Three-dimensional culture of small-diameter vascular grafts

Elliott, Morgan B.; Gerecht, Sharon

doi:10.1039/c6tb00024j

Cited by 38 publications

(33 citation statements)

References 48 publications

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“…6A). This indicates the formation of a muscular tunica media with circumferential orientation, which is typically seen in native aortas (23).…”

Section: Development Of Natural Polymer Conduits With Controlled Surfacementioning

confidence: 75%

“…Attempts to fabricate natural polymer-derived sdVGs have historically been plagued by limited mechanical properties (22,23). A widespread method to improve mechanical properties of grafts is the incorporation of vascular smooth muscle cells (vSMCs) and fibroblasts that deposit extracellular matrix (ECM) molecules, including collagen and elastin, before implantation (22,24,25), a decellularized example of which is proceeding through clinical trials (26).…”

Section: Significancementioning

confidence: 99%

“…In Vitro and In Vivo Endothelialization. Critical to sdVG applications, longitudinally aligned ECs reduce platelet activation and adhesion, aid in fibrinolysis, and prevent intimal hyperplasia (23,(57)(58)(59). Thus, we sought to test whether the natural sdVGs guide rapid endothelization.…”

Section: Development Of Natural Polymer Conduits With Controlled Surfacementioning

confidence: 99%

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Regenerative and durable small-diameter graft as an arterial conduit

Elliott

Ginn

Fukunishi

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Despite significant research efforts, clinical practice for arterial bypass surgery has been stagnant, and engineered grafts continue to face postimplantation challenges. Here, we describe the development and application of a durable small-diameter vascular graft with tailored regenerative capacity. We fabricated small-diameter vascular grafts by electrospinning fibrin tubes and poly(e-caprolactone) fibrous sheaths, which improved suture retention strength and enabled long-term survival. Using surface topography in a hollow fibrin microfiber tube, we enable immediate, controlled perfusion and formation of a confluent endothelium within 3-4 days in vitro with human endothelial colony-forming cells, but a stable endothelium is noticeable at 4 weeks in vivo. Implantation of acellular or endothelialized fibrin grafts with an external ultrathin poly(e-caprolactone) sheath as an interposition graft in the abdominal aorta of a severe combined immunodeficient Beige mouse model supports normal blood flow and vessel patency for 24 weeks. Mechanical properties of the implanted grafts closely approximate the native abdominal aorta properties after just 1 week in vivo. Fibrin mediated cellular remodeling, stable tunica intima and media formation, and abundant matrix deposition with organized collagen layers and wavy elastin lamellae. Endothelialized grafts evidenced controlled healthy remodeling with delayed and reduced macrophage infiltration alongside neo vasa vasorum-like structure formation, reduced calcification, and accelerated tunica media formation. Our studies establish a small-diameter graft that is fabricated in less than 1 week, mediates neotissue formation and incorporation into the native tissue, and matches the native vessel size and mechanical properties, overcoming main challenges in arterial bypass surgery.small-diameter vascular graft | infrarenal abdominal aorta mouse model | electrospinning | hydrogel | endothelial colony-forming cells

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“…6A). This indicates the formation of a muscular tunica media with circumferential orientation, which is typically seen in native aortas (23).…”

Section: Development Of Natural Polymer Conduits With Controlled Surfacementioning

confidence: 75%

Section: Significancementioning

confidence: 99%

See 1 more Smart Citation

Regenerative and durable small-diameter graft as an arterial conduit

Elliott

Ginn

Fukunishi

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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“…Intima, the innermost layer is a thromboresistent confluent monolayer of ECs and is attached to a basement membrane (40∼120 nm). Media, the middle layer, is comprised of a dense population of concentrically organized SMCs with bands or fibers of elastic tissues, and adventitia, the outermost layer, is a collagenous ECM containing mainly fibroblasts and perivascular nerves . In contrast, the capillaries only consist of EC layer.…”

Section: D Bioprinting Of Organsmentioning

confidence: 99%

3D Bioprinting for Organ Regeneration

Cui

Nowicki

Fisher

et al. 2016

Adv Healthcare Materials

427

392

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Regenerative medicine holds the promise of engineering functional tissues or organs to heal or replace abnormal and necrotic tissues/organs, offering hope for filling the gap between organ shortage and transplantation needs. Three-dimensional (3D) bioprinting is evolving into an unparalleled bio-manufacturing technology due to its high-integration potential for patient-specific designs, precise and rapid manufacturing capabilities with high resolution, and unprecedented versatility. It enables precise control over multiple compositions, spatial distributions, and architectural accuracy/complexity, therefore achieving effective recapitulation of microstructure, architecture, mechanical properties, and biological functions of target tissues and organs. Here we provide an overview of recent advances in 3D bioprinting technology, as well as design concepts of bioinks suitable for the bioprinting process. We focus on the applications of this technology for engineering living organs, focusing more specifically on vasculature, neural networks, the heart and liver. We conclude with current challenges and the technical perspective for further development of 3D organ bioprinting.

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“…Doğal kaynaklı biyomalzemelerden oluşan iskele yapıları ise ilk olarak kendiliğinden biyouyumluluk özelliği göste-rerek avantaj sağlarlar; ancak bu yapılardan özellikle deselülerize yapılarda görülen zayıf mekanik özellik-ler dezavantaj yaratmaktadır. 4 Doğal kaynaklı biyomalzeme örneklerinden deselülerize matriks yapıları yakın zamanda yapı-lan doku mühendisliği çalışmalarında karşımıza çıkmaktadır. Temel olarak deselülerize yapılar, insan ya da insan dışında başka kaynaklardan elde edilen dokuların hücre bileşenlerinden arındırıl-mış şeklini ifade etmektedir.…”

Section: Doku Mühendisliği Ve Uygulama Alanlarıunclassified