Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers

Ishaug-Riley, Susan L.; Crane, Genevieve M.; Yaszemski, Michael J.; Mikos, Antonios G.

doi:10.1016/s0142-9612(98)00021-0

Cited by 456 publications

(283 citation statements)

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“…Additionally, cells grown on TCPS exhibited similar temporal trends in ALP activity and mineralization as in a previous report [6], suggesting that PEEK-SP accelerated osteoblast differentiation rather than smooth PEEK and Ti6Al4V causing delayed differentiation. One potential explanation for the initially increased cell proliferation on PEEK-SP is that the increased surface area effectively decreased the seeding density of cells, which could have facilitated greater cell proliferation at early time points [20,54]. However, this increase in surface area and early proliferation did not translate to greater cell numbers at later time points (Fig.…”

Section: Discussionmentioning

confidence: 99%

Do Surface Porosity and Pore Size Influence Mechanical Properties and Cellular Response to PEEK?

Torstrick

Evans

Stevens

et al. 2016

Clinical Orthopaedics &Amp; Related Research

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Background Despite its widespread use in orthopaedic implants such as soft tissue fasteners and spinal intervertebral implants, polyetheretherketone (PEEK) often suffers from poor osseointegration. Introducing porosity can overcome this limitation by encouraging bone ingrowth; however, the corresponding decrease in implant strength can potentially reduce the implant's ability to bear physiologic loads. We have previously shown, using a single pore size, that limiting porosity to the surface of PEEK implants preserves strength while supporting in vivo osseointegration. However, additional work is needed to investigate the effect of pore size on both the mechanical properties and cellular response to PEEK. Questions/purposes (1) Can surface porous PEEK (PEEK-SP) microstructure be reliably controlled? (2) What is the effect of pore size on the mechanical properties of PEEK-SP? (3) Do surface porosity and pore size influence the cellular response to PEEK? Methods PEEK-SP was created by extruding PEEK through NaCl crystals of three controlled ranges: 200 to 312, 312 to 425, and 425 to 508 lm. Micro-CT was used to characterize the microstructure of PEEK-SP. Tensile, fatigue, and interfacial shear tests were performed to compare the mechanical properties of PEEK-SP with injectionmolded PEEK (PEEK-IM). The cellular response to PEEK-SP, assessed by proliferation, alkaline phosphatase activity, vascular endothelial growth factor production, and calcium content of osteoblast, mesenchymal stem cell, and preosteoblast (MC3T3-E1) cultures, was compared with that of machined smooth PEEK and Ti6Al4V. Results Micro-CT analysis showed that PEEK-SP layers possessed pores that were 284 ± 35 lm, 341 ± 49 lm, and 416 ± 54 lm for each pore size group. Porosity and

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Section: Discussionmentioning

confidence: 99%

Do Surface Porosity and Pore Size Influence Mechanical Properties and Cellular Response to PEEK?

Torstrick

Evans

Stevens

et al. 2016

Clinical Orthopaedics &Amp; Related Research

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“…The static method resulted in a statistically lower uniformity, consistent with the nonuniform spatial cell distributions reported by several groups. 4,6,21,22 This low uniformity may be explained by the difficulty in evenly distributing the small volume of cell suspension over the scaffold surface and by the intrinsically weak mechanism of infusion ͑i.e., via gravity and capillary action͒.…”

Section: Discussionmentioning

confidence: 99%

Effect of surface acoustic waves on the viability, proliferation and differentiation of primary osteoblast-like cells

Li¹,

Friend²,

Yeo³

et al. 2009

Biomicrofluidics

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Surface acoustic waves ͑SAWs͒ have been used as a rapid and efficient technique for driving microparticles into a three-dimensional scaffold matrix, raising the possibility that SAW may be effective in seeding live cells into scaffolds, that is, if the cells were able to survive the infusion process. Primary osteoblast-like cells were used to specifically address this issue: To investigate the effects of SAW on the cells' viability, proliferation, and differentiation. Fluorescence-labeled osteoblastlike cells were seeded into polycaprolactone scaffolds using the SAW method with a static method as a control. The cell distribution in the scaffold was assessed through image analysis. The cells were far more uniformly driven into the scaffold with the SAW method compared to the control, and the seeding process with SAW was also significantly faster: Cells were delivered into the scaffold in seconds compared to the hour-long process of static seeding. Over 80% of the osteoblastlike cells were found to be viable after being treated with SAW at 20 MHz for 10-30 s with an applied power of 380 mW over a wide range of cell suspension volumes ͑10-100 ᐉ͒ and cell densities ͑1000-8000 cells/ ᐉ͒. After determining the optimal cell seeding parameters, we further found that the treated cells offered the same functionality as untreated cells. Taken together, these results show that the SAW method has significant potential as a practical scaffold cell seeding method for tissue and orthopedic engineering.

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“…These were based on the utilization of supports, as gels and sponges of collagen type I, PLGA foam, DegraPol, agarose, Matrigel, or methylcellulose. 7,10,15,16,18,20,22,25,26,30,32,34,37,39 Normal human osteogenic cells, osteoblasts from prepubertal-age donors, or bone marrow stromal fibroblasts adhering to scaffolding of HAP/ TC, 16,20,25,26,30,34 collagraft, 26 collagen sponges, 15,39 or bone chips 5 were utilized to produce specifically 3D structures designed for direct implantation in vivo.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional cultures of normal human osteoblasts: proliferation and differentiation potential in vitro and upon ectopic implantation in nude mice

Ferrera

Poggi

Biassoni

et al. 2002

Bone

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Three-dimensional culture of rat calvarial osteoblasts in porous biodegradable polymers

Cited by 456 publications

References 0 publications

Do Surface Porosity and Pore Size Influence Mechanical Properties and Cellular Response to PEEK?

Do Surface Porosity and Pore Size Influence Mechanical Properties and Cellular Response to PEEK?

Effect of surface acoustic waves on the viability, proliferation and differentiation of primary osteoblast-like cells

Three-dimensional cultures of normal human osteoblasts: proliferation and differentiation potential in vitro and upon ectopic implantation in nude mice

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