Three-dimensional changes of noses after transplantation of implant-type tissue-engineered cartilage for secondary correction of cleft lip–nose patients

Hoshi, Kazuto; Fujihara, Yuko; Saijo, Hideto; Kurabayashi, Kumiko; Suenaga, Hideyuki; Asawa, Yukiyo; Nishizawa, Satoru; Kanazawa, Sanshiro; Uto, Sakura; Inaki, Ryoko; Matsuyama, Mariko; Sakamoto, Tomoaki; Watanabe, Makoto; Sugiyama, Madoka; Yonenaga, Kazumichi; Hikita, Atsuhiko; Takato, Tsuyoshi

doi:10.1016/j.reth.2017.09.001

Cited by 25 publications

(25 citation statements)

References 19 publications

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“…Hoshi et al developed an implant-type tissue-engineered cartilage using a PLA based scaffold and evaluated it clinically by inserting it into subcutaneous areas of nasal dorsum in three patients to correct cleft lip–nose deformity. Subsequently, one year after implantation, the maintenance of the morphology in the dorsum and apex of the nose of the patients was confirmed [35]. Similar results were also reported by Puwanun et al but using biodegradable electrospun PCL scaffolds with the ability to support bone-forming cells and within cleft palate bone defects [36].…”

Section: Biomaterials For Soft and Hard Cleft Tissue Repairsupporting

confidence: 66%

Biomaterials for Cleft Lip and Palate Regeneration

Martín-del-Campo

Rosales-Ibáñez

Rojo

2019

IJMS

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Craniofacial bone defect anomalies affect both soft and hard tissues and can be caused by trauma, bone recessions from tumors and cysts, or even from congenital disorders. On this note, cleft/lip palate is the most prevalent congenital craniofacial defect caused by disturbed embryonic development of soft and hard tissues around the oral cavity and face area, resulting in most cases, of severe limitations with chewing, swallowing, and talking as well as problems of insufficient space for teeth, proper breathing, and self-esteem problems as a consequence of facial appearance. Spectacular advances in regenerative medicine have arrived, giving new hope to patients that can benefit from new tissue engineering therapies based on the supportive action of 3D biomaterials together with the synergic action of osteo-inductive molecules and recruited stem cells that can be driven to the process of bone regeneration. However, few studies have focused on the application of tissue engineering to the regeneration of the cleft/lip and only a few have reported significant advances to offer real clinical solutions. This review provides an updated and deep analysis of the studies that have reported on the use of advanced biomaterials and cell therapies for the regeneration of cleft lip and palate regeneration.

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Section: Biomaterials For Soft and Hard Cleft Tissue Repairsupporting

confidence: 66%

Biomaterials for Cleft Lip and Palate Regeneration

Martín-del-Campo

Rosales-Ibáñez

Rojo

2019

IJMS

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“…Then, we produced implant-type tissue-engineered cartilage and performed clinical trials in three patients. 36 The treatment showed efficacy at 1 year after transplantation in all patients, suggesting that GFAP in the auricular chondrocyte culture supernatant is useful as a quality index. This criterion is adopted for quality control in an ongoing clinical study aiming at industrialization.…”

Section: Discussionmentioning

confidence: 84%

Glial Fibrillary Acidic Protein as Biomarker Indicates Purity and Property of Auricular Chondrocytes

Nishizawa

Kanazawa

Fujihara

et al. 2020

BioResearch Open Access

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Instead of the silicone implants previously used for repair and reconstruction of the auricle and nose lost due to accidents and disease, a new treatment method using tissue-engineered cartilage has been attracting attention. The quality of cultured cells is important in this method because it affects treatment outcomes. However, a marker of chondrocytes, particularly auricular chondrocytes, has not yet been established. The objective of this study was to establish an optimal marker to evaluate the quality of cultured auricular chondrocytes as a cell source of regenerative cartilage tissue. Gene expression levels were comprehensively compared using the microarray method between human undifferentiated and dedifferentiated auricular chondrocytes to investigate a candidate quality control index with an expression level that is high in differentiated cells, but markedly decreases in dedifferentiated cells. We identified glial fibrillary acidic protein (GFAP) as a marker that decreased with serial passages in auricular chondrocytes. GFAP was not detected in articular chondrocytes, costal chondrocytes, or fibroblasts, which need to be distinguished from auricular chondrocytes in cell cultures. GFAP mRNA expression was observed in cultured auricular chondrocytes, and GFAP protein levels were also measured in the cell lysates and culture supernatants of these cells. However, GFAP levels detected from mRNA and protein in cell lysates were significantly decreased by increases in the incubation period. In contrast, the amount of protein in the cell supernatant was not affected by the incubation period. Furthermore, the protein level of GFAP in the supernatants of cultured cells correlated with the in vitro and in vivo production of the cartilage matrix by these cells. The productivity of the cartilage matrix in cultured auricular chondrocytes may be predicted by measuring GFAP protein levels in the culture supernatants of these cells. Thus, GFAP is regarded as a marker of the purity and properties of cultured auricular chondrocytes.

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“…Hoshi et al utilized a collagen scaffold‐based tissue‐engineered cartilage to augment the nasal dorsum in patients with cleft lip‐nose deformity. Patients experienced improved nasal shape and a clinical trial was initiated for further investigation 21 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

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While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

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Three-dimensional changes of noses after transplantation of implant-type tissue-engineered cartilage for secondary correction of cleft lip–nose patients

Cited by 25 publications

References 19 publications

Biomaterials for Cleft Lip and Palate Regeneration

Biomaterials for Cleft Lip and Palate Regeneration

Glial Fibrillary Acidic Protein as Biomarker Indicates Purity and Property of Auricular Chondrocytes

Tissue engineering applications in otolaryngology—The state of translation

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