (TC) pathway. We quantitatively validated the TC projections inferred from in vivo tractography with correlative histological axonal tracing in the same wild-type and reeler mutant mice. We demonstrated noninvasively that changes in patterning of the cortical sheet, such as highly disorganized cortical lamination in reeler, led to spectacular compensatory remodeling of the TC pathway.fiber tracking validation | brain developmental plasticity M apping the brain's neural architecture and its connectivity fingerprints is essential in experimental neuroscience and neurology because connectivity patterns constrain or even define functional neuronal networks (1). Methods for tracing connections in the animal brain have a long history and evolved from silver impregnation (2) of degenerating fibers to the ex vivo visualization of axonally transported tracers injected in different brain nuclei (3), and finally to high-resolution technologies using viral and genetic tracers (4). Exploiting the active transport mechanisms along the axons, the histological tract-tracing methods are of extreme value in addressing neuroanatomical questions in experimental animals, especially when associated with electrophysiological and behavioral observations (5). One challenging and intensively investigated issue is the dynamic interplay between the formation of cortical connectivity and cortical patterning (6). Because of the regional and laminar specificity of axonal targeting, the thalamocortical projection (TCP) system is a primary research focus (7-9); it offers the possibility of examining the way in which cortical patterning and thalamocortical (TC) wiring shape and constrain each other. Axonal tracer studies have produced a large body of evidence concerning TC architecture in experimental animals, suggesting that changes in patterning of the cortical sheet might result in alterations of TC connectivity (10-12). However, axonal tract-tracing is not suitable for monitoring plastic connectivity changes over time in the same individual. Histological visualization of the transported substance is highly invasive and requires the sacrifice of the animal; it allows the identification of only a limited number of pathways terminating in, or originating from, the injection site in a single animal.One of the most exciting recent deve...

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Section: Discussionmentioning

confidence: 99%

“…Many cellular structures or fiber pathways of biological interest are small in comparison with the scale of the imaging voxel. Increased signal-to-noise ratio (SNR) and further improvement of the spatial resolution could, however, be achieved with high (24,25) and ultrahigh-field scanners (26), or at the expense of very long acquisition times.…”

mentioning

confidence: 99%

Mapping remodeling of thalamocortical projections in the living reeler mouse brain by diffusion tractography

Harsan

Dávid

Reisert

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…Diffusion tensor data were acquired using the diffusionweighted three-dimensional (3D) GRASE sequence with the following parameters: TE = 33 ms; TR = 800 ms; bandwidth = 100 kHz; FOV = 17.0 · 14.0 · 25.8 mm; matrix size = 128 · 96 · 192 (zeropadded to 256 · 192 · 384); and number of averages = 4. 22 For DTI, six diffusion-weighted images (b-value, 1700 s/mm 2 ) and two nondiffusion-weighted images were acquired with d = 3 ms and D = 15 ms. Diffusion tensors were calculated using a log-linear fitting method using DTIStudio (http://www.mristudio.org), and fractional anisotropy (FA) maps were calculated from the tensor data.…”

Section: Diffusion Tensor Imagingmentioning

confidence: 99%

Evidence for Impaired Plasticity after Traumatic Brain Injury in the Developing Brain

Yang

Glover

et al. 2014

Journal of Neurotrauma

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The robustness of plasticity mechanisms during brain development is essential for synaptic formation and has a beneficial outcome after sensory deprivation. However, the role of plasticity in recovery after acute brain injury in children has not been well defined. Traumatic brain injury (TBI) is the leading cause of death and disability among children, and long-term disability from pediatric TBI can be particularly devastating. We investigated the altered cortical plasticity 2-3 weeks after injury in a pediatric rat model of TBI. Significant decreases in neurophysiological responses across the depth of the noninjured, primary somatosensory cortex (S1) in TBI rats, compared to age-matched controls, were detected with electrophysiological measurements of multi-unit activity (86.4% decrease), local field potential (75.3% decrease), and functional magnetic resonance imaging (77.6% decrease). Because the corpus callosum is a clinically important white matter tract that was shown to be consistently involved in post-traumatic axonal injury, we investigated its anatomical and functional characteristics after TBI. Indeed, corpus callosum abnormalities in TBI rats were detected with diffusion tensor imaging (9.3% decrease in fractional anisotropy) and histopathological analysis (14% myelination volume decreases). Whole-cell patch clamp recordings further revealed that TBI results in significant decreases in spontaneous firing rate (57% decrease) and the potential to induce long-term potentiation in neurons located in layer V of the noninjured S1 by stimulation of the corpus callosum (82% decrease). The results suggest that post-TBI plasticity can translate into inappropriate neuronal connections and dramatic changes in the function of neuronal networks.

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“…DWI is inherently sensitive to motion to measure microscopic water displacement; and therefore, voluntary and involuntary movement can severely affect image quality [129,131,154]. The use of respiratory gating and the navigator sequence has been developed to reduce these artefacts [170].…”

Section: Limitations Of Dwi Studies In Animal Modelsmentioning

confidence: 99%

Current Developments In Mri For Assessing Rodent Models of Multiple Sclerosis

et al. 2014

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MRI is a key radiological imaging technique that plays an important role in the diagnosis and characterization of heterogeneous multiple sclerosis (MS) lesions. Various MRI methodologies such as conventional T 1/T 2 contrast, contrast agent enhancement, diffusion-weighted imaging, magnetization transfer imaging and susceptibility weighted imaging have been developed to determine the severity of MS pathology, including demyelination/remyelination and brain connectivity impairment from axonal loss. The broad spectrum of MS pathology manifests in diverse patient MRI presentations and affects the accuracy of patient diagnosis. To study specific pathological aspects of the disease, rodent models such as experimental autoimmune encephalomyelitis, virus-induced and toxin-induced demyelination have been developed. This review aims to present key developments in MRI methodology for better characterization of rodent models of MS.

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Three‐dimensional diffusion tensor microimaging for anatomical characterization of the mouse brain

Cited by 84 publications

References 46 publications

Mapping remodeling of thalamocortical projections in the living reeler mouse brain by diffusion tractography

Mapping remodeling of thalamocortical projections in the living reeler mouse brain by diffusion tractography

Evidence for Impaired Plasticity after Traumatic Brain Injury in the Developing Brain

Current Developments In Mri For Assessing Rodent Models of Multiple Sclerosis

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