Three-Dimensional Collagen Matrix Scaffold Implantation as a Liver Regeneration Strategy

León-Mancilla, Benjamín; Martínez-Castillo, Moisés; Medina-Ávila, Zaira; Pérez‐Torres, Armando; García-Loya, Jorge; Alfaro-Cruz, Ana; Piña-Barba, Cristina; Gutiérrez-Reyes, Gabriela

doi:10.3791/62697

Cited by 2 publications

(4 citation statements)

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“…Furthermore, the recovery of biochemical levels is reported to begin 3 days after the surgical process ( 15 , 50 , 51 ). In our study, different metabolites directed toward liver function were evaluated, and in general, the biochemical values were within the reference ranges reported for Wistar rats ( 14 , 52 ). Our study revealed a reduction of ALB in the PH + CMS group.…”

Section: Discussionsupporting

confidence: 65%

“…To obtain the CMS, we collected the bovine femoral condyle, as previously reported ( 14 ). Briefly, the selected samples of 3 cm × 3 cm were carefully dissected and washed with water using anionic detergent.…”

Section: Methodsmentioning

confidence: 99%

“…Partial hepatectomy (40% of the left lobe) and CMS implantation were performed, as previously described ( 14 ). Briefly, the animals were sedated using intramuscular doses of ketamine (35 mg/kg) and xylazine (2.5 mg/kg) ( PiSA , Agropecuaria , Mex).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, we recently reported the implantation of CMS in the livers of rats. However, the full histological events and characterization of the model were not reported ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

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Xenoimplant of Collagen Matrix Scaffold in Liver Tissue as a Niche for Liver Cells

et al. 2022

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Hepatitis C virus-induced liver damage, chronic liver damage due to alcohol, and non-alcoholic liver disease-induced cellular alterations promote fibrosis, cirrhosis, and/or hepatocellular carcinoma. The recommended therapeutic option for advanced liver damage is liver transplantation. Extracellular matrix scaffolds have been evaluated as an alternative for tissue restoration. Studies on the biocompatibility and rejection of synthetic and natural scaffolds as an alternative to organ transplantation have been evaluated. Our group has recently described the xenoimplant of collagen matrix scaffold (CMS) in a rat model. However, no complete macroscopic and histological description of the liver parenchyma at the initial (day 3), intermediate (day 14), and advanced (day 21) stages has been obtained. In this study, we described and compared liver tissue from the CMS zone (CZ, CMS, and liver parenchyma), liver tissue from the normal zone (liver parenchyma close to the CMS), and basal tissue (resected tissue from the CMS implantation site). Our data strongly suggest that the collagen matrix xenoimplant is a good niche for hepatocytes, with no rejection, and does not affect liver function tests. The liver can regenerate after damage, but this capacity is inhibited in a chronic injury. At present, the use of CMS after liver damage has not been reported. This biomaterial could be a novel alternative in the field of regenerative medicine for liver diseases.

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Section: Discussionsupporting

confidence: 65%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…In addition, we recently reported the implantation of CMS in the livers of rats. However, the full histological events and characterization of the model were not reported ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations