Three-dimensional culture using human plasma-medium gel with fragmin/protamine microparticles for proliferation of various human cells

Kishimoto, Satoko; Takikawa, Makoto; Takikawa, Megumi; Sumi, Yuki; Nakamura, Shingo; Fujita, Masanori; Sato, Toshinori; Kiyosawa, Tomoharu

doi:10.1007/s10616-013-9628-5

Cited by 6 publications

(10 citation statements)

References 18 publications

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“…Low concentration of ox-LDL (100 nmol/L) could induce the upregulation of MMP-2 and -9, and miR-29b, inducing the migration of SMCs. Interleukin-3 (IL-3) has been shown to promote cell proliferation and migration in vascular disease (Kishimoto et al 2014). Mir-9 might control the IL-3-induced proliferation and migration of SMCs (Lee et al 2015).…”

Section: Discussionmentioning

confidence: 99%

MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2

Shen

Song²,

et al. 2017

Cytotechnology

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The phenotypic transformation and dysfunctions of vascular smooth muscle cells (SMCs) such as abnormality proliferation and apoptosis are key pathological basis of atherosclerosis. The recent study aimed to detect the role of miR-29b in phenotypic transformation of SMCs. In this study, we investigated the expression level of miR-29b and MMP-2 in acute coronary syndrome (ACS) patients, verified whether MMP-2 is the target gene of miR-29b by luciferase reporter gene system, and explored the role of miR-29b in the viability and apoptosis of SMCs. We found that the plasma level of miR-29b was significantly downregulated to 56% of controls (p < 0.01). The plasma level of MMP-2 in health controls was 34.9 ± 6.9 ng/mL, and that it significantly increased to 46.2 ± 13.2 ng/mL in ACS patients. MMP-2 is a target gene of miR-29b. The overexpression of miR-29b significantly downregulated the expression of MMP-2 mRNA and protein. miR-29b mimics inhibited the cell viability of SMCs, and cell apoptosis was significantly enhanced compared with the NC group, especially in the early stage. In the presence of MMP-2 inhibitor SB-3CT, the cell viability and apoptosis of SMC cells were significantly reduced and enhanced, respectively, while the miR-29b -inhibited cell viability and -induced cell apoptosis were not significantly changed. Taken together, miR-29b was downregulated in ACS patients. MiR-29 mimics inhibits cell viability and promotes cell apoptosis via directly targeting on MMP-2, which could be a potentially promising therapy target for cardiovascular diseases.

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Section: Discussionmentioning

confidence: 99%

MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2

Shen

Song²,

et al. 2017

Cytotechnology

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“…[ 44 ] 3D cell culture gels induce higher cell proliferation than their 2D culture counterparts for several types of cells, including smooth muscle, endothelial, and fi broblast cells. [ 45 ] This study, however, does not address submicrometer topography, but rather develops a model for 3D cell culture with a fl exible scaffold that has potential to protect the culture during transplantation and during other mechanical stress exposures that require signifi cant material elasticity. An inherent limitation of sub-micrometer laser 3D printing is that the printing time increases as the resolution increases into the sub-micrometer range.…”

Section: Discussionmentioning

confidence: 99%

Laser 3D Printing with Sub‐Microscale Resolution of Porous Elastomeric Scaffolds for Supporting Human Bone Stem Cells

Petrochenko

Torgersen

Gruber

et al. 2014

Adv Healthcare Materials

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A reproducible method is needed to fabricate 3D scaffold constructs that results in periodic and uniform structures with precise control at sub-micrometer and micrometer length scales. In this study, fabrication of scaffolds by two-photon polymerization (2PP) of a biodegradable urethane and acrylate-based photoelastomer is demonstrated. This material supports 2PP processing with sub-micrometer spatial resolution. The high photoreactivity of the biophotoelastomer permits 2PP processing at a scanning speed of 1000 mm s(-1), facilitating rapid fabrication of relatively large structures (>5 mm(3)). These structures are custom printed for in vitro assay screening in 96-well plates and are sufficiently flexible to enable facile handling and transplantation. These results indicate that stable scaffolds with porosities of greater than 60% can be produced using 2PP. Human bone marrow stromal cells grown on 3D scaffolds exhibit increased growth and proliferation compared to smooth 2D scaffold controls. 3D scaffolds adsorb larger amounts of protein than smooth 2D scaffolds due to their larger surface area; the scaffolds also allow cells to attach in multiple planes and to completely infiltrate the porous scaffolds. The flexible photoelastomer material is biocompatible in vitro and is associated with facile handling, making it a viable candidate for further study of complex 3D-printed scaffolds.

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“…There are several advantages of developing a platform with plasma as the framework. Being enriched with growth factors and proteins that contribute to the regenerative process, a plasma derived microenvironment potentiates cell proliferation, migration, and differentiation mimicking in vivo conditions, hence enhancing the clinical utility of the model (80)(81)(82)(83). Of note, the use of human plasma as a matrix not only supports cell structure, but also serves as a reservoir for nutrients, growth factors, cytokines, extracellular vesicles, and signaling molecules (84)(85)(86) that help with the development of more relevant human cancer models.…”

Section: Immune Cell Infiltration Into 3d Matrices Has Been Observed mentioning

confidence: 99%

Bioengineering a novel 3D in-vitro model to recreate physiological oxygen levels and tumor-immune interactions

Bhattacharya

Calar

Evans

et al. 2019

Preprint

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Oxygen deprivation within tumors is one of the most prevalent causes of resilient cancer cell survival and increased immune evasion in breast cancer (BCa). Current in vitro models do not adequately mimic physiological oxygen levels relevant to breast tissue and its tumor-immune interactions. Here, we propose an approach to engineer a three-dimensional (3D) model (named 3D engineered oxygen, 3D-O) that supports growth of BCa cells and generates physio-and pathophysiological oxygen levels. Low oxygen-induced changes within the 3D-O model supported known tumor hypoxia characteristics such as reduced BCa cell proliferation, increased extracellular matrix protein expression, increased extracellular vesicle secretion and enhanced immune surface marker expression on BCa cells. We further demonstrated that low oxygeninduced changes mimicked tumor-immune interactions leading to immune evasion mechanisms. CD8+ T cell infiltration was significantly impaired under pathophysiological oxygen levels andwe were able to establish that hypoxia inhibition re-sensitize BCa cells to cytotoxic CD8+ T cells.Therefore, our novel 3D-O model could serve as a promising platform for the evaluation of immunological events and as a drug-screening platform tool to overcome hypoxia-driven immune evasion.

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Three-dimensional culture using human plasma-medium gel with fragmin/protamine microparticles for proliferation of various human cells

Cited by 6 publications

References 18 publications

MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2

MiR-29b mimics promotes cell apoptosis of smooth muscle cells via targeting on MMP-2

Laser 3D Printing with Sub‐Microscale Resolution of Porous Elastomeric Scaffolds for Supporting Human Bone Stem Cells

Bioengineering a novel 3D in-vitro model to recreate physiological oxygen levels and tumor-immune interactions

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