Three-dimensional conditions in a perfusion bioreactor to support maturation of human amnion epithelial stem cells into functional hepatocyte-like cells

Campinoti, Sara; Goudarzi, Negin; Almeida, Bruna Rocha de; Cox, I.Jan; Gramignoli, Roberto; Urbani, Luca

doi:10.1016/s0168-8278(22)01791-3

Cited by 1 publication

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“…Decellularized tissue-derived ECM (dECM) hydrogel has emerged as a promising alternative to the conventional matrices in organoid culture. 141 – 146 dECM hydrogel is highly enriched with various ECM proteins, including collagen, fibronectin, laminin, and proteoglycan, thusproviding ECM networks for cell growth and differentiation. 136 , 147 – 149 The presence of tissue-specific ECM components in dECM hydrogel has also been underscored as organoids may require different ECM cues in a tissue dependent manner.…”

Section: Advanced Organoid Culture Platformsmentioning

confidence: 99%

Advanced lung organoids for respiratory system and pulmonary disease modeling

Joo,

Min,

Cho

2024

J Tissue Eng

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Amidst the recent coronavirus disease 2019 (COVID-19) pandemic, respiratory system research has made remarkable progress, particularly focusing on infectious diseases. Lung organoid, a miniaturized structure recapitulating lung tissue, has gained global attention because of its advantages over other conventional models such as two-dimensional (2D) cell models and animal models. Nevertheless, lung organoids still face limitations concerning heterogeneity, complexity, and maturity compared to the native lung tissue. To address these limitations, researchers have employed co-culture methods with various cell types including endothelial cells, mesenchymal cells, and immune cells, and incorporated bioengineering platforms such as air-liquid interfaces, microfluidic chips, and functional hydrogels. These advancements have facilitated applications of lung organoids to studies of pulmonary diseases, providing insights into disease mechanisms and potential treatments. This review introduces recent progress in the production methods of lung organoids, strategies for improving maturity, functionality, and complexity of organoids, and their application in disease modeling, including respiratory infection and pulmonary fibrosis.

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