Three-Dimensional Culture Promotes the Differentiation of Human Dental Pulp Mesenchymal Stem Cells Into Insulin-Producing Cells for Improving the Diabetes Therapy

Xu, Bingbing; Fan, Daoyang; Yun-feng, Zhao; Li, Jing; Wang, Zhendong; Wang, Jianhua; Wang, Xiuwei; Guan, Zhiyong; Niu, Bo

doi:10.3389/fphar.2019.01576

Cited by 39 publications

(33 citation statements)

References 32 publications

(37 reference statements)

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“…Furthermore, the advantages of hDPSC have been reported by several investigators i.e. the multilineage differentiation potential, the capacity for autologous and allogenic transplantation [16], the abundance in source and less ethical issue both in research and clinical translation [17].…”

Section: Introductionmentioning

confidence: 99%

Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells

et al. 2020

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Background: Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp-derived stem cell (hDPSC)-derived IPCs by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. Results: The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG and ALGPA-encapsulated hDPSC-derived IPCs maintained viability for at least 336 h and sustained pancreatic endoderm marker (NGN3), pancreatic islet markers (NKX6.1, MAF-A, ISL-1, GLUT-2 and INSULIN), and intracellular pro-insulin and insulin expressions for at least 14 days. Functional analysis revealed a glucoseresponsive C-peptide secretion of ALG-and ALGPA-encapsulated hDPSC-derived IPCs at 14 days post-encapsulation. Conclusion: ALG and ALGPA encapsulations efficiently preserved the viability and functionality of hDPSC-derived IPCs in vitro and could be the potential transplantation platform for further clinical application.

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Section: Introductionmentioning

confidence: 99%

Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells

et al. 2020

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“…Low-temperature Matrigel suspension and injection was effective for establishing an orthotopic mouse model of pancreatic cancer (Jiang et al, 2014 ). The three-dimensional culture of hDPSCs in Matrigel promotes the differentiation of DPSCs into insulin-producing Cells (Xu et al, 2020a ). Matrigel could also be used for the renal subcapsular transplant of native mouse islets and pancreatic progenitor-like cells (Li et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases

Yuan

Lin

et al. 2020

Front. Bioeng. Biotechnol.

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“…The embryonic stem cells cultured in 3D as embryoid bodies exhibit increased abilities to differentiate to chondrocytes compared to being cultured in the monolayer (Tanaka et al, 2004). The natural cell-cell intercommunication and cell-extracellular matrix interaction in the 3D structure, mimicking the in vivo microenvironment, can influence the hDPSC properties and their ability to differentiate toward different cell lineages (Riccio et al, 2010;Xu et al, 2020). Therefore, depending on in vivo or in vitro microenvironment, cellular intercommunication could be affected and GMSCs could differently respond to the presence of the scaffold.…”

Section: Discussionmentioning

confidence: 99%

Growth and Osteogenic Differentiation of Discarded Gingiva-Derived Mesenchymal Stem Cells on a Commercial Scaffold

Cristaldi

Mauceri

Campisi

et al. 2020

Front. Cell Dev. Biol.

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Background: In periodontal patients with jawbone resorption, the autologous bone graft is considered a "gold standard" procedure for the placing of dental prosthesis; however, this procedure is a costly intervention and poses the risk of clinical complications. Thanks to the use of adult mesenchymal stem cells, smart biomaterials, and active biomolecules, regenerative medicine and bone tissue engineering represent a valid alternative to the traditional procedures. Aims: In the past, mesenchymal stem cells isolated from periodontally compromised gingiva were considered a biological waste and discarded during surgical procedures. This study aims to test the osteoconductive activity of FISIOGRAFT Bone Granular R and Matriderm R collagen scaffolds on mesenchymal stem cells isolated from periodontally compromised gingiva as a low-cost and painless strategy of autologous bone tissue regeneration. Materials and Methods: We isolated human mesenchymal stem cells from 22 healthy and 26 periodontally compromised gingival biopsy tissues and confirmed the stem cell phenotype by doubling time assay, colony-forming unit assay, and expression of surface and nuclear mesenchymal stem cell markers, respectively by cytofluorimetry and realtime quantitative PCR. Healthy and periodontally compromised gingival mesenchymal stem cells were seeded on FISIOGRAFT Bone Granular R and Matriderm R scaffolds, and in vitro cell viability and bone differentiation were then evaluated. Results: Even though preliminary, the results demonstrate that FISIOGRAFT Bone Granular R is not suitable for in vitro growth and osteogenic differentiation of healthy and periodontally compromised mesenchymal stem cells, which, instead, are able to grow, homogeneously distribute, and bone differentiate in the Matriderm R collagen scaffold.

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Three-Dimensional Culture Promotes the Differentiation of Human Dental Pulp Mesenchymal Stem Cells Into Insulin-Producing Cells for Improving the Diabetes Therapy

Cited by 39 publications

References 32 publications

Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells

Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells

The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases

Growth and Osteogenic Differentiation of Discarded Gingiva-Derived Mesenchymal Stem Cells on a Commercial Scaffold

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