Three-dimensional deconvolution microfluidic microscopy using a tilted channel

Abstract

Abstract. We have developed a microfluidic device that enables the computation of three-dimensional (3-D) images of flowing samples. Using a microfluidic channel that is tilted along the optical axis, we record several progressively defocused images of the flowing sample as it passes across the focal plane. The resulting focal stack is then deconvolved to generate 3-D images. Experimental results on flowing yeast cells reveal both volume and surface profile information. The microfluidic channel eliminates the need for a precise translation stage to control defocusing and enables high sample throughput in an insulated, nontoxic, liquid environment. The experimental device can be implemented in all existing microscopes as a modified slide stage and is ideally suited for 3-D profiling in flow cytometers. © 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)

Microarray Expression Profiling and Raman Spectroscopy Reveal Anti-Fatty Liver Action of Berberine in a Diet-Induced Larval Zebrafish Model

“…Here, we introduced a method for quantitative analysis and 3D imaging, which use deconvolution algorithms, rapidly and precisely reconstructing 3D objects, allowing a better visual understanding of the image data and a more accurate quantitative assessment of the 3D object (Pegard and Fleischer, 2013;Chakrova et al, 2016). Compared with the control group, the HCD feeding significantly induced the hepatic ROS levels increase, which was inhibited by BBR (5 mM).…”

Automated cell viability assessment using a microfluidics based portable imaging flow analyzer

“…These imaging systems are, in general, referred to as Imaging Flow Cytometry (IFC) systems. [18][19][20][21][22][23][24][25] While IFC systems offer the advantages of both microscopy (acquisition of morphological features from images and high spatial resolution) and flow cytometry (automation, high sensitivity, and high-throughput), 26 one of the major limitation is the requirement of using high speed cameras. Most of the reported IFC systems in the literature employed cameras with frame rates of few to several thousand frames per second.…”

Framework for morphometric classification of cells in imaging flow cytometry

“…Ethan et al, have demonstrated the use of micro-optics and microfluidics to simultaneously image multiple field-of-views, in order to achieve a high-throughput of about 20,000 beads per second (Gorthi et al, 2011). Microfluidic IFC has also been extended to capture 3D morphology of cells (Gorthi & Schonbrun, 2012;Fleischer & Pgard 2013;Wu et al, 2013;Sung et al, 2014). In microfluidics-based IFC, the cells in suspension are allowed to flow across the microfluidics channel and the video of the flow stream is captured using a high-speed microscopy system.…”