Three‐dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion

Iwai, Shinichi; Kishimoto, Satoko; Amano, Yuki; Nishiguchi, Akihiro; Matsusaki, Michiya; Takeshita, Akinori; Akashi, Mitsuru

doi:10.1002/jbm.a.36319

Cited by 9 publications

(5 citation statements)

References 30 publications

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“…The development of new biomaterials is occurring rapidly. This includes composites (Douglas et al, ), polymers (Gadomska‐Gajadhur et al, ), hydrogels (Jeznach et al, ; Niemczyk et al, ), as well as the design of new 3D architectures (Dulnik et al, ; Iwai et al, ; Kruk et al, ), or surface functionalization techniques (Choińska et al, ). All these efforts arise from the fact that long‐term studies and in vivo observation of already available materials indicate that the requirements for effective implants are not being fully met.…”

Section: Introductionmentioning

confidence: 99%

Sonochemical coating as an effective method of polymeric nonwovens functionalization

Kołbuk

Urbanek

Denis

et al. 2019

J Biomedical Materials Res

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A surface of polymeric nonwovens may be coated with various types of nanoparticles for medical applications, filtration, and so forth. However, quite often methods used for surface modification are difficult to scale up or are not applicable for polymers. In this article, we present one-step process enabling nonwovens functionalization. Poly (L-lactide-co-glicolide) (PLGA) nonwovens were prepared by electrospinning process and coated with hydroxyapatite nanoparticles (HAp) using ultrasonic processing. The effect of the process was evaluated with various techniques. HAp layer was successfully attached without loss of structural properties of HAp or PLGA nonwovens. The analysis confirmed the decrease of hydrophobicity of coated nonwoven, as well as its biocompatibility, making this material valuable from the perspective of medical applications. The sonochemical functionalization of polymeric nonwovens may be considered as an effective and economic method, enhancing surface properties of electrospun nonwovens for various applications.

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Section: Introductionmentioning

confidence: 99%

Sonochemical coating as an effective method of polymeric nonwovens functionalization

Kołbuk

Urbanek

Denis

et al. 2019

J Biomedical Materials Res

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“…Therefore, together with others, we propose that 3D culturing is a better option to assess the invasive ability of cancer cells [46,47].…”

Section: Discussionmentioning

confidence: 92%

Thermal stress involved in TRPV2 promotes tumorigenesis through the pathway of PI3K/Akt/mTOR in esophageal squamous cell carcinoma

Huang¹,

Li²,

Tian³

et al. 2020

Preprint

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BackgroundEsophageal squamous cell carcinoma (ESCC) is a leading cause of cancer death worldwide. Although the exposure of esophageal mucosa to heat stimuli has long been recognized as an important risk for the initiation and development of ESCC, its underlying mechanisms remain uncharacterized. MethodsWestern blotting and immunofluorescence were used to detect the expression and localization of transient receptor potential vanilloid receptor 2 (TRPV2) in the ESCC cells. The cancerous behaviors of the ESCC cells were evaluated by a single-cell culturing assay, a wound healing assay, a 3D culturing assay and a tube formation assay respectively. In vivo tumorigenicity and metastasis of the ESCC cells were examined via xenograft nude mouse models. Western blotting and IHC were performed to determine the expression profiles of TRPV2 in the ESCC patient tissues. TRPV2 knocked-out ESCC cell line was established using CRISPR-Cas9 gene editing technique.ResultsHere, we found that the expression of TRPV2, one of the thermally sensitive TRP family members, was upregulated in both ESCC cells and clinical samples. We further showed that activation of TRPV2 by recurrent acute thermal stress (54°C, a temperature unexpectedly much lower than those in many dietary modalities) or O1821 (20 μM), a TRPV2 agonist, promoted cancerous behaviors in ESCC cells. The proangiogenic capacity of the heat-challenged ESCC cells was also found to be enhanced profoundly in the tube formation assay; both tumor formation and metastasis that originated from the cells were substantially promoted in nude mouse models upon the activation of TRPV2. These effects were inhibited significantly by tranilast (120 μM), a TRPV2 inhibitor, and abolished by TRPV2 knock-out using CRISPR-Cas9 gene editing. Conversely, overexpression of TRPV2 by transfection of TRPV2 DNA into NE2 cells, could switch the cells to tumorigenesis upon activation of TRPV2. Mechanistically, the driving role of TRPV2 in the progression of ESCC is mainly regulated by the PI3K/Akt/mTOR signaling pathway. Application of a pan-PI3K/mTOR inhibitor and/or a PTEN activator resulted in markedly reduced ESCC cell proliferation. ConclusionsOur study first proved that TRPV2 plays an important role in the tumorigenesis of ESCC upon thermal stress. We revealed that TRPV2-PI3K/Akt/mTOR is a novel and promising target for the prevention and treatment of ESCC.

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“…It also does not adequately consider the influence of the three-dimensional (3D) microenvironment under natural physiological conditions on its internal response and affects dose effectiveness and dose toxicity prediction data 17 . The inability to provide living tissue structure, mechanical/biochemical signals, and cell behavior has informative limitations 18 , 19 .…”

Section: Introductionmentioning

confidence: 99%

Applications of Engineered Skin Tissue for Cosmetic Component and Toxicology Detection

Wang,

Zhang,

Hao

et al. 2024

Cell Transplant

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The scale of the cosmetic market is increasing every day. There are many safety risks to cosmetics, but they benefit people at the same time. The skin can become red, swollen, itchy, chronically toxic, and senescent due to the misuse of cosmetics, triggering skin injuries, with contact dermatitis being the most common. Therefore, there is an urgent need for a system that can scientifically and rationally detect the composition and perform a toxicological assessment of cosmetic products. Traditional detection methods rely on instrumentation and method selection, which are less sensitive and more complex to perform. Engineered skin tissue has emerged with the advent of tissue engineering technology as an emerging bioengineering technology. The ideal engineered skin tissue is the basis for building good in vitro structures and physiological functions in this field. This review introduces the existing cosmetic testing and toxicological evaluation methods, the current development status, and the types and characteristics of engineered skin tissue. The application of engineered skin tissue in the field of cosmetic composition detection and toxicological evaluation, as well as the different types of tissue engineering scaffold materials and three-dimensional (3D) organoid preparation approaches, is highlighted in this review to provide methods and ideas for constructing the next engineered skin tissue for cosmetic raw material component analysis and toxicological evaluation.

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Three‐dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion

Cited by 9 publications

References 30 publications

Sonochemical coating as an effective method of polymeric nonwovens functionalization

Sonochemical coating as an effective method of polymeric nonwovens functionalization

Thermal stress involved in TRPV2 promotes tumorigenesis through the pathway of PI3K/Akt/mTOR in esophageal squamous cell carcinoma

Applications of Engineered Skin Tissue for Cosmetic Component and Toxicology Detection

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